Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5110 and GFF5111 are separated by 64 nucleotides GFF5111 and GFF5112 are separated by 9 nucleotides GFF5112 and GFF5113 are separated by 15 nucleotides GFF5113 and GFF5114 are separated by 89 nucleotides
GFF5110 - Putative transmembrane protein, at 211,559 to 212,863
GFF5110
GFF5111 - Permease of the drug/metabolite transporter (DMT) superfamily, at 212,928 to 213,818
GFF5111
GFF5112 - Glutathione peroxidase (EC 1.11.1.9) @ Thioredoxin peroxidase (EC 1.11.1.15), at 213,828 to 214,316
GFF5112
GFF5113 - hypothetical protein, at 214,332 to 214,775
GFF5113
GFF5114 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 214,865 to 216,097
GFF5114
Position (kb) 213
214
215 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 213.039 kb on + strand, within GFF5111 at 213.039 kb on + strand, within GFF5111 at 213.039 kb on + strand, within GFF5111 at 213.039 kb on + strand, within GFF5111 at 213.039 kb on + strand, within GFF5111 at 213.039 kb on + strand, within GFF5111 at 213.039 kb on + strand, within GFF5111 at 213.040 kb on - strand, within GFF5111 at 213.040 kb on - strand, within GFF5111 at 213.040 kb on - strand, within GFF5111 at 213.204 kb on + strand, within GFF5111 at 213.204 kb on + strand, within GFF5111 at 213.205 kb on - strand, within GFF5111 at 213.609 kb on + strand, within GFF5111 at 213.610 kb on - strand, within GFF5111 at 213.840 kb on + strand at 213.840 kb on + strand at 213.841 kb on - strand at 213.981 kb on + strand, within GFF5112 at 213.981 kb on + strand, within GFF5112 at 213.981 kb on + strand, within GFF5112 at 213.999 kb on - strand, within GFF5112 at 213.999 kb on - strand, within GFF5112 at 214.083 kb on + strand, within GFF5112 at 214.083 kb on + strand, within GFF5112 at 214.084 kb on - strand, within GFF5112 at 214.084 kb on - strand, within GFF5112 at 214.084 kb on - strand, within GFF5112 at 214.084 kb on - strand, within GFF5112 at 214.146 kb on + strand, within GFF5112 at 214.251 kb on + strand, within GFF5112 at 214.252 kb on - strand, within GFF5112 at 214.333 kb on + strand at 214.333 kb on + strand at 214.333 kb on + strand at 214.333 kb on + strand at 214.333 kb on + strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.424 kb on - strand, within GFF5113 at 214.424 kb on - strand, within GFF5113 at 214.424 kb on - strand, within GFF5113 at 214.534 kb on + strand, within GFF5113 at 214.534 kb on + strand, within GFF5113 at 214.534 kb on + strand, within GFF5113 at 214.534 kb on + strand, within GFF5113 at 214.534 kb on + strand, within GFF5113 at 214.535 kb on - strand, within GFF5113 at 214.535 kb on - strand, within GFF5113 at 214.633 kb on + strand, within GFF5113 at 214.634 kb on - strand, within GFF5113 at 214.722 kb on + strand, within GFF5113 at 214.722 kb on + strand, within GFF5113 at 214.723 kb on - strand, within GFF5113 at 214.723 kb on - strand, within GFF5113 at 215.025 kb on + strand, within GFF5114 at 215.052 kb on + strand, within GFF5114 at 215.052 kb on + strand, within GFF5114 at 215.052 kb on + strand, within GFF5114 at 215.052 kb on + strand, within GFF5114 at 215.053 kb on - strand, within GFF5114 at 215.053 kb on - strand, within GFF5114 at 215.053 kb on - strand, within GFF5114 at 215.053 kb on - strand, within GFF5114 at 215.298 kb on + strand, within GFF5114 at 215.299 kb on - strand, within GFF5114 at 215.299 kb on - strand, within GFF5114 at 215.299 kb on - strand, within GFF5114 at 215.299 kb on - strand, within GFF5114
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 213,039 + GFF5111 0.12 +0.3 213,039 + GFF5111 0.12 -0.8 213,039 + GFF5111 0.12 +0.2 213,039 + GFF5111 0.12 +0.3 213,039 + GFF5111 0.12 -0.7 213,039 + GFF5111 0.12 +0.6 213,039 + GFF5111 0.12 -0.7 213,040 - GFF5111 0.13 -0.3 213,040 - GFF5111 0.13 -0.3 213,040 - GFF5111 0.13 -0.5 213,204 + GFF5111 0.31 -1.0 213,204 + GFF5111 0.31 -0.7 213,205 - GFF5111 0.31 -0.4 213,609 + GFF5111 0.76 -0.1 213,610 - GFF5111 0.77 -0.2 213,840 + -0.4 213,840 + -1.0 213,841 - -0.6 213,981 + GFF5112 0.31 -0.4 213,981 + GFF5112 0.31 +0.2 213,981 + GFF5112 0.31 +0.2 213,999 - GFF5112 0.35 -1.6 213,999 - GFF5112 0.35 -0.0 214,083 + GFF5112 0.52 +0.3 214,083 + GFF5112 0.52 +0.3 214,084 - GFF5112 0.52 +0.2 214,084 - GFF5112 0.52 -0.6 214,084 - GFF5112 0.52 -0.3 214,084 - GFF5112 0.52 +0.4 214,146 + GFF5112 0.65 +0.5 214,251 + GFF5112 0.87 -0.2 214,252 - GFF5112 0.87 -0.2 214,333 + +0.4 214,333 + +1.2 214,333 + +0.1 214,333 + +0.7 214,333 + -0.0 214,334 - -0.4 214,334 - -1.1 214,334 - -2.8 214,334 - +0.6 214,334 - -0.0 214,334 - +0.5 214,334 - +1.2 214,334 - +0.5 214,424 - GFF5113 0.21 -0.4 214,424 - GFF5113 0.21 -0.8 214,424 - GFF5113 0.21 -0.6 214,534 + GFF5113 0.45 +0.2 214,534 + GFF5113 0.45 +0.4 214,534 + GFF5113 0.45 +0.7 214,534 + GFF5113 0.45 +0.4 214,534 + GFF5113 0.45 -1.4 214,535 - GFF5113 0.46 +1.3 214,535 - GFF5113 0.46 +0.2 214,633 + GFF5113 0.68 +0.5 214,634 - GFF5113 0.68 -0.8 214,722 + GFF5113 0.88 +0.3 214,722 + GFF5113 0.88 +1.2 214,723 - GFF5113 0.88 +0.2 214,723 - GFF5113 0.88 +0.4 215,025 + GFF5114 0.13 +1.0 215,052 + GFF5114 0.15 -2.1 215,052 + GFF5114 0.15 +0.1 215,052 + GFF5114 0.15 +0.4 215,052 + GFF5114 0.15 +0.4 215,053 - GFF5114 0.15 +0.6 215,053 - GFF5114 0.15 -1.6 215,053 - GFF5114 0.15 -0.3 215,053 - GFF5114 0.15 -0.9 215,298 + GFF5114 0.35 -1.3 215,299 - GFF5114 0.35 -1.1 215,299 - GFF5114 0.35 +0.5 215,299 - GFF5114 0.35 +0.7 215,299 - GFF5114 0.35 -0.7
Or see this region's nucleotide sequence