Strain Fitness in Variovorax sp. SCN45 around GFF5312

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5311 and GFF5312 overlap by 4 nucleotidesGFF5312 and GFF5313 are separated by 116 nucleotides GFF5311 - Cytochrome c-type biogenesis protein Ccs1/ResB, at 186,892 to 189,012 GFF5311 GFF5312 - Cytochrome c-type biogenesis protein CcsA/ResC, at 189,009 to 190,325 GFF5312 GFF5313 - Protein-methionine-sulfoxide reductase catalytic subunit MsrP, at 190,442 to 191,467 GFF5313 Position (kb) 189 190 191Strain fitness (log2 ratio) -2 -1 0 1 2at 190.311 kb on + strandat 190.311 kb on + strandat 190.312 kb on - strandat 190.312 kb on - strandat 190.323 kb on + strandat 190.323 kb on + strandat 190.323 kb on + strandat 190.323 kb on + strandat 190.323 kb on + strandat 190.323 kb on + strandat 190.324 kb on - strandat 190.324 kb on - strandat 190.324 kb on - strandat 190.406 kb on + strandat 190.406 kb on + strandat 190.406 kb on + strandat 190.406 kb on + strandat 190.407 kb on - strandat 190.407 kb on - strandat 190.560 kb on + strand, within GFF5313at 190.560 kb on + strand, within GFF5313at 190.560 kb on + strand, within GFF5313at 190.560 kb on + strand, within GFF5313at 190.561 kb on - strand, within GFF5313at 190.561 kb on - strand, within GFF5313at 190.715 kb on + strand, within GFF5313at 190.715 kb on + strand, within GFF5313at 190.715 kb on + strand, within GFF5313at 190.715 kb on + strand, within GFF5313at 190.715 kb on + strand, within GFF5313at 190.716 kb on - strand, within GFF5313at 190.716 kb on - strand, within GFF5313at 190.716 kb on - strand, within GFF5313at 190.716 kb on - strand, within GFF5313at 190.733 kb on + strand, within GFF5313at 190.733 kb on + strand, within GFF5313at 190.733 kb on + strand, within GFF5313at 190.733 kb on + strand, within GFF5313at 190.733 kb on + strand, within GFF5313at 190.733 kb on + strand, within GFF5313at 190.734 kb on - strand, within GFF5313at 190.734 kb on - strand, within GFF5313at 190.734 kb on - strand, within GFF5313at 190.734 kb on - strand, within GFF5313at 190.734 kb on - strand, within GFF5313at 190.745 kb on + strand, within GFF5313at 190.745 kb on + strand, within GFF5313at 190.745 kb on + strand, within GFF5313at 190.746 kb on - strand, within GFF5313at 190.746 kb on - strand, within GFF5313at 190.899 kb on - strand, within GFF5313at 190.899 kb on - strand, within GFF5313at 190.899 kb on - strand, within GFF5313at 190.899 kb on - strand, within GFF5313at 190.899 kb on - strand, within GFF5313at 190.949 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.072 kb on + strand, within GFF5313at 191.073 kb on - strand, within GFF5313at 191.073 kb on - strand, within GFF5313at 191.073 kb on - strand, within GFF5313at 191.073 kb on - strand, within GFF5313at 191.073 kb on - strand, within GFF5313at 191.073 kb on - strand, within GFF5313at 191.135 kb on + strand, within GFF5313at 191.135 kb on + strand, within GFF5313at 191.135 kb on + strand, within GFF5313at 191.135 kb on + strand, within GFF5313at 191.136 kb on - strand, within GFF5313at 191.136 kb on - strand, within GFF5313at 191.136 kb on - strand, within GFF5313at 191.136 kb on - strand, within GFF5313at 191.136 kb on - strand, within GFF5313at 191.136 kb on - strand, within GFF5313at 191.195 kb on + strand, within GFF5313at 191.195 kb on + strand, within GFF5313at 191.195 kb on + strand, within GFF5313at 191.196 kb on - strand, within GFF5313at 191.196 kb on - strand, within GFF5313at 191.285 kb on + strand, within GFF5313at 191.285 kb on + strand, within GFF5313at 191.286 kb on - strand, within GFF5313at 191.286 kb on - strand, within GFF5313at 191.294 kb on + strand, within GFF5313at 191.295 kb on - strand, within GFF5313at 191.295 kb on - strand, within GFF5313

