Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5509 and GFF5510 are separated by 0 nucleotides GFF5510 and GFF5511 are separated by 135 nucleotides GFF5511 and GFF5512 are separated by 36 nucleotides GFF5512 and GFF5513 overlap by 4 nucleotides
GFF5509 - Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 140,352 to 141,257
GFF5509
GFF5510 - protein of unknown function DUF306, Meta and HslJ, at 141,258 to 141,713
GFF5510
GFF5511 - FIG000875: Thioredoxin domain-containing protein EC-YbbN, at 141,849 to 142,769
GFF5511
GFF5512 - N5-carboxyaminoimidazole ribonucleotide mutase (EC 5.4.99.18), at 142,806 to 143,360
GFF5512
GFF5513 - N5-carboxyaminoimidazole ribonucleotide synthase (EC 6.3.4.18), at 143,357 to 144,547
GFF5513
Position (kb) 141
142
143 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 140.850 kb on + strand, within GFF5509 at 140.850 kb on + strand, within GFF5509 at 140.850 kb on + strand, within GFF5509 at 140.850 kb on + strand, within GFF5509 at 140.850 kb on + strand, within GFF5509 at 140.850 kb on + strand, within GFF5509 at 140.851 kb on - strand, within GFF5509 at 140.851 kb on - strand, within GFF5509 at 140.919 kb on + strand, within GFF5509 at 140.920 kb on - strand, within GFF5509 at 140.920 kb on - strand, within GFF5509 at 140.920 kb on - strand, within GFF5509 at 140.920 kb on - strand, within GFF5509 at 140.920 kb on - strand, within GFF5509 at 141.048 kb on + strand, within GFF5509 at 141.048 kb on + strand, within GFF5509 at 141.048 kb on + strand, within GFF5509 at 141.049 kb on - strand, within GFF5509 at 141.066 kb on + strand, within GFF5509 at 141.066 kb on + strand, within GFF5509 at 141.066 kb on + strand, within GFF5509 at 141.066 kb on + strand, within GFF5509 at 141.067 kb on - strand, within GFF5509 at 141.067 kb on - strand, within GFF5509 at 141.105 kb on + strand, within GFF5509 at 141.105 kb on + strand, within GFF5509 at 141.106 kb on - strand, within GFF5509 at 141.106 kb on - strand, within GFF5509 at 141.225 kb on + strand at 141.225 kb on + strand at 141.225 kb on + strand at 141.225 kb on + strand at 141.226 kb on - strand at 141.226 kb on - strand at 141.226 kb on - strand at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.343 kb on + strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.344 kb on - strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.466 kb on + strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.467 kb on - strand, within GFF5510 at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on + strand at 141.765 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.766 kb on - strand at 141.883 kb on + strand at 141.883 kb on + strand at 141.883 kb on + strand at 141.883 kb on + strand at 141.883 kb on + strand at 141.883 kb on + strand at 141.884 kb on - strand at 141.884 kb on - strand at 141.884 kb on - strand at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.970 kb on + strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 141.971 kb on - strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.297 kb on + strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.298 