Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4732 and GFF4733 are separated by 9 nucleotides GFF4733 and GFF4734 overlap by 4 nucleotides GFF4734 and GFF4735 are separated by 24 nucleotides GFF4735 and GFF4736 overlap by 4 nucleotides
GFF4732 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 97,558 to 97,953
GFF4732
GFF4733 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 97,963 to 98,754
GFF4733
GFF4734 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 98,751 to 101,219
GFF4734
GFF4735 - Nitrate ABC transporter, ATP-binding protein, at 101,244 to 102,053
GFF4735
GFF4736 - Nitrate ABC transporter, permease protein, at 102,050 to 102,970
GFF4736
Position (kb)
98
99
100
101
102 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 97.964 kb on + strand at 97.964 kb on + strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.982 kb on + strand at 97.983 kb on - strand at 98.258 kb on + strand, within GFF4733 at 98.259 kb on - strand, within GFF4733 at 98.345 kb on + strand, within GFF4733 at 98.480 kb on + strand, within GFF4733 at 98.481 kb on - strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.921 kb on - strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.945 kb on - strand at 98.945 kb on - strand at 98.965 kb on + strand at 98.966 kb on - strand at 99.007 kb on + strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.085 kb on - strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.197 kb on - strand, within GFF4734 at 99.311 kb on - strand, within GFF4734 at 99.424 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.599 kb on - strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.682 kb on + strand, within GFF4734 at 99.682 kb on + strand, within GFF4734 at 99.769 kb on + strand, within GFF4734 at 99.770 kb on - strand, within GFF4734 at 100.067 kb on - strand, within GFF4734 at 100.067 kb on - strand, within GFF4734 at 100.141 kb on + strand, within GFF4734 at 100.142 kb on - strand, within GFF4734 at 100.264 kb on + strand, within GFF4734 at 100.264 kb on + strand, within GFF4734 at 100.264 kb on + strand, within GFF4734 at 100.265 kb on - strand, within GFF4734 at 100.378 kb on + strand, within GFF4734 at 100.378 kb on + strand, within GFF4734 at 100.379 kb on - strand, within GFF4734 at 100.901 kb on - strand, within GFF4734 at 101.077 kb on + strand at 101.077 kb on + strand at 101.077 kb on + strand at 101.078 kb on - strand at 101.078 kb on - strand at 101.116 kb on + strand at 101.116 kb on + strand at 101.116 kb on + strand at 101.281 kb on + strand at 101.281 kb on + strand at 101.282 kb on - strand at 101.317 kb on + strand at 101.317 kb on + strand at 101.317 kb on + strand at 101.317 kb on + strand at 101.318 kb on - strand at 101.749 kb on + strand, within GFF4735 at 101.749 kb on + strand, within GFF4735 at 101.749 kb on + strand, within GFF4735 at 101.749 kb on + strand, within GFF4735 at 101.750 kb on - strand, within GFF4735 at 101.750 kb on - strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 102.034 kb on + strand at 102.034 kb on + strand at 102.034 kb on + strand at 102.