Strain Fitness in Variovorax sp. SCN45 around GFF5341

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5339 and GFF5340 are separated by 33 nucleotidesGFF5340 and GFF5341 overlap by 4 nucleotidesGFF5341 and GFF5342 are separated by 20 nucleotidesGFF5342 and GFF5343 are separated by 41 nucleotides GFF5339 - Phospholipid ABC transporter-binding protein MlaB, at 219,147 to 219,422 GFF5339 GFF5340 - Efflux ABC transporter, ATP-binding protein, at 219,456 to 220,403 GFF5340 GFF5341 - Efflux ABC transporter, permease protein, at 220,400 to 221,164 GFF5341 GFF5342 - YrbA protein, at 221,185 to 221,427 GFF5342 GFF5343 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 221,469 to 222,761 GFF5343 Position (kb) 220 221 222Strain fitness (log2 ratio) -2 -1 0 1 2 3at 219.428 kb on + strandat 219.492 kb on + strandat 219.492 kb on + strandat 219.492 kb on + strandat 219.492 kb on + strandat 219.492 kb on + strandat 219.492 kb on + strandat 219.492 kb on + strandat 219.492 kb on + strandat 219.493 kb on - strandat 219.493 kb on - strandat 219.493 kb on - strandat 219.493 kb on - strandat 219.493 kb on - strandat 219.493 kb on - strandat 219.699 kb on + strand, within GFF5340at 219.699 kb on + strand, within GFF5340at 219.795 kb on + strand, within GFF5340at 219.796 kb on - strand, within GFF5340at 219.796 kb on - strand, within GFF5340at 220.037 kb on + strand, within GFF5340at 220.038 kb on - strand, within GFF5340at 220.038 kb on - strand, within GFF5340at 220.038 kb on - strand, within GFF5340at 220.038 kb on - strand, within GFF5340at 220.059 kb on + strand, within GFF5340at 220.059 kb on + strand, within GFF5340at 220.059 kb on + strand, within GFF5340at 220.059 kb on + strand, within GFF5340at 220.060 kb on - strand, within GFF5340at 220.060 kb on - strand, within GFF5340at 220.433 kb on + strandat 220.433 kb on + strandat 220.433 kb on + strandat 220.433 kb on + strandat 220.433 kb on + strandat 220.433 kb on + strandat 220.433 kb on + strandat 220.433 kb on + strandat 220.434 kb on - strandat 220.505 kb on + strand, within GFF5341at 220.505 kb on + strand, within GFF5341at 220.505 kb on + strand, within GFF5341at 220.505 kb on + strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.506 kb on - strand, within GFF5341at 220.550 kb on + strand, within GFF5341at 220.550 kb on + strand, within GFF5341at 220.551 kb on - strand, within GFF5341at 220.565 kb on + strand, within GFF5341at 220.718 kb on + strand, within GFF5341at 220.718 kb on + strand, within GFF5341at 220.718 kb on + strand, within GFF5341at 220.719 kb on - strand, within GFF5341at 220.719 kb on - strand, within GFF5341at 220.719 kb on - strand, within GFF5341at 220.719 kb on - strand, within GFF5341at 220.719 kb on - strand, within GFF5341at 220.719 kb on - strand, within GFF5341at 220.958 kb on + strand, within GFF5341at 220.958 kb on + strand, within GFF5341at 220.958 kb on + strand, within GFF5341at 220.958 kb on + strand, within GFF5341at 220.959 kb on - strand, within GFF5341at 220.959 kb on - strand, within GFF5341at 220.959 kb on - strand, within GFF5341at 220.959 kb on - strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.018 kb on + strand, within GFF5341at 221.019 kb on - strand, within GFF5341at 221.019 kb on - strand, within GFF5341at 221.019 kb on - strand, within GFF5341at 221.019 kb on - strand, within GFF5341at 221.019 kb on - strand, within GFF5341at 221.040 kb on - strand, within GFF5341at 221.040 kb on - strand, within GFF5341at 221.269 kb on + strand, within GFF5342at 221.269 kb on + strand, within GFF5342at 221.269 kb on + strand, within GFF5342at 221.270 kb on - strand, within GFF5342at 221.332 kb on + strand, within GFF5342at 221.332 kb on + strand, within GFF5342at 221.332 kb on + strand, within