Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6233 and GFF6234 are separated by 1 nucleotides GFF6234 and GFF6235 overlap by 4 nucleotides GFF6235 and GFF6236 are separated by 390 nucleotides
GFF6233 - hypothetical protein, at 21,724 to 23,433
GFF6233
GFF6234 - hypothetical protein, at 23,435 to 23,584
GFF6234
GFF6235 - hypothetical protein, at 23,581 to 25,701
GFF6235
GFF6236 - VgrG protein, at 26,092 to 28,746
GFF6236
Position (kb) 23
24
25
26 Strain fitness (log2 ratio) -2
-1
0
1 at 22.586 kb on + strand, within GFF6233 at 22.587 kb on - strand, within GFF6233 at 22.698 kb on - strand, within GFF6233 at 22.721 kb on + strand, within GFF6233 at 22.721 kb on + strand, within GFF6233 at 22.722 kb on - strand, within GFF6233 at 23.133 kb on - strand, within GFF6233 at 23.133 kb on - strand, within GFF6233 at 23.407 kb on - strand at 23.483 kb on - strand, within GFF6234 at 23.601 kb on + strand at 23.601 kb on + strand at 23.602 kb on - strand at 23.704 kb on + strand at 23.704 kb on + strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.984 kb on + strand, within GFF6235 at 23.985 kb on - strand, within GFF6235 at 23.985 kb on - strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.097 kb on - strand, within GFF6235 at 24.097 kb on - strand, within GFF6235 at 24.155 kb on + strand, within GFF6235 at 24.155 kb on + strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.282 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235 at 24.728 kb on + strand, within GFF6235 at 24.729 kb on - strand, within GFF6235 at 24.917 kb on + strand, within GFF6235 at 24.918 kb on - strand, within GFF6235 at 24.918 kb on - strand, within GFF6235 at 24.920 kb on + strand, within GFF6235 at 24.920 kb on + strand, within GFF6235 at 24.920 kb on + strand, within GFF6235 at 24.921 kb on - strand, within GFF6235 at 24.921 kb on - strand, within GFF6235 at 24.921 kb on - strand, within GFF6235 at 24.921 kb on - strand, within GFF6235 at 24.921 kb on - strand, within GFF6235 at 24.921 kb on - strand, within GFF6235 at 24.921 kb on - strand, within GFF6235 at 25.146 kb on - strand, within GFF6235 at 25.178 kb on + strand, within GFF6235 at 25.178 kb on + strand, within GFF6235 at 25.179 kb on - strand, within GFF6235 at 25.179 kb on - strand, within GFF6235 at 25.179 kb on - strand, within GFF6235 at 25.236 kb on + strand, within GFF6235 at 25.236 kb on + strand, within GFF6235 at 25.237 kb on - strand, within GFF6235 at 25.237 kb on - strand, within GFF6235 at 25.328 kb on + strand, within GFF6235 at 25.328 kb on + strand, within GFF6235 at 25.329 kb on - strand, within GFF6235 at 25.335 kb on - strand, within GFF6235 at 25.335 kb on - strand, within GFF6235 at 25.475 kb on + strand, within GFF6235 at 25.475 kb on + strand, within GFF6235 at 25.511 kb on + strand at 25.850 kb on + strand at 25.883 kb on - strand at 25.883 kb on - strand at 25.889 kb on + strand at 25.895 kb on + strand at 26.025 kb on - strand at 26.035 kb on - strand at 26.344 kb on + strand at 