Strain Fitness in Variovorax sp. SCN45 around GFF4647

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
2,581	+				+0.9
2,581	+				-0.6
2,581	+				-0.1
2,581	+				+0.5
2,581	+				+1.5
2,581	+				-0.6
2,581	+				+0.3
2,581	+				-0.1
2,581	+				-1.1
2,581	+				-1.1
2,582	-				-0.4
2,582	-				+0.6
2,582	-				-0.4
2,582	-				-0.8
2,728	+		GFF4646	0.22	-0.6
2,729	-		GFF4646	0.22	+0.2
2,750	+		GFF4646	0.25	-0.2
2,750	+		GFF4646	0.25	-1.4
2,750	+		GFF4646	0.25	-1.7
2,750	+		GFF4646	0.25	-0.3
2,751	-		GFF4646	0.25	+0.1
2,830	+		GFF4646	0.33	-0.9
2,830	+		GFF4646	0.33	-0.6
2,830	+		GFF4646	0.33	-1.3
2,830	+		GFF4646	0.33	-0.8
2,830	+		GFF4646	0.33	-1.0
2,830	+		GFF4646	0.33	-0.7
2,830	+		GFF4646	0.33	-0.3
2,830	+		GFF4646	0.33	-1.9
2,830	+		GFF4646	0.33	-0.7
2,831	-		GFF4646	0.33	-0.0
2,831	-		GFF4646	0.33	-0.2
2,831	-		GFF4646	0.33	-0.0
2,831	-		GFF4646	0.33	-0.5
2,831	-		GFF4646	0.33	-0.7
2,831	-		GFF4646	0.33	+0.4
2,935	+		GFF4646	0.44	-0.5
2,936	-		GFF4646	0.44	-1.6
2,936	-		GFF4646	0.44	-0.2
2,936	-		GFF4646	0.44	-0.3
2,965	+		GFF4646	0.47	+1.0
3,064	+		GFF4646	0.57	-0.1
3,064	+		GFF4646	0.57	-0.5
3,064	+		GFF4646	0.57	-1.3
3,065	-		GFF4646	0.57	-1.1
3,178	+		GFF4646	0.68	-0.7
3,178	+		GFF4646	0.68	-0.6
3,179	-		GFF4646	0.69	-0.2
3,410	-				+1.2
3,449	-				-0.5
3,454	+				-0.4
3,454	+				+0.2
3,454	+				-0.9
3,454	+				-1.6
3,454	+				+0.1
3,454	+				-0.4
3,454	+				+0.7
3,454	+				-1.3
3,454	+				+0.3
3,454	+				-0.3
3,454	+				+0.5
3,454	+				+0.5
3,454	+				+0.5
3,455	-				-1.3
3,455	-				-0.4
3,455	-				-0.9
3,455	-				+0.6
3,455	-				-0.0
3,455	-				-0.5
3,455	-				-0.5
3,455	-				-2.1
3,455	-				-0.5
3,455	-				-0.5
3,458	-				+1.9
3,458	-				+1.0
3,501	-				+1.3
3,514	+				+0.3
3,515	-				-0.5
3,522	+				+0.7
3,523	-				-0.8
3,523	-				-0.5
3,523	-				-2.0
3,526	+				-1.4
3,526	+				-0.7
3,527	-				+0.6
3,532	-				-0.4
3,597	+		GFF4647	0.12	+0.9
3,598	-		GFF4647	0.12	-0.7
3,598	-		GFF4647	0.12	-1.6
4,187	+		GFF4648	0.11	-1.1
4,187	+		GFF4648	0.11	+0.1
4,187	+		GFF4648	0.11	-0.1
4,187	+		GFF4648	0.11	-1.1
4,187	+		GFF4648	0.11	-0.1
4,187	+		GFF4648	0.11	+0.0
4,187	+		GFF4648	0.11	+0.9
4,187	+		GFF4648	0.11	+0.5
4,187	+		GFF4648	0.11	-0.9
4,188	-		GFF4648	0.11	-1.5
4,188	-		GFF4648	0.11	-0.4
4,188	-		GFF4648	0.11	+0.3
4,188	-		GFF4648	0.11	+0.9
4,188	-		GFF4648	0.11	+0.1
4,188	-		GFF4648	0.11	-0.3
4,577	+		GFF4648	0.37	-1.3
4,577	+		GFF4648	0.37	-0.2
4,578	-		GFF4648	0.37	-1.5
4,762	+		GFF4648	0.49	-0.5
4,762	+		GFF4648	0.49	+0.4
4,763	-		GFF4648	0.49	-0.8
4,763	-		GFF4648	0.49	+0.6
4,925	+		GFF4648	0.59	+0.0
4,926	-		GFF4648	0.59	-0.7
4,926	-		GFF4648	0.59	-1.0
4,926	-		GFF4648	0.59	-0.3