Strain Fitness in Variovorax sp. SCN45 around GFF6002

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6001 and GFF6002 are separated by 105 nucleotidesGFF6002 and GFF6003 are separated by 62 nucleotidesGFF6003 and GFF6004 are separated by 66 nucleotides GFF6001 - Purine nucleoside permease, at 59,400 to 60,455 GFF6001 GFF6002 - DNA topoisomerase IB (poxvirus type) (EC 5.99.1.2), at 60,561 to 61,811 GFF6002 GFF6003 - no description, at 61,874 to 62,488 GFF6003 GFF6004 - hypothetical protein, at 62,555 to 62,734 GFF6004 Position (kb) 60 61 62Strain fitness (log2 ratio) -2 -1 0 1 2at 59.668 kb on + strand, within GFF6001at 59.668 kb on + strand, within GFF6001at 59.668 kb on + strand, within GFF6001at 59.669 kb on - strand, within GFF6001at 59.683 kb on + strand, within GFF6001at 59.684 kb on - strand, within GFF6001at 59.698 kb on + strand, within GFF6001at 59.698 kb on + strand, within GFF6001at 59.698 kb on + strand, within GFF6001at 59.698 kb on + strand, within GFF6001at 59.698 kb on + strand, within GFF6001at 59.698 kb on + strand, within GFF6001at 59.699 kb on - strand, within GFF6001at 59.699 kb on - strand, within GFF6001at 59.699 kb on - strand, within GFF6001at 59.699 kb on - strand, within GFF6001at 59.750 kb on + strand, within GFF6001at 59.751 kb on - strand, within GFF6001at 59.751 kb on - strand, within GFF6001at 59.751 kb on - strand, within GFF6001at 59.751 kb on - strand, within GFF6001at 59.915 kb on - strand, within GFF6001at 59.987 kb on - strand, within GFF6001at 59.987 kb on - strand, within GFF6001at 60.067 kb on + strand, within GFF6001at 60.067 kb on + strand, within GFF6001at 60.067 kb on + strand, within GFF6001at 60.068 kb on - strand, within GFF6001at 60.068 kb on - strand, within GFF6001at 60.068 kb on - strand, within GFF6001at 60.068 kb on - strand, within GFF6001at 60.070 kb on + strand, within GFF6001at 60.070 kb on + strand, within GFF6001at 60.070 kb on + strand, within GFF6001at 60.070 kb on + strand, within GFF6001at 60.070 kb on + strand, within GFF6001at 60.071 kb on - strand, within GFF6001at 60.071 kb on - strand, within GFF6001at 60.071 kb on - strand, within GFF6001at 60.071 kb on - strand, within GFF6001at 60.071 kb on - strand, within GFF6001at 60.181 kb on + strand, within GFF6001at 60.182 kb on - strand, within GFF6001at 60.182 kb on - strand, within GFF6001at 60.182 kb on - strand, within GFF6001at 60.182 kb on - strand, within GFF6001at 60.233 kb on - strand, within GFF6001at 60.242 kb on - strand, within GFF6001at 60.505 kb on + strandat 60.506 kb on - strandat 60.506 kb on - strandat 60.543 kb on + strandat 60.544 kb on - strandat 60.544 kb on - strandat 60.654 kb on + strandat 60.654 kb on + strandat 60.654 kb on + strandat 60.790 kb on - strand, within GFF6002at 60.790 kb on - strand, within GFF6002at 60.790 kb on - strand, within GFF6002at 60.790 kb on - strand, within GFF6002at 60.790 kb on - strand, within GFF6002at 60.874 kb on - strand, within GFF6002at 61.110 kb on + strand, within GFF6002at 61.279 kb on - strand, within GFF6002at 61.332 kb on + strand, within GFF6002at 61.332 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.536 kb on + strand, within GFF6002at 61.537 kb on - strand, within GFF6002at 61.537 kb on - strand, within GFF6002at 61.537 kb on - strand, within GFF6002at 61.537 kb on - strand, within GFF6002at 61.537 kb on - strand, within GFF6002at 61.537 kb on - strand, within GFF6002at 61.656 kb on + strand, within GFF6002at 61.657 kb on - strand, within GFF6002at 61.657 kb on - strand, within GFF6002at 61.657 kb on - strand, within GFF6002at 61.809 kb on + strandat 62.019 kb on + strand, within GFF6003at 62.020 kb on - strand, within GFF6003at 62.020 kb on - strand, within GFF6003at 62.479 kb on + strandat 62.479 kb on + strandat 62.508 kb on + strandat 62.508 kb on + strandat 62.508 kb on + strandat 62.509 kb on - strandat 62.509 kb on - strandat 62.509 kb on - strandat 62.509 kb on - strandat 62.509 kb on - strandat 62.535 kb on + strandat 62.536 kb on - strandat 62.536 kb on - strandat 62.548 kb on - strandat 62.571 kb on + strandat 62.571 kb on + strandat 62.572 kb on - strandat 62.572 kb on - strandat 62.658 kb on + strand, within GFF6004

