Strain Fitness in Variovorax sp. SCN45 around GFF6503

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6503 and GFF6504 overlap by 4 nucleotidesGFF6504 and GFF6505 are separated by 4 nucleotides GFF6503 - no description, at 105 to 368 GFF6503 GFF6504 - no description, at 365 to 1,111 GFF6504 GFF6505 - no description, at 1,116 to 1,529 GFF6505 Position (kb) 0 1Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 0.071 kb on + strandat 0.071 kb on + strandat 0.094 kb on - strandat 0.111 kb on + strandat 0.191 kb on - strand, within GFF6503at 0.191 kb on - strand, within GFF6503at 0.191 kb on - strand, within GFF6503at 0.264 kb on + strand, within GFF6503at 0.264 kb on + strand, within GFF6503at 0.264 kb on + strand, within GFF6503at 0.265 kb on - strand, within GFF6503at 0.265 kb on - strand, within GFF6503at 0.265 kb on - strand, within GFF6503at 0.333 kb on + strand, within GFF6503at 0.333 kb on + strand, within GFF6503at 0.333 kb on + strand, within GFF6503at 0.334 kb on - strand, within GFF6503at 0.334 kb on - strand, within GFF6503at 0.334 kb on - strand, within GFF6503at 0.366 kb on + strandat 0.366 kb on + strandat 0.367 kb on - strandat 0.367 kb on - strandat 0.555 kb on + strand, within GFF6504at 0.556 kb on - strand, within GFF6504at 0.556 kb on - strand, within GFF6504at 0.556 kb on - strand, within GFF6504at 0.657 kb on + strand, within GFF6504at 0.657 kb on + strand, within GFF6504at 0.657 kb on + strand, within GFF6504at 0.658 kb on - strand, within GFF6504at 0.658 kb on - strand, within GFF6504at 0.779 kb on - strand, within GFF6504at 0.795 kb on + strand, within GFF6504at 0.795 kb on + strand, within GFF6504at 0.796 kb on - strand, within GFF6504at 0.796 kb on - strand, within GFF6504at 0.796 kb on - strand, within GFF6504at 0.796 kb on - strand, within GFF6504at 0.796 kb on - strand, within GFF6504at 0.932 kb on + strand, within GFF6504at 0.933 kb on - strand, within GFF6504at 0.987 kb on - strand, within GFF6504at 0.987 kb on - strand, within GFF6504at 0.988 kb on + strand, within GFF6504at 0.988 kb on + strand, within GFF6504at 0.988 kb on + strand, within GFF6504at 0.988 kb on + strand, within GFF6504at 0.988 kb on + strand, within GFF6504at 0.988 kb on + strand, within GFF6504at 0.988 kb on + strand, within GFF6504at 0.989 kb on - strand, within GFF6504at 0.989 kb on - strand, within GFF6504at 0.989 kb on - strand, within GFF6504at 0.989 kb on - strand, within GFF6504at 0.989 kb on - strand, within GFF6504at 0.989 kb on - strand, within GFF6504at 0.994 kb on - strand, within GFF6504at 1.045 kb on + strandat 1.046 kb on - strandat 1.099 kb on + strandat 1.339 kb on - strand, within GFF6505at 1.339 kb on - strand, within GFF6505

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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71 + +0.4
71 + +0.2
94 - -0.3
111 + -0.9
191 - GFF6503 0.33 -0.6
191 - GFF6503 0.33 -1.0
191 - GFF6503 0.33 -0.2
264 + GFF6503 0.60 -0.6
264 + GFF6503 0.60 +0.6
264 + GFF6503 0.60 +2.4
265 - GFF6503 0.61 -0.3
265 - GFF6503 0.61 -0.0
265 - GFF6503 0.61 -0.6
333 + GFF6503 0.86 +0.2
333 + GFF6503 0.86 -2.4
333 + GFF6503 0.86 +0.0
334 - GFF6503 0.87 +1.4
334 - GFF6503 0.87 +1.0
334 - GFF6503 0.87 +0.7
366 + -0.2
366 + -0.8
367 - +0.4
367 - -0.1
555 + GFF6504 0.25 +0.7
556 - GFF6504 0.26 -0.4
556 - GFF6504 0.26 +0.3
556 - GFF6504 0.26 -0.3
657 + GFF6504 0.39 +0.8
657 + GFF6504 0.39 +0.2
657 + GFF6504 0.39 +1.6
658 - GFF6504 0.39 +0.2
658 - GFF6504 0.39 -0.3
779 - GFF6504 0.55 -0.1
795 + GFF6504 0.58 -0.2
795 + GFF6504 0.58 +0.9
796 - GFF6504 0.58 +0.4
796 - GFF6504 0.58 -0.5
796 - GFF6504 0.58 -0.2
796 - GFF6504 0.58 -0.4
796 - GFF6504 0.58 -0.5
932 + GFF6504 0.76 +0.4
933 - GFF6504 0.76 -3.2
987 - GFF6504 0.83 -0.2
987 - GFF6504 0.83 -0.1
988 + GFF6504 0.83 -0.0
988 + GFF6504 0.83 +0.3
988 + GFF6504 0.83 +0.4
988 + GFF6504 0.83 -0.7
988 + GFF6504 0.83 -0.9
988 + GFF6504 0.83 -0.3
988 + GFF6504 0.83 -0.9
989 - GFF6504 0.84 -0.5
989 - GFF6504 0.84 -0.6
989 - GFF6504 0.84 +1.4
989 - GFF6504 0.84 -0.1
989 - GFF6504 0.84 -0.1
989 - GFF6504 0.84 -0.1
994 - GFF6504 0.84 +0.3
1,045 + -2.4
1,046 - -0.3
1,099 + -0.2
1,339 - GFF6505 0.54 -0.5
1,339 - GFF6505 0.54 -0.8

Or see this region's nucleotide sequence