kb on - strand, within GFF5511 at 142.627 kb on + strand, within GFF5511 at 142.627 kb on + strand, within GFF5511 at 142.628 kb on - strand, within GFF5511 at 142.628 kb on - strand, within GFF5511 at 142.793 kb on + strand at 142.793 kb on + strand at 142.794 kb on - strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.956 kb on + strand, within GFF5512 at 142.957 kb on - strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.110 kb on - strand, within GFF5512 at 143.110 kb on - strand, within GFF5512 at 143.459 kb on + strand at 143.459 kb on + strand at 143.534 kb on + strand, within GFF5513 at 143.534 kb on + strand, within GFF5513
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 140,850 + GFF5509 0.55 -0.8 140,850 + GFF5509 0.55 -0.5 140,850 + GFF5509 0.55 -1.7 140,850 + GFF5509 0.55 +0.8 140,850 + GFF5509 0.55 -0.5 140,850 + GFF5509 0.55 -0.5 140,851 - GFF5509 0.55 -1.9 140,851 - GFF5509 0.55 +0.8 140,919 + GFF5509 0.63 -0.5 140,920 - GFF5509 0.63 +1.8 140,920 - GFF5509 0.63 -2.1 140,920 - GFF5509 0.63 -0.5 140,920 - GFF5509 0.63 -1.4 140,920 - GFF5509 0.63 +0.8 141,048 + GFF5509 0.77 -0.2 141,048 + GFF5509 0.77 -0.5 141,048 + GFF5509 0.77 -0.5 141,049 - GFF5509 0.77 -0.5 141,066 + GFF5509 0.79 +1.8 141,066 + GFF5509 0.79 -1.2 141,066 + GFF5509 0.79 +0.2 141,066 + GFF5509 0.79 +0.8 141,067 - GFF5509 0.79 -0.8 141,067 - GFF5509 0.79 +0.2 141,105 + GFF5509 0.83 +0.2 141,105 + GFF5509 0.83 -3.5 141,106 - GFF5509 0.83 -1.7 141,106 - GFF5509 0.83 -1.4 141,225 + -0.8 141,225 + -0.8 141,225 + -0.8 141,225 + -0.2 141,226 - -0.2 141,226 - -0.2 141,226 - +1.2 141,343 + GFF5510 0.19 +0.3 141,343 + GFF5510 0.19 -0.3 141,343 + GFF5510 0.19 +1.1 141,343 + GFF5510 0.19 -1.3 141,343 + GFF5510 0.19 -0.4 141,343 + GFF5510 0.19 -1.3 141,343 + GFF5510 0.19 -0.6 141,343 + GFF5510 0.19 -1.5 141,343 + GFF5510 0.19 -0.4 141,343 + GFF5510 0.19 +0.0 141,343 + GFF5510 0.19 +0.5 141,343 + GFF5510 0.19 -0.2 141,344 - GFF5510 0.19 -0.0 141,344 - GFF5510 0.19 +0.9 141,344 - GFF5510 0.19 +0.8 141,344 - GFF5510 0.19 +0.1 141,344 - GFF5510 0.19 +0.6 141,344 - GFF5510 0.19 +1.8 141,344 - GFF5510 0.19 -0.7 141,344 - GFF5510 0.19 -1.2 141,344 - GFF5510 0.19 +0.2 141,344 - GFF5510 0.19 +2.4 141,344 - GFF5510 0.19 -0.1 141,344 - GFF5510 0.19 -1.0 141,344 - GFF5510 0.19 -0.1 141,466 + GFF5510 0.46 +0.8 141,466 + GFF5510 0.46 +0.5 141,466 + GFF5510 0.46 +0.2 141,466 + GFF5510 0.46 -1.2 141,466 + GFF5510 0.46 +0.6 141,466 + GFF5510 0.46 -0.6 141,466 + GFF5510 0.46 -0.3 141,466 + GFF5510 0.46 -2.1 141,466 + GFF5510 0.46 +0.8 141,466 + GFF5510 0.46 +0.1 141,466 + GFF5510 0.46 -0.2 141,466 + GFF5510 0.46 -0.7 141,466 + GFF5510 0.46 -0.3 141,467 - GFF5510 0.46 -0.6 141,467 - GFF5510 0.46 +0.1 141,467 - GFF5510 0.46 -0.5 141,467 - GFF5510 0.46 -0.5 141,467 - GFF5510 0.46 -0.7 141,467 - GFF5510 0.46 -0.5 141,467 - GFF5510 0.46 -0.7 141,467 - GFF5510 0.46 -0.8 141,467 - GFF5510 0.46 -0.7 141,467 - GFF5510 0.46 -0.5 141,467 - GFF5510 0.46 -0.0 141,467 - GFF5510 0.46 -0.9 141,467 - GFF5510 0.46 -0.1 141,467 - GFF5510 0.46 -0.5 141,467 - GFF5510 0.46 +0.8 141,467 - GFF5510 0.46 -0.4 141,467 - GFF5510 