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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59,668 + GFF6001 0.25 +0.3
59,668 + GFF6001 0.25 +0.5
59,668 + GFF6001 0.25 -0.8
59,669 - GFF6001 0.25 +0.2
59,683 + GFF6001 0.27 +2.6
59,684 - GFF6001 0.27 +0.2
59,698 + GFF6001 0.28 -2.2
59,698 + GFF6001 0.28 -0.8
59,698 + GFF6001 0.28 +0.6
59,698 + GFF6001 0.28 -0.8
59,698 + GFF6001 0.28 -0.2
59,698 + GFF6001 0.28 -0.5
59,699 - GFF6001 0.28 +0.2
59,699 - GFF6001 0.28 -0.1
59,699 - GFF6001 0.28 -0.1
59,699 - GFF6001 0.28 -1.0
59,750 + GFF6001 0.33 +1.1
59,751 - GFF6001 0.33 -0.4
59,751 - GFF6001 0.33 -0.6
59,751 - GFF6001 0.33 -0.2
59,751 - GFF6001 0.33 -1.1
59,915 - GFF6001 0.49 +0.0
59,987 - GFF6001 0.56 -0.1
59,987 - GFF6001 0.56 -0.0
60,067 + GFF6001 0.63 +0.1
60,067 + GFF6001 0.63 +0.1
60,067 + GFF6001 0.63 -0.2
60,068 - GFF6001 0.63 -0.4
60,068 - GFF6001 0.63 +0.4
60,068 - GFF6001 0.63 -0.3
60,068 - GFF6001 0.63 +0.1
60,070 + GFF6001 0.63 -0.2
60,070 + GFF6001 0.63 -1.2
60,070 + GFF6001 0.63 +0.8
60,070 + GFF6001 0.63 +1.6
60,070 + GFF6001 0.63 -0.4
60,071 - GFF6001 0.64 -0.6
60,071 - GFF6001 0.64 -1.2
60,071 - GFF6001 0.64 -0.2
60,071 - GFF6001 0.64 -0.0
60,071 - GFF6001 0.64 +0.8
60,181 + GFF6001 0.74 -0.4
60,182 - GFF6001 0.74 -0.1
60,182 - GFF6001 0.74 -0.0
60,182 - GFF6001 0.74 -0.0
60,182 - GFF6001 0.74 -0.8
60,233 - GFF6001 0.79 +0.2
60,242 - GFF6001 0.80 -0.0
60,505 + -1.6
60,506 - -0.6
60,506 - -0.3
60,543 + +1.8
60,544 - -0.1
60,544 - -0.1
60,654 + -0.5
60,654 + -0.7
60,654 + -2.2
60,790 - GFF6002 0.18 +0.0
60,790 - GFF6002 0.18 -0.0
60,790 - GFF6002 0.18 +0.5
60,790 - GFF6002 0.18 +0.2
60,790 - GFF6002 0.18 -1.4
60,874 - GFF6002 0.25 -0.4
61,110 + GFF6002 0.44 -0.3
61,279 - GFF6002 0.57 -0.5
61,332 + GFF6002 0.62 -0.0
61,332 + GFF6002 0.62 +1.1
61,536 + GFF6002 0.78 -0.4
61,536 + GFF6002 0.78 +0.1
61,536 + GFF6002 0.78 -0.9
61,536 + GFF6002 0.78 -0.5
61,536 + GFF6002 0.78 -0.0
61,536 + GFF6002 0.78 +0.6
61,536 + GFF6002 0.78 -0.8
61,536 + GFF6002 0.78 -1.1
61,536 + GFF6002 0.78 +0.1
61,537 - GFF6002 0.78 -0.4
61,537 - GFF6002 0.78 +0.7
61,537 - GFF6002 0.78 -1.6
61,537 - GFF6002 0.78 -0.3
61,537 - GFF6002 0.78 +0.2
61,537 - GFF6002 0.78 -0.4
61,656 + GFF6002 0.88 -0.3
61,657 - GFF6002 0.88 +0.8
61,657 - GFF6002 0.88 -1.6
61,657 - GFF6002 0.88 -0.6
61,809 + -0.1
62,019 + GFF6003 0.24 -0.2
62,020 - GFF6003 0.24 -0.6
62,020 - GFF6003 0.24 -0.4
62,479 + -0.0
62,479 + -0.9
62,508 + -0.2
62,508 + +0.1
62,508 + -1.9
62,509 - -0.7
62,509 - -0.6
62,509 - +0.9
62,509 - -0.5
62,509 - +1.9
62,535 + +0.1
62,536 - +1.6
62,536 - -1.5
62,548 - +0.8
62,571 + +0.4
62,571 + -0.5
62,572 - -0.3
62,572 - -1.6
62,658 + GFF6004 0.57 +0.8

Or see this region's nucleotide sequence