Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6389 and GFF6390 are separated by 9 nucleotides GFF6390 and GFF6391 overlap by 4 nucleotides GFF6391 and GFF6392 overlap by 4 nucleotides
GFF6389 - Amidohydrolase domain protein, at 61,667 to 62,866
GFF6389
GFF6390 - GtrA-like protein, at 62,876 to 63,277
GFF6390
GFF6391 - RfbJ protein, at 63,274 to 64,242
GFF6391
GFF6392 - hypothetical protein, at 64,239 to 66,074
GFF6392
Position (kb) 62
63
64 Strain fitness (log2 ratio) -2
-1
0
1
2 at 62.213 kb on + strand, within GFF6389 at 62.249 kb on + strand, within GFF6389 at 62.250 kb on - strand, within GFF6389 at 62.368 kb on + strand, within GFF6389 at 62.369 kb on - strand, within GFF6389 at 62.369 kb on - strand, within GFF6389 at 62.369 kb on - strand, within GFF6389 at 62.908 kb on - strand at 62.908 kb on - strand at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.018 kb on + strand, within GFF6390 at 63.018 kb on + strand, within GFF6390 at 63.019 kb on - strand, within GFF6390 at 63.019 kb on - strand, within GFF6390 at 63.055 kb on - strand, within GFF6390 at 63.055 kb on - strand, within GFF6390 at 63.063 kb on + strand, within GFF6390 at 63.063 kb on + strand, within GFF6390 at 63.064 kb on - strand, within GFF6390 at 63.064 kb on - strand, within GFF6390 at 63.111 kb on + strand, within GFF6390 at 63.111 kb on + strand, within GFF6390 at 63.111 kb on + strand, within GFF6390 at 63.112 kb on - strand, within GFF6390 at 63.112 kb on - strand, within GFF6390 at 63.112 kb on - strand, within GFF6390 at 63.175 kb on - strand, within GFF6390 at 63.202 kb on - strand, within GFF6390 at 63.202 kb on - strand, within GFF6390 at 63.202 kb on - strand, within GFF6390 at 63.314 kb on + strand at 63.314 kb on + strand at 63.314 kb on + strand at 63.315 kb on - strand at 63.315 kb on - strand at 63.315 kb on - strand at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.514 kb on + strand, within GFF6391 at 63.591 kb on - strand, within GFF6391 at 63.632 kb on + strand, within GFF6391 at 63.632 kb on + strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.653 kb on + strand, within GFF6391 at 63.653 kb on + strand, within GFF6391 at 63.654 kb on - strand, within GFF6391 at 63.848 kb on + strand, within GFF6391 at 63.950 kb on + strand, within GFF6391 at 63.951 kb on - strand, within GFF6391 at 63.951 kb on - strand, within GFF6391 at 63.977 kb on + strand, within GFF6391 at 63.977 kb on + strand, within GFF6391 at 63.978 kb on - strand, within GFF6391 at 63.978 kb on - strand, within GFF6391 at 64.181 kb on + strand at 64.181 kb on + strand at 64.181 kb on + strand at 64.181 kb on + strand at 64.181 kb on + strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.260 kb on - strand at 64.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 62,213 + GFF6389 0.46 -0.5 62,249 + GFF6389 0.48 -0.5 62,250 - GFF6389 0.49 -0.9 62,368 + GFF6389 0.58 -0.0 62,369 - GFF6389 0.58 -0.1 62,369 - GFF6389 0.58 +0.1 62,369 - GFF6389 0.58 -0.2 62,908 - -1.7 62,908 - -0.1 63,009 + GFF6390 0.33 -1.4 63,009 + GFF6390 0.33 +0.6 63,009 + GFF6390 0.33 +1.5 63,009 + GFF6390 0.33 -0.3 63,009 + GFF6390 0.33 -2.1 63,009 + GFF6390 0.33 -1.4 63,009 + GFF6390 0.33 -1.0 63,009 + GFF6390 0.33 -0.2 63,010 - GFF6390 0.33 +0.5 63,010 - GFF6390 0.33 -1.4 63,010 - GFF6390 0.33 +1.2 63,010 - GFF6390 0.33 +1.0 63,018 + GFF6390 0.35 -0.7 63,018 + GFF6390 0.35 +0.5 63,019 - GFF6390 0.36 -1.0 63,019 - GFF6390 0.36 +0.0 63,055 - GFF6390 0.45 -0.4 63,055 - GFF6390 0.45 -0.9 63,063 + GFF6390 0.47 -0.1 63,063 + GFF6390 0.47 -0.5 63,064 - GFF6390 0.47 -0.6 63,064 - GFF6390 0.47 -0.8 63,111 + GFF6390 0.58 -0.2 63,111 + GFF6390 0.58 -0.0 63,111 + GFF6390 0.58 -2.4 63,112 - GFF6390 0.59 -0.4 63,112 - GFF6390 0.59 -0.4 63,112 - GFF6390 0.59 -0.1 63,175 - GFF6390 0.74 +0.5 63,202 - GFF6390 0.81 +1.4 63,202 - GFF6390 0.81 +0.3 63,202 - GFF6390 0.81 -1.0 63,314 + +0.8 63,314 + +0.0 63,314 + +0.0 63,315 - -0.2 63,315 - -0.3 63,315 - +0.4 63,455 + GFF6391 0.19 -0.2 63,455 + GFF6391 0.19 +1.0 63,455 + GFF6391 0.19 -0.3 63,455 + GFF6391 0.19 -1.4 63,455 + GFF6391 0.19 +0.2 63,455 + GFF6391 0.19 -0.2 63,455 + GFF6391 0.19 -0.9 63,456 - GFF6391 0.19 +0.2 63,456 - GFF6391 0.19 +1.8 63,456 - GFF6391 0.19 +0.9 63,456 - GFF6391 0.19 +0.1 63,456 - GFF6391 0.19 -1.0 63,456 - GFF6391 0.19 -0.2 63,456 - GFF6391 0.19 +1.2 63,456 - GFF6391 0.19 +0.3 63,456 - GFF6391 0.19 -1.5 63,456 - GFF6391 0.19 -0.6 63,514 + GFF6391 0.25 +0.4 63,591 - GFF6391 0.33 -1.0 63,632 + GFF6391 0.37 -0.3 63,632 + GFF6391 0.37 -0.6 63,633 - GFF6391 0.37 +1.5 63,633 - GFF6391 0.37 -0.6 63,633 - GFF6391 0.37 -0.3 63,633 - GFF6391 0.37 +0.0 63,633 - GFF6391 0.37 +0.1 63,653 + GFF6391 0.39 -0.4 63,653 + GFF6391 0.39 -0.2 63,654 - GFF6391 0.39 -1.0 63,848 + GFF6391 0.59 -0.6 63,950 + GFF6391 0.70 -0.2 63,951 - GFF6391 0.70 -0.8 63,951 - GFF6391 0.70 -0.5 63,977 + GFF6391 0.73 +0.2 63,977 + GFF6391 0.73 -0.5 63,978 - GFF6391 0.73 -0.4 63,978 - GFF6391 0.73 +0.1 64,181 + +0.6 64,181 + +2.5 64,181 + -0.2 64,181 + +1.0 64,181 + -1.2 64,182 - +0.4 64,182 - -0.2 64,182 - -0.4 64,182 - +1.1 64,182 - -0.1 64,182 - +0.0 64,182 - +0.1 64,182 - -0.9 64,182 - +0.8 64,260 - -0.2 64,264 + -0.2
Or see this region's nucleotide sequence