Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5320 and GFF5321 are separated by 6 nucleotides GFF5321 and GFF5322 overlap by 4 nucleotides GFF5322 and GFF5323 overlap by 4 nucleotides
GFF5320 - Type IV pilus biogenesis protein PilN, at 197,820 to 198,443
GFF5320
GFF5321 - Type IV pilus biogenesis protein PilO, at 198,450 to 199,127
GFF5321
GFF5322 - Type IV pilus biogenesis protein PilP, at 199,124 to 199,663
GFF5322
GFF5323 - Type IV pilus biogenesis protein PilQ, at 199,660 to 201,840
GFF5323
Position (kb)
199
200 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 198.159 kb on + strand, within GFF5320 at 198.159 kb on + strand, within GFF5320 at 198.159 kb on + strand, within GFF5320 at 198.159 kb on + strand, within GFF5320 at 198.159 kb on + strand, within GFF5320 at 198.159 kb on + strand, within GFF5320 at 198.160 kb on - strand, within GFF5320 at 198.160 kb on - strand, within GFF5320 at 198.245 kb on + strand, within GFF5320 at 198.245 kb on + strand, within GFF5320 at 198.469 kb on + strand at 198.633 kb on + strand, within GFF5321 at 198.687 kb on + strand, within GFF5321 at 198.687 kb on + strand, within GFF5321 at 198.688 kb on - strand, within GFF5321 at 198.688 kb on - strand, within GFF5321 at 198.688 kb on - strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.891 kb on + strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.934 kb on - strand, within GFF5321 at 198.934 kb on - strand, within GFF5321 at 198.934 kb on - strand, within GFF5321 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.508 kb on + strand, within GFF5322 at 199.508 kb on + strand, within GFF5322 at 199.508 kb on + strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.511 kb on + strand, within GFF5322 at 199.529 kb on + strand, within GFF5322 at 199.529 kb on + strand, within GFF5322 at 199.530 kb on - strand, within GFF5322 at 199.530 kb on - strand, within GFF5322 at 199.530 kb on - strand, within GFF5322 at 199.544 kb on + strand, within GFF5322 at 199.544 kb on + strand, within GFF5322 at 199.544 kb on + strand, within GFF5322 at 199.545 kb on - strand, within GFF5322 at 199.629 kb on + strand at 199.629 kb on + strand at 199.629 kb on + strand at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.039 kb on + strand, within GFF5323 at 200.166 kb on + strand, within GFF5323 at 200.166 kb on + strand, within GFF5323 at 200.166 kb on + strand, within GFF5323 at 200.167 kb on - strand, within GFF5323 at 200.167 kb on - strand, within GFF5323 at 200.167 kb on - strand, within GFF5323 at 200.395 kb on + strand, within GFF5323 at 200.395 kb on + strand, within GFF5323 at 200.396 kb on - strand, within GFF5323 at 200.473 kb on + strand, within GFF5323 at 200.474 kb on - strand, within GFF5323 at 200.513 kb on - strand, within GFF5323
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 198,159 + GFF5320 0.54 -2.4 198,159 + GFF5320 0.54 +0.2 198,159 + GFF5320 0.54 -1.2 198,159 + GFF5320 0.54 -0.5 198,159 + GFF5320 0.54 -1.9 198,159 + GFF5320 0.54 -1.8 198,160 - GFF5320 0.54 -1.0 198,160 - GFF5320 0.54 -1.2 198,245 + GFF5320 0.68 -1.9 198,245 + GFF5320 0.68 -1.6 198,469 + -0.1 198,633 + GFF5321 0.27 +0.0 198,687 + GFF5321 0.35 -1.9 198,687 + GFF5321 0.35 -1.7 198,688 - GFF5321 0.35 -0.7 198,688 - GFF5321 0.35 -1.6 198,688 - GFF5321 0.35 +0.1 198,756 + GFF5321 0.45 -1.0 198,756 + GFF5321 0.45 -0.8 198,756 + GFF5321 0.45 -1.2 198,756 + GFF5321 0.45 -1.3 198,756 + GFF5321 0.45 -1.5 198,756 + GFF5321 0.45 -0.1 198,757 - GFF5321 0.45 -0.7 198,757 - GFF5321 0.45 -1.3 198,757 - GFF5321 0.45 -1.2 198,757 - GFF5321 0.45 -1.5 198,757 - GFF5321 0.45 -0.9 198,757 - GFF5321 0.45 -1.5 198,757 - GFF5321 0.45 -0.5 198,757 - GFF5321 0.45 -1.5 198,757 - GFF5321 0.45 -1.9 198,891 + GFF5321 0.65 -0.9 198,892 - GFF5321 0.65 -3.6 198,892 - GFF5321 0.65 -0.9 198,892 - GFF5321 0.65 -1.2 198,892 - GFF5321 0.65 -1.2 198,892 - GFF5321 0.65 -0.7 198,892 - GFF5321 0.65 -0.7 198,892 - GFF5321 0.65 -1.2 198,892 - GFF5321 0.65 -1.7 198,892 - GFF5321 0.65 -1.2 198,894 + GFF5321 0.65 -0.4 198,894 + GFF5321 0.65 -1.2 198,894 + GFF5321 0.65 -1.2 198,894 + GFF5321 0.65 -0.4 198,894 + GFF5321 0.65 -1.4 198,894 + GFF5321 0.65 -0.8 198,894 + GFF5321 0.65 -0.2 198,894 + GFF5321 0.65 -1.0 198,894 + GFF5321 0.65 -1.8 198,895 - GFF5321 0.66 -1.2 198,895 - GFF5321 0.66 -1.4 198,895 - GFF5321 0.66 -1.2 198,895 - GFF5321 0.66 -2.0 198,895 - GFF5321 0.66 -1.1 198,895 - GFF5321 0.66 -1.0 198,895 - GFF5321 0.66 -0.8 198,895 - GFF5321 0.66 -1.0 198,895 - GFF5321 0.66 -1.7 198,933 + GFF5321 0.71 -1.4 198,933 + GFF5321 0.71 -0.3 198,933 + GFF5321 0.71 -1.7 198,933 + GFF5321 0.71 -0.2 198,933 + GFF5321 0.71 -1.2 198,934 - GFF5321 0.71 -1.5 198,934 - GFF5321 0.71 -1.3 198,934 - GFF5321 0.71 -1.8 199,289 + GFF5322 0.31 -1.8 199,289 + GFF5322 0.31 -1.4 199,289 + GFF5322 0.31 -1.7 199,289 + GFF5322 0.31 -0.4 199,289 + GFF5322 0.31 -1.2 199,289 + GFF5322 0.31 -2.0 199,289 + GFF5322 0.31 -1.4 199,289 + GFF5322 0.31 -1.2 199,289 + GFF5322 0.31 -0.1 199,290 - GFF5322 0.31 -2.0 199,290 - GFF5322 0.31 -1.4 199,290 - GFF5322 0.31 -0.4 199,290 - GFF5322 0.31 -1.4 199,290 - GFF5322 0.31 -1.1 199,508 + GFF5322 0.71 +0.0 199,508 + GFF5322 0.71 -1.8 199,508 + GFF5322 0.71 -2.4 199,509 - GFF5322 0.71 -2.6 199,509 - GFF5322 0.71 -2.7 199,509 - GFF5322 0.71 -0.2 199,509 - GFF5322 0.71 -2.5 199,509 - GFF5322 0.71 -1.2 199,509 - GFF5322 0.71 -0.8 199,509 - GFF5322 0.71 -1.2 199,511 + GFF5322 0.72 -1.2 199,529 + GFF5322 0.75 -1.4 199,529 + GFF5322 0.75 -1.0 199,530 - GFF5322 0.75 +0.2 199,530 - GFF5322 0.75 -0.3 199,530 - GFF5322 0.75 -1.9 199,544 + GFF5322 0.78 -2.6 199,544 + GFF5322 0.78 -1.9 199,544 + GFF5322 0.78 -2.4 199,545 - GFF5322 0.78 -0.9 199,629 + -0.8 199,629 + -1.5 199,629 + -0.5 200,011 + GFF5323 0.16 +0.5 200,011 + GFF5323 0.16 +0.1 200,011 + GFF5323 0.16 -0.7 200,011 + GFF5323 0.16 -0.8 200,011 + GFF5323 0.16 -1.4 200,011 + GFF5323 0.16 -1.5 200,011 + GFF5323 0.16 -1.8 200,011 + GFF5323 0.16 -1.7 200,012 - GFF5323 0.16 -1.1 200,012 - GFF5323 0.16 -1.3 200,012 - GFF5323 0.16 -0.4 200,012 - GFF5323 0.16 -1.3 200,012 - GFF5323 0.16 -1.6 200,012 - GFF5323 0.16 +0.0 200,012 - GFF5323 0.16 -1.0 200,012 - GFF5323 0.16 -0.7 200,012 - GFF5323 0.16 -2.4 200,012 - GFF5323 0.16 -1.0 200,039 + GFF5323 0.17 -1.4 200,166 + GFF5323 0.23 -0.2 200,166 + GFF5323 0.23 -0.4 200,166 + GFF5323 0.23 -1.6 200,167 - GFF5323 0.23 -1.7 200,167 - GFF5323 0.23 -0.5 200,167 - GFF5323 0.23 -1.6 200,395 + GFF5323 0.34 -0.1 200,395 + GFF5323 0.34 -1.2 200,396 - GFF5323 0.34 -1.4 200,473 + GFF5323 0.37 -0.7 200,474 - GFF5323 0.37 -1.2 200,513 - GFF5323 0.39 -1.4
Or see this region's nucleotide sequence