Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6528 and GFF6529 are separated by 13 nucleotides GFF6529 and GFF6530 are separated by 21 nucleotides GFF6530 and GFF6531 are separated by 138 nucleotides
GFF6528 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 23,564 to 24,241
GFF6528
GFF6529 - Rhodanese-like domain protein, at 24,255 to 24,590
GFF6529
GFF6530 - Type I secretion outer membrane protein, TolC family, at 24,612 to 26,165
GFF6530
GFF6531 - 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12)(EC 2.4.99.13), at 26,304 to 27,689
GFF6531
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.684 kb on + strand, within GFF6528 at 23.684 kb on + strand, within GFF6528 at 23.684 kb on + strand, within GFF6528 at 23.684 kb on + strand, within GFF6528 at 23.684 kb on + strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 23.685 kb on - strand, within GFF6528 at 24.585 kb on + strand at 24.585 kb on + strand at 24.586 kb on - strand at 24.714 kb on + strand at 24.714 kb on + strand at 24.714 kb on + strand at 24.714 kb on + strand at 24.714 kb on + strand at 24.715 kb on - strand at 24.715 kb on - strand at 24.715 kb on - strand at 24.715 kb on - strand at 24.732 kb on + strand at 24.732 kb on + strand at 24.732 kb on + strand at 24.733 kb on - strand at 24.733 kb on - strand at 24.744 kb on + strand at 24.744 kb on + strand at 24.744 kb on + strand at 24.744 kb on + strand at 24.745 kb on - strand at 24.745 kb on - strand at 24.766 kb on - strand at 24.766 kb on - strand at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on + strand, within GFF6530 at 24.972 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 24.973 kb on - strand, within GFF6530 at 25.059 kb on + strand, within GFF6530 at 25.059 kb on + strand, within GFF6530 at 25.059 kb on + strand, within GFF6530 at 25.060 kb on - strand, within GFF6530 at 25.060 kb on - strand, within GFF6530 at 25.060 kb on - strand, within GFF6530 at 25.060 kb on - strand, within GFF6530 at 25.060 kb on - strand, within GFF6530 at 25.203 kb on + strand, within GFF6530 at 25.204 kb on - strand, within GFF6530 at 25.485 kb on + strand, within GFF6530 at 25.485 kb on + strand, within GFF6530 at 25.485 kb on + strand, within GFF6530 at 25.485 kb on + strand, within GFF6530 at 25.486 kb on - strand, within GFF6530 at 25.486 kb on - strand, within GFF6530 at 25.486 kb on - strand, within GFF6530 at 25.695 kb on + strand, within GFF6530 at 25.696 kb on - strand, within GFF6530 at 25.696 kb on - strand, within GFF6530 at 25.789 kb on - strand, within GFF6530 at 25.876 kb on - strand, within GFF6530 at 26.363 kb on + strand at 26.363 kb on + strand at 26.363 kb on + strand at 26.363 kb on + strand at 26.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 23,684 + GFF6528 0.18 -0.1 23,684 + GFF6528 0.18 -0.9 23,684 + GFF6528 0.18 -1.6 23,684 + GFF6528 0.18 -0.1 23,684 + GFF6528 0.18 -1.4 23,685 - GFF6528 0.18 -1.1 23,685 - GFF6528 0.18 -0.5 23,685 - GFF6528 0.18 +0.3 23,685 - GFF6528 0.18 -0.3 23,685 - GFF6528 0.18 -0.4 23,685 - GFF6528 0.18 -1.0 23,685 - GFF6528 0.18 -0.2 23,685 - GFF6528 0.18 +1.1 23,685 - GFF6528 0.18 -0.8 24,585 + -1.2 24,585 + +0.1 24,586 - +0.4 24,714 + +0.1 24,714 + +0.1 24,714 + -0.1 24,714 + -0.8 24,714 + +1.4 24,715 - +1.4 24,715 - -0.4 24,715 - -0.6 24,715 - -0.2 24,732 + -1.5 24,732 + -0.0 24,732 + -0.4 24,733 - -1.9 24,733 - -0.9 24,744 + -0.6 24,744 + -1.4 24,744 + -0.5 24,744 + -0.1 24,745 - +0.5 24,745 - -0.5 24,766 - -1.9 24,766 - +0.2 24,972 + GFF6530 0.23 +0.0 24,972 + GFF6530 0.23 -0.9 24,972 + GFF6530 0.23 +0.4 24,972 + GFF6530 0.23 -0.7 24,972 + GFF6530 0.23 +0.6 24,972 + GFF6530 0.23 -1.1 24,972 + GFF6530 0.23 -1.6 24,972 + GFF6530 0.23 -1.5 24,972 + GFF6530 0.23 +0.5 24,972 - GFF6530 0.23 +0.2 24,973 - GFF6530 0.23 +0.2 24,973 - GFF6530 0.23 -0.4 24,973 - GFF6530 0.23 +0.5 24,973 - GFF6530 0.23 -0.4 24,973 - GFF6530 0.23 +0.4 24,973 - GFF6530 0.23 +0.0 24,973 - GFF6530 0.23 +0.2 24,973 - GFF6530 0.23 -2.4 25,059 + GFF6530 0.29 +0.4 25,059 + GFF6530 0.29 -0.9 25,059 + GFF6530 0.29 +0.1 25,060 - GFF6530 0.29 +0.4 25,060 - GFF6530 0.29 +0.0 25,060 - GFF6530 0.29 -0.6 25,060 - GFF6530 0.29 -2.1 25,060 - GFF6530 0.29 +0.1 25,203 + GFF6530 0.38 -1.5 25,204 - GFF6530 0.38 -0.9 25,485 + GFF6530 0.56 -0.1 25,485 + GFF6530 0.56 -0.4 25,485 + GFF6530 0.56 -0.8 25,485 + GFF6530 0.56 +1.0 25,486 - GFF6530 0.56 +1.1 25,486 - GFF6530 0.56 +0.4 25,486 - GFF6530 0.56 -0.8 25,695 + GFF6530 0.70 +0.1 25,696 - GFF6530 0.70 +2.6 25,696 - GFF6530 0.70 -0.8 25,789 - GFF6530 0.76 -0.1 25,876 - GFF6530 0.81 +0.7 26,363 + -1.8 26,363 + +0.3 26,363 + +0.4 26,363 + -0.4 26,363 + -0.4
Or see this region's nucleotide sequence