Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5458 and GFF5459 are separated by 5 nucleotides GFF5459 and GFF5460 overlap by 4 nucleotides GFF5460 and GFF5461 are separated by 12 nucleotides
GFF5458 - D-erythronate dehydrogenase (NAD+), at 88,797 to 89,813
GFF5458
GFF5459 - 3-oxo-tetronate kinase, at 89,819 to 91,090
GFF5459
GFF5460 - 3-oxo-tetronate 4-phosphate decarboxylase, at 91,087 to 91,752
GFF5460
GFF5461 - 2-dehydrotetronate isomerase (EC 5.3.1.35), at 91,765 to 92,583
GFF5461
Position (kb) 89
90
91
92 Strain fitness (log2 ratio) -2
-1
0
1 at 89.263 kb on - strand, within GFF5458 at 89.263 kb on - strand, within GFF5458 at 89.263 kb on - strand, within GFF5458 at 89.764 kb on - strand at 89.764 kb on - strand at 89.764 kb on - strand at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.063 kb on - strand, within GFF5459 at 90.063 kb on - strand, within GFF5459 at 90.063 kb on - strand, within GFF5459 at 90.071 kb on + strand, within GFF5459 at 90.071 kb on + strand, within GFF5459 at 90.072 kb on - strand, within GFF5459 at 90.163 kb on + strand, within GFF5459 at 90.164 kb on - strand, within GFF5459 at 90.164 kb on - strand, within GFF5459 at 90.206 kb on + strand, within GFF5459 at 90.206 kb on + strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.336 kb on - strand, within GFF5459 at 90.753 kb on - strand, within GFF5459 at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.076 kb on - strand at 91.076 kb on - strand at 91.076 kb on - strand at 91.076 kb on - strand at 91.150 kb on + strand at 91.151 kb on - strand at 91.313 kb on + strand, within GFF5460 at 91.327 kb on + strand, within GFF5460 at 91.328 kb on - strand, within GFF5460 at 91.447 kb on + strand, within GFF5460 at 91.447 kb on + strand, within GFF5460 at 91.447 kb on + strand, within GFF5460 at 91.448 kb on - strand, within GFF5460 at 91.753 kb on + strand at 91.753 kb on + strand at 91.754 kb on - strand at 91.754 kb on - strand at 91.864 kb on + strand, within GFF5461 at 91.864 kb on + strand, within GFF5461 at 91.864 kb on + strand, within GFF5461 at 91.864 kb on + strand, within GFF5461 at 91.865 kb on - strand, within GFF5461 at 91.865 kb on - strand, within GFF5461 at 91.865 kb on - strand, within GFF5461 at 91.876 kb on + strand, within GFF5461
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 89,263 - GFF5458 0.46 -0.6 89,263 - GFF5458 0.46 +0.8 89,263 - GFF5458 0.46 +1.0 89,764 - +1.2 89,764 - +0.0 89,764 - -0.6 90,062 + GFF5459 0.19 +0.0 90,062 + GFF5459 0.19 -0.0 90,062 + GFF5459 0.19 +0.3 90,062 + GFF5459 0.19 -0.8 90,062 + GFF5459 0.19 -0.5 90,062 + GFF5459 0.19 +0.6 90,062 + GFF5459 0.19 -0.2 90,062 + GFF5459 0.19 -0.7 90,062 + GFF5459 0.19 +1.4 90,063 - GFF5459 0.19 +0.4 90,063 - GFF5459 0.19 +0.1 90,063 - GFF5459 0.19 +0.1 90,071 + GFF5459 0.20 -1.1 90,071 + GFF5459 0.20 +0.2 90,072 - GFF5459 0.20 -0.5 90,163 + GFF5459 0.27 -1.4 90,164 - GFF5459 0.27 +0.8 90,164 - GFF5459 0.27 +0.5 90,206 + GFF5459 0.30 -1.6 90,206 + GFF5459 0.30 -0.5 90,207 - GFF5459 0.31 +1.1 90,207 - GFF5459 0.31 +0.2 90,207 - GFF5459 0.31 +1.3 90,207 - GFF5459 0.31 -0.3 90,336 - GFF5459 0.41 -1.0 90,753 - GFF5459 0.73 -0.2 91,075 + +0.4 91,075 + -1.8 91,075 + -0.7 91,075 + -1.2 91,075 + -1.1 91,075 + -0.3 91,075 + +1.2 91,075 + -0.6 91,075 + -0.3 91,075 + -0.5 91,076 - -0.4 91,076 - -1.5 91,076 - +0.5 91,076 - -0.3 91,150 + -0.4 91,151 - -0.3 91,313 + GFF5460 0.34 -1.1 91,327 + GFF5460 0.36 +0.2 91,328 - GFF5460 0.36 +1.0 91,447 + GFF5460 0.54 +0.1 91,447 + GFF5460 0.54 +0.2 91,447 + GFF5460 0.54 -0.3 91,448 - GFF5460 0.54 -1.0 91,753 + -0.8 91,753 + -0.7 91,754 - -1.1 91,754 - -0.5 91,864 + GFF5461 0.12 +0.4 91,864 + GFF5461 0.12 +0.1 91,864 + GFF5461 0.12 +0.0 91,864 + GFF5461 0.12 +0.6 91,865 - GFF5461 0.12 -0.1 91,865 - GFF5461 0.12 -0.8 91,865 - GFF5461 0.12 -0.6 91,876 + GFF5461 0.14 -0.9
Or see this region's nucleotide sequence