Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6251 and GFF6252 are separated by 10 nucleotides GFF6252 and GFF6253 are separated by 31 nucleotides GFF6253 and GFF6254 are separated by 55 nucleotides
GFF6251 - hypothetical protein, at 45,450 to 46,019
GFF6251
GFF6252 - Phosphonate ABC transporter permease protein PhnE (TC 3.A.1.9.1), at 46,030 to 46,833
GFF6252
GFF6253 - Phosphonate ABC transporter substrate-binding protein PhnD (TC 3.A.1.9.1), at 46,865 to 47,815
GFF6253
GFF6254 - Phosphonate ABC transporter ATP-binding protein PhnC (TC 3.A.1.9.1), at 47,871 to 48,728
GFF6254
Position (kb) 46
47
48 Strain fitness (log2 ratio) -1
0
1
2 at 46.031 kb on + strand at 46.031 kb on + strand at 46.032 kb on - strand at 46.032 kb on - strand at 46.616 kb on + strand, within GFF6252 at 46.616 kb on + strand, within GFF6252 at 46.617 kb on - strand, within GFF6252 at 46.617 kb on - strand, within GFF6252 at 47.076 kb on + strand, within GFF6253 at 47.077 kb on - strand, within GFF6253 at 47.352 kb on + strand, within GFF6253 at 47.481 kb on + strand, within GFF6253 at 47.482 kb on - strand, within GFF6253 at 47.484 kb on + strand, within GFF6253 at 47.484 kb on + strand, within GFF6253 at 47.485 kb on - strand, within GFF6253 at 47.485 kb on - strand, within GFF6253 at 47.485 kb on - strand, within GFF6253 at 47.623 kb on - strand, within GFF6253 at 47.623 kb on - strand, within GFF6253 at 47.980 kb on + strand, within GFF6254 at 47.981 kb on - strand, within GFF6254 at 48.070 kb on + strand, within GFF6254 at 48.070 kb on + strand, within GFF6254 at 48.071 kb on - strand, within GFF6254
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 46,031 + -1.2 46,031 + -0.1 46,032 - -0.4 46,032 - -1.3 46,616 + GFF6252 0.73 +0.8 46,616 + GFF6252 0.73 +0.1 46,617 - GFF6252 0.73 -0.6 46,617 - GFF6252 0.73 -1.3 47,076 + GFF6253 0.22 -0.2 47,077 - GFF6253 0.22 -0.7 47,352 + GFF6253 0.51 +0.0 47,481 + GFF6253 0.65 +0.1 47,482 - GFF6253 0.65 -0.3 47,484 + GFF6253 0.65 +0.6 47,484 + GFF6253 0.65 -1.3 47,485 - GFF6253 0.65 +2.5 47,485 - GFF6253 0.65 -0.9 47,485 - GFF6253 0.65 +0.3 47,623 - GFF6253 0.80 +0.5 47,623 - GFF6253 0.80 -1.2 47,980 + GFF6254 0.13 -0.2 47,981 - GFF6254 0.13 +0.2 48,070 + GFF6254 0.23 -0.6 48,070 + GFF6254 0.23 -0.1 48,071 - GFF6254 0.23 +0.1
Or see this region's nucleotide sequence