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 97,964 + -0.3 97,964 + +0.2 97,965 - -0.7 97,965 - +0.3 97,965 - -0.3 97,965 - -0.9 97,982 + +0.5 97,983 - +1.3 98,258 + GFF4733 0.37 -0.5 98,259 - GFF4733 0.37 -0.7 98,345 + GFF4733 0.48 +0.2 98,480 + GFF4733 0.65 -0.3 98,481 - GFF4733 0.65 -1.0 98,609 + GFF4733 0.82 +1.7 98,609 + GFF4733 0.82 -0.7 98,610 - GFF4733 0.82 -0.5 98,610 - GFF4733 0.82 +1.3 98,921 - -1.9 98,944 + -0.7 98,944 + -2.1 98,944 + -0.4 98,945 - -0.3 98,945 - +2.3 98,965 + -0.2 98,966 - +0.3 99,007 + GFF4734 0.10 -1.3 99,008 - GFF4734 0.10 -1.0 99,008 - GFF4734 0.10 -0.9 99,084 + GFF4734 0.13 -0.0 99,084 + GFF4734 0.13 +0.3 99,085 - GFF4734 0.14 -1.3 99,088 + GFF4734 0.14 -0.3 99,088 + GFF4734 0.14 -1.2 99,088 + GFF4734 0.14 +0.3 99,088 + GFF4734 0.14 -0.1 99,088 + GFF4734 0.14 -0.1 99,088 + GFF4734 0.14 -0.5 99,089 - GFF4734 0.14 -1.0 99,089 - GFF4734 0.14 +0.8 99,196 + GFF4734 0.18 +0.1 99,196 + GFF4734 0.18 -0.2 99,197 - GFF4734 0.18 -0.8 99,311 - GFF4734 0.23 +0.2 99,424 + GFF4734 0.27 -0.9 99,502 + GFF4734 0.30 +0.1 99,502 + GFF4734 0.30 -1.3 99,502 + GFF4734 0.30 -0.0 99,502 + GFF4734 0.30 -1.3 99,503 - GFF4734 0.30 -0.3 99,503 - GFF4734 0.30 +1.0 99,598 + GFF4734 0.34 -1.2 99,598 + GFF4734 0.34 -0.0 99,599 - GFF4734 0.34 -0.3 99,601 + GFF4734 0.34 -0.3 99,601 + GFF4734 0.34 +0.4 99,601 + GFF4734 0.34 +0.3 99,601 + GFF4734 0.34 -0.0 99,601 + GFF4734 0.34 -0.9 99,601 + GFF4734 0.34 -1.1 99,601 + GFF4734 0.34 -0.5 99,602 - GFF4734 0.34 -0.9 99,602 - GFF4734 0.34 -0.7 99,682 + GFF4734 0.38 -0.8 99,682 + GFF4734 0.38 +0.1 99,769 + GFF4734 0.41 +1.0 99,770 - GFF4734 0.41 -0.3 100,067 - GFF4734 0.53 -0.3 100,067 - GFF4734 0.53 +0.5 100,141 + GFF4734 0.56 -1.9 100,142 - GFF4734 0.56 -0.0 100,264 + GFF4734 0.61 -1.5 100,264 + GFF4734 0.61 -0.9 100,264 + GFF4734 0.61 +0.5 100,265 - GFF4734 0.61 +0.4 100,378 + GFF4734 0.66 +0.1 100,378 + GFF4734 0.66 +0.7 100,379 - GFF4734 0.66 +1.3 100,901 - GFF4734 0.87 -0.3 101,077 + -0.5 101,077 + -2.1 101,077 + -0.5 101,078 - -1.0 101,078 - +0.1 101,116 + +0.9 101,116 + +1.0 101,116 + -0.6 101,281 + -0.1 101,281 + +1.5 101,282 - -1.3 101,317 + -1.0 101,317 + -2.0 101,317 + -0.9 101,317 + +0.4 101,318 - -0.8 101,749 + GFF4735 0.62 +0.5 101,749 + GFF4735 0.62 -0.4 101,749 + GFF4735 0.62 -1.1 101,749 + GFF4735 0.62 -0.3 101,750 - GFF4735 0.62 -1.9 101,750 - GFF4735 0.62 +0.8 101,751 + GFF4735 0.63 -0.2 101,751 + GFF4735 0.63 -0.7 101,751 + GFF4735 0.63 -0.3 101,751 + GFF4735 0.63 +0.7 101,751 + GFF4735 0.63 +1.6 101,751 + GFF4735 0.63 +1.7 101,751 + GFF4735 0.63 +0.3 101,751 + GFF4735 0.63 +0.0 101,752 - GFF4735 0.63 -0.3 101,752 - GFF4735 0.63 -0.7 101,752 - GFF4735 0.63 -0.5 101,752 - GFF4735 0.63 -0.8 102,034 + -2.0 102,034 + +0.3 102,034 + +0.1 102,035 - -0.1
Or see this region's nucleotide sequence