26.344 kb on + strand at 26.345 kb on - strand at 26.345 kb on - strand at 26.345 kb on - strand at 26.345 kb on - strand at 26.488 kb on + strand, within GFF6236 at 26.489 kb on - strand, within GFF6236 at 26.489 kb on - strand, within GFF6236 at 26.503 kb on + strand, within GFF6236 at 26.503 kb on + strand, within GFF6236 at 26.504 kb on - strand, within GFF6236 at 26.515 kb on + strand, within GFF6236 at 26.549 kb on + strand, within GFF6236 at 26.550 kb on - strand, within GFF6236
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 22,586 + GFF6233 0.50 -0.8 22,587 - GFF6233 0.50 -0.6 22,698 - GFF6233 0.57 -0.1 22,721 + GFF6233 0.58 +0.1 22,721 + GFF6233 0.58 +0.7 22,722 - GFF6233 0.58 -0.4 23,133 - GFF6233 0.82 -0.7 23,133 - GFF6233 0.82 -0.0 23,407 - -0.3 23,483 - GFF6234 0.32 +1.3 23,601 + -0.4 23,601 + +0.5 23,602 - +0.4 23,704 + -0.3 23,704 + -0.7 23,705 - -1.5 23,705 - -1.6 23,705 - -0.6 23,705 - -0.7 23,705 - +0.0 23,984 + GFF6235 0.19 +1.0 23,985 - GFF6235 0.19 +0.7 23,985 - GFF6235 0.19 -0.6 24,089 + GFF6235 0.24 -0.0 24,089 + GFF6235 0.24 +0.6 24,089 + GFF6235 0.24 -0.2 24,089 + GFF6235 0.24 +1.3 24,089 + GFF6235 0.24 -1.4 24,089 + GFF6235 0.24 +0.7 24,089 + GFF6235 0.24 +0.4 24,089 + GFF6235 0.24 -0.3 24,090 - GFF6235 0.24 +0.8 24,090 - GFF6235 0.24 -0.7 24,090 - GFF6235 0.24 +1.3 24,090 - GFF6235 0.24 -0.3 24,090 - GFF6235 0.24 -0.6 24,097 - GFF6235 0.24 -0.9 24,097 - GFF6235 0.24 -1.4 24,155 + GFF6235 0.27 -0.3 24,155 + GFF6235 0.27 -0.3 24,156 - GFF6235 0.27 -0.2 24,156 - GFF6235 0.27 +0.9 24,156 - GFF6235 0.27 -1.9 24,282 - GFF6235 0.33 -0.5 24,318 - GFF6235 0.35 +0.1 24,318 - GFF6235 0.35 +0.6 24,318 - GFF6235 0.35 -0.2 24,728 + GFF6235 0.54 -1.3 24,729 - GFF6235 0.54 -0.2 24,917 + GFF6235 0.63 -0.8 24,918 - GFF6235 0.63 -0.4 24,918 - GFF6235 0.63 -1.0 24,920 + GFF6235 0.63 +0.3 24,920 + GFF6235 0.63 -0.5 24,920 + GFF6235 0.63 +1.1 24,921 - GFF6235 0.63 -0.9 24,921 - GFF6235 0.63 -0.4 24,921 - GFF6235 0.63 +1.6 24,921 - GFF6235 0.63 -0.5 24,921 - GFF6235 0.63 +0.6 24,921 - GFF6235 0.63 +0.8 24,921 - GFF6235 0.63 -0.0 25,146 - GFF6235 0.74 +0.1 25,178 + GFF6235 0.75 +1.0 25,178 + GFF6235 0.75 +0.9 25,179 - GFF6235 0.75 +0.9 25,179 - GFF6235 0.75 +1.7 25,179 - GFF6235 0.75 -0.2 25,236 + GFF6235 0.78 -0.3 25,236 + GFF6235 0.78 +0.0 25,237 - GFF6235 0.78 -0.8 25,237 - GFF6235 0.78 -0.9 25,328 + GFF6235 0.82 -0.0 25,328 + GFF6235 0.82 +0.3 25,329 - GFF6235 0.82 +0.6 25,335 - GFF6235 0.83 -0.1 25,335 - GFF6235 0.83 -1.2 25,475 + GFF6235 0.89 -0.7 25,475 + GFF6235 0.89 -0.3 25,511 + +0.4 25,850 + -0.3 25,883 - +0.3 25,883 - -0.9 25,889 + -0.0 25,895 + +0.3 26,025 - +0.0 26,035 - -0.5 26,344 + -0.3 26,344 + -0.5 26,345 - +0.6 26,345 - -0.4 26,345 - +0.7 26,345 - -1.1 26,488 + GFF6236 0.15 -1.5 26,489 - GFF6236 0.15 +0.5 26,489 - GFF6236 0.15 +0.0 26,503 + GFF6236 0.15 -1.1 26,503 + GFF6236 0.15 -0.5 26,504 - GFF6236 0.16 -0.5 26,515 + GFF6236 0.16 +1.7 26,549 + GFF6236 0.17 +0.3 26,550 - GFF6236 0.17 -1.7
Or see this region's nucleotide sequence