0.46 +1.3 141,467 - GFF5510 0.46 -0.0 141,467 - GFF5510 0.46 -0.3 141,467 - GFF5510 0.46 -1.5 141,467 - GFF5510 0.46 -0.8 141,467 - GFF5510 0.46 -1.3 141,467 - GFF5510 0.46 -1.1 141,467 - GFF5510 0.46 +0.3 141,467 - GFF5510 0.46 -0.0 141,467 - GFF5510 0.46 +0.4 141,765 + +0.1 141,765 + +0.2 141,765 + -0.6 141,765 + -0.3 141,765 + +1.2 141,765 + +0.2 141,765 + -0.9 141,765 + +0.6 141,765 + +0.3 141,765 + +0.6 141,765 + -0.2 141,765 + +0.3 141,765 + -0.9 141,765 + +0.8 141,765 + +0.7 141,765 - +0.4 141,766 - +0.4 141,766 - -1.3 141,766 - +0.0 141,766 - +0.6 141,766 - +0.1 141,766 - -0.4 141,766 - -1.1 141,766 - -0.5 141,766 - +0.1 141,766 - -0.7 141,766 - -0.3 141,766 - -1.0 141,883 + -0.5 141,883 + -0.6 141,883 + -0.2 141,883 + +2.5 141,883 + +0.3 141,883 + -2.1 141,884 - -0.5 141,884 - -0.6 141,884 - -0.4 141,970 + GFF5511 0.13 -1.0 141,970 + GFF5511 0.13 +0.1 141,970 + GFF5511 0.13 -1.0 141,970 + GFF5511 0.13 +0.2 141,970 + GFF5511 0.13 +0.6 141,970 + GFF5511 0.13 -1.2 141,970 + GFF5511 0.13 -1.0 141,970 + GFF5511 0.13 -0.2 141,970 + GFF5511 0.13 -1.4 141,970 + GFF5511 0.13 -0.7 141,971 - GFF5511 0.13 -0.9 141,971 - GFF5511 0.13 +0.9 141,971 - GFF5511 0.13 +0.2 141,971 - GFF5511 0.13 -0.3 141,971 - GFF5511 0.13 -0.7 141,971 - GFF5511 0.13 +0.2 141,971 - GFF5511 0.13 +0.3 141,971 - GFF5511 0.13 -0.1 141,971 - GFF5511 0.13 +0.3 141,971 - GFF5511 0.13 -0.0 141,971 - GFF5511 0.13 -0.1 141,971 - GFF5511 0.13 +1.2 141,971 - GFF5511 0.13 +0.2 141,971 - GFF5511 0.13 +1.0 141,971 - GFF5511 0.13 +0.5 141,971 - GFF5511 0.13 -0.3 141,971 - GFF5511 0.13 -0.6 141,971 - GFF5511 0.13 +1.2 141,971 - GFF5511 0.13 -0.3 141,971 - GFF5511 0.13 +0.1 142,297 + GFF5511 0.49 -0.7 142,297 + GFF5511 0.49 +0.7 142,297 + GFF5511 0.49 -0.2 142,297 + GFF5511 0.49 +0.2 142,297 + GFF5511 0.49 +0.1 142,297 + GFF5511 0.49 +0.5 142,297 + GFF5511 0.49 +0.2 142,297 + GFF5511 0.49 -0.5 142,297 + GFF5511 0.49 +0.2 142,297 + GFF5511 0.49 +0.4 142,297 + GFF5511 0.49 +1.1 142,297 + GFF5511 0.49 +0.7 142,297 + GFF5511 0.49 +0.2 142,298 - GFF5511 0.49 -1.7 142,298 - GFF5511 0.49 +0.2 142,298 - GFF5511 0.49 -0.2 142,298 - GFF5511 0.49 +0.0 142,298 - GFF5511 0.49 +0.2 142,298 - GFF5511 0.49 +0.4 142,298 - GFF5511 0.49 +0.2 142,298 - GFF5511 0.49 -1.5 142,298 - GFF5511 0.49 -0.8 142,298 - GFF5511 0.49 +0.0 142,298 - GFF5511 0.49 +1.8 142,627 + GFF5511 0.84 +0.8 142,627 + GFF5511 0.84 -1.0 142,628 - GFF5511 0.85 -0.2 142,628 - GFF5511 0.85 -0.8 142,793 + +0.9 142,793 + -0.2 142,794 - +0.1 142,831 + -0.2 142,831 + +0.8 142,831 + -0.2 142,831 + +0.2 142,831 + -1.2 142,832 - +1.8 142,832 - +0.2 142,832 - +0.8 142,832 - +0.2 142,832 - -1.4 142,832 - +0.8 142,832 - -0.2 142,956 + GFF5512 0.27 +0.2 142,957 - GFF5512 0.27 -2.0 143,109 + GFF5512 0.55 -0.8 143,109 + GFF5512 0.55 -2.2 143,109 + GFF5512 0.55 -0.8 143,109 + GFF5512 0.55 -1.4 143,109 + GFF5512 0.55 -2.3 143,109 + GFF5512 0.55 -2.6 143,109 + GFF5512 0.55 -2.0 143,109 + GFF5512 0.55 -0.5 143,110 - GFF5512 0.55 +0.2 143,110 - GFF5512 0.55 +0.2 143,459 + -1.5 143,459 + -0.5 143,534 + GFF5513 0.15 -1.5 143,534 + GFF5513 0.15 +0.2
Or see this region's nucleotide sequence