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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190,311 + -1.6
190,311 + +2.6
190,312 - -1.0
190,312 - +0.4
190,323 + -0.1
190,323 + -0.3
190,323 + -0.3
190,323 + +2.2
190,323 + -0.4
190,323 + -0.2
190,324 - +0.7
190,324 - +0.3
190,324 - -1.1
190,406 + +0.9
190,406 + -0.1
190,406 + +0.4
190,406 + +0.0
190,407 - -0.4
190,407 - -0.1
190,560 + GFF5313 0.12 +1.6
190,560 + GFF5313 0.12 -0.6
190,560 + GFF5313 0.12 +1.1
190,560 + GFF5313 0.12 -0.6
190,561 - GFF5313 0.12 -0.1
190,561 - GFF5313 0.12 +0.6
190,715 + GFF5313 0.27 +0.6
190,715 + GFF5313 0.27 -1.2
190,715 + GFF5313 0.27 -0.2
190,715 + GFF5313 0.27 +1.0
190,715 + GFF5313 0.27 -0.7
190,716 - GFF5313 0.27 +0.0
190,716 - GFF5313 0.27 -0.3
190,716 - GFF5313 0.27 -0.4
190,716 - GFF5313 0.27 -0.4
190,733 + GFF5313 0.28 +1.0
190,733 + GFF5313 0.28 -1.1
190,733 + GFF5313 0.28 -0.4
190,733 + GFF5313 0.28 -0.4
190,733 + GFF5313 0.28 -0.2
190,733 + GFF5313 0.28 +1.9
190,734 - GFF5313 0.28 -0.2
190,734 - GFF5313 0.28 -0.8
190,734 - GFF5313 0.28 -0.4
190,734 - GFF5313 0.28 -0.2
190,734 - GFF5313 0.28 +0.0
190,745 + GFF5313 0.30 -1.4
190,745 + GFF5313 0.30 -0.1
190,745 + GFF5313 0.30 +1.3
190,746 - GFF5313 0.30 -1.7
190,746 - GFF5313 0.30 +1.1
190,899 - GFF5313 0.45 -1.1
190,899 - GFF5313 0.45 +0.6
190,899 - GFF5313 0.45 -0.1
190,899 - GFF5313 0.45 +0.7
190,899 - GFF5313 0.45 +0.4
190,949 + GFF5313 0.49 -0.2
191,072 + GFF5313 0.61 -0.1
191,072 + GFF5313 0.61 -0.1
191,072 + GFF5313 0.61 +0.1
191,072 + GFF5313 0.61 +0.9
191,072 + GFF5313 0.61 -0.5
191,072 + GFF5313 0.61 -0.9
191,072 + GFF5313 0.61 -1.1
191,072 + GFF5313 0.61 -0.1
191,072 + GFF5313 0.61 +0.9
191,073 - GFF5313 0.62 -0.2
191,073 - GFF5313 0.62 -1.5
191,073 - GFF5313 0.62 +0.4
191,073 - GFF5313 0.62 -0.7
191,073 - GFF5313 0.62 -0.7
191,073 - GFF5313 0.62 +0.9
191,135 + GFF5313 0.68 -0.5
191,135 + GFF5313 0.68 -0.5
191,135 + GFF5313 0.68 -0.4
191,135 + GFF5313 0.68 +0.2
191,136 - GFF5313 0.68 +0.1
191,136 - GFF5313 0.68 +0.3
191,136 - GFF5313 0.68 -2.4
191,136 - GFF5313 0.68 -0.6
191,136 - GFF5313 0.68 +0.6
191,136 - GFF5313 0.68 +0.6
191,195 + GFF5313 0.73 +1.2
191,195 + GFF5313 0.73 +0.2
191,195 + GFF5313 0.73 -0.5
191,196 - GFF5313 0.73 -0.3
191,196 - GFF5313 0.73 -0.8
191,285 + GFF5313 0.82 +0.0
191,285 + GFF5313 0.82 -0.2
191,286 - GFF5313 0.82 +0.1
191,286 - GFF5313 0.82 -1.7
191,294 + GFF5313 0.83 -0.1
191,295 - GFF5313 0.83 -0.1
191,295 - GFF5313 0.83 -0.8

Or see this region's nucleotide sequence