GFF5342at 221.333 kb on - strand, within GFF5342at 221.333 kb on - strand, within GFF5342at 221.333 kb on - strand, within GFF5342at 221.333 kb on - strand, within GFF5342at 221.358 kb on + strand, within GFF5342at 221.359 kb on - strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.392 kb on + strand, within GFF5342at 221.393 kb on - strand, within GFF5342at 221.393 kb on - strand, within GFF5342at 221.426 kb on - strandat 221.516 kb on - strandat 221.516 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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219,428 + -0.5
219,492 + +0.0
219,492 + -0.2
219,492 + +0.4
219,492 + +0.2
219,492 + -0.3
219,492 + -1.0
219,492 + +1.9
219,492 + -0.0
219,493 - +0.1
219,493 - -0.2
219,493 - -0.5
219,493 - -0.4
219,493 - +0.5
219,493 - -0.1
219,699 + GFF5340 0.26 +0.3
219,699 + GFF5340 0.26 -0.2
219,795 + GFF5340 0.36 +0.0
219,796 - GFF5340 0.36 +0.4
219,796 - GFF5340 0.36 +0.4
220,037 + GFF5340 0.61 -0.2
220,038 - GFF5340 0.61 +0.1
220,038 - GFF5340 0.61 -0.3
220,038 - GFF5340 0.61 -1.4
220,038 - GFF5340 0.61 +3.2
220,059 + GFF5340 0.64 +0.0
220,059 + GFF5340 0.64 +1.2
220,059 + GFF5340 0.64 +0.1
220,059 + GFF5340 0.64 -2.0
220,060 - GFF5340 0.64 +0.5
220,060 - GFF5340 0.64 +0.2
220,433 + -0.8
220,433 + -0.1
220,433 + +0.6
220,433 + +0.2
220,433 + -0.8
220,433 + -0.2
220,433 + +0.4
220,433 + +0.0
220,434 - +0.3
220,505 + GFF5341 0.14 +0.3
220,505 + GFF5341 0.14 -0.5
220,505 + GFF5341 0.14 +0.4
220,505 + GFF5341 0.14 -0.3
220,506 - GFF5341 0.14 +0.2
220,506 - GFF5341 0.14 +0.6
220,506 - GFF5341 0.14 +0.1
220,506 - GFF5341 0.14 -0.8
220,506 - GFF5341 0.14 +0.2
220,506 - GFF5341 0.14 -1.4
220,506 - GFF5341 0.14 -1.2
220,506 - GFF5341 0.14 +0.2
220,550 + GFF5341 0.20 -0.5
220,550 + GFF5341 0.20 +0.1
220,551 - GFF5341 0.20 +0.2
220,565 + GFF5341 0.22 +0.2
220,718 + GFF5341 0.42 -0.4
220,718 + GFF5341 0.42 -0.2
220,718 + GFF5341 0.42 -0.4
220,719 - GFF5341 0.42 +0.8
220,719 - GFF5341 0.42 +0.3
220,719 - GFF5341 0.42 -0.5
220,719 - GFF5341 0.42 -1.0
220,719 - GFF5341 0.42 -0.1
220,719 - GFF5341 0.42 +0.9
220,958 + GFF5341 0.73 -0.6
220,958 + GFF5341 0.73 -0.3
220,958 + GFF5341 0.73 -0.3
220,958 + GFF5341 0.73 +0.1
220,959 - GFF5341 0.73 +0.4
220,959 - GFF5341 0.73 +0.3
220,959 - GFF5341 0.73 -0.1
220,959 - GFF5341 0.73 -0.6
221,018 + GFF5341 0.81 +0.1
221,018 + GFF5341 0.81 -0.2
221,018 + GFF5341 0.81 -0.4
221,018 + GFF5341 0.81 +0.2
221,018 + GFF5341 0.81 +0.0
221,018 + GFF5341 0.81 -0.1
221,018 + GFF5341 0.81 -0.3
221,018 + GFF5341 0.81 +1.7
221,018 + GFF5341 0.81 -0.1
221,018 + GFF5341 0.81 +0.9
221,018 + GFF5341 0.81 -0.2
221,019 - GFF5341 0.81 -0.3
221,019 - GFF5341 0.81 +0.6
221,019 - GFF5341 0.81 +0.6
221,019 - GFF5341 0.81 +0.9
221,019 - GFF5341 0.81 +0.1
221,040 - GFF5341 0.84 -1.4
221,040 - GFF5341 0.84 +0.1
221,269 + GFF5342 0.35 +0.0
221,269 + GFF5342 0.35 +0.1
221,269 + GFF5342 0.35 -1.1
221,270 - GFF5342 0.35 -0.7
221,332 + GFF5342 0.60 -0.9
221,332 + GFF5342 0.60 +0.1
221,332 + GFF5342 0.60 +0.4
221,333 - GFF5342 0.61 -0.3
221,333 - GFF5342 0.61 +0.2
221,333 - GFF5342 0.61 -0.6
221,333 - GFF5342 0.61 +0.4
221,358 + GFF5342 0.71 -0.6
221,359 - GFF5342 0.72 +0.0
221,392 + GFF5342 0.85 -0.4
221,392 + GFF5342 0.85 -0.1
221,392 + GFF5342 0.85 +0.5
221,392 + GFF5342 0.85 -1.9
221,392 + GFF5342 0.85 +0.8
221,392 + GFF5342 0.85 +0.3
221,392 + GFF5342 0.85 +0.7
221,392 + GFF5342 0.85 +0.4
221,392 + GFF5342 0.85 -1.2
221,392 + GFF5342 0.85 -1.2
221,392 + GFF5342 0.85 -0.2
221,392 + GFF5342 0.85 +0.2
221,392 + GFF5342 0.85 -0.4
221,392 + GFF5342 0.85 +0.3
221,392 + GFF5342 0.85 -0.3
221,392 + GFF5342 0.85 -0.9
221,393 - GFF5342 0.86 +0.1
221,393 - GFF5342 0.86 +0.1
221,426 - -0.7
221,516 - -0.5
221,516 - +0.6

Or see this region's nucleotide sequence