Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4602 and GFF4603 are separated by 26 nucleotides GFF4603 and GFF4604 are separated by 2 nucleotides GFF4604 and GFF4605 are separated by 29 nucleotides GFF4605 and GFF4606 are separated by 55 nucleotides
GFF4602 - 2-hydroxychromene-2-carboxylate isomerase family protein, at 244,163 to 244,759
GFF4602
GFF4603 - Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein, at 244,786 to 245,535
GFF4603
GFF4604 - TesB-like acyl-CoA thioesterase 2, at 245,538 to 246,374
GFF4604
GFF4605 - FIG00537210: hypothetical protein, at 246,404 to 247,309
GFF4605
GFF4606 - 3-ketoacyl-CoA thiolase [fadN-fadA-fadE operon] (EC 2.3.1.16), at 247,365 to 248,561
GFF4606
Position (kb) 245
246
247 Strain fitness (log2 ratio) -2
-1
0
1 at 244.575 kb on + strand, within GFF4602 at 244.575 kb on + strand, within GFF4602 at 244.575 kb on + strand, within GFF4602 at 244.575 kb on + strand, within GFF4602 at 244.575 kb on + strand, within GFF4602 at 244.575 kb on + strand, within GFF4602 at 244.576 kb on - strand, within GFF4602 at 244.576 kb on - strand, within GFF4602 at 244.576 kb on - strand, within GFF4602 at 244.576 kb on - strand, within GFF4602 at 244.576 kb on - strand, within GFF4602 at 244.710 kb on + strand at 244.711 kb on - strand at 244.711 kb on - strand at 244.738 kb on - strand at 244.862 kb on + strand, within GFF4603 at 244.862 kb on + strand, within GFF4603 at 244.862 kb on + strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 244.863 kb on - strand, within GFF4603 at 245.219 kb on + strand, within GFF4603 at 245.219 kb on + strand, within GFF4603 at 245.530 kb on - strand at 245.530 kb on - strand at 245.530 kb on - strand at 245.548 kb on + strand at 245.549 kb on - strand at 245.549 kb on - strand at 245.549 kb on - strand at 245.551 kb on + strand at 245.552 kb on - strand at 245.552 kb on - strand at 245.662 kb on + strand, within GFF4604 at 245.662 kb on + strand, within GFF4604 at 245.662 kb on + strand, within GFF4604 at 245.662 kb on + strand, within GFF4604 at 245.663 kb on - strand, within GFF4604 at 245.663 kb on - strand, within GFF4604 at 245.707 kb on + strand, within GFF4604 at 245.707 kb on + strand, within GFF4604 at 245.707 kb on + strand, within GFF4604 at 245.708 kb on - strand, within GFF4604 at 245.708 kb on - strand, within GFF4604 at 245.708 kb on - strand, within GFF4604 at 245.770 kb on + strand, within GFF4604 at 245.911 kb on + strand, within GFF4604 at 246.172 kb on + strand, within GFF4604 at 246.172 kb on + strand, within GFF4604 at 246.172 kb on + strand, within GFF4604 at 246.281 kb on - strand, within GFF4604 at 246.591 kb on + strand, within GFF4605 at 246.591 kb on + strand, within GFF4605 at 246.592 kb on - strand, within GFF4605 at 246.592 kb on - strand, within GFF4605 at 246.681 kb on + strand, within GFF4605 at 246.682 kb on - strand, within GFF4605 at 246.682 kb on - strand, within GFF4605 at 246.807 kb on + strand, within GFF4605 at 246.853 kb on - strand, within GFF4605 at 246.853 kb on - strand, within GFF4605 at 246.991 kb on - strand, within GFF4605 at 246.991 kb on - strand, within GFF4605 at 247.179 kb on + strand, within GFF4605 at 247.179 kb on + strand, within GFF4605 at 247.180 kb on - strand, within GFF4605 at 247.180 kb on - strand, within GFF4605 at 247.248 kb on + strand at 247.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 244,575 + GFF4602 0.69 -0.4 244,575 + GFF4602 0.69 -1.1 244,575 + GFF4602 0.69 -1.2 244,575 + GFF4602 0.69 +0.2 244,575 + GFF4602 0.69 -0.1 244,575 + GFF4602 0.69 +0.1 244,576 - GFF4602 0.69 -0.6 244,576 - GFF4602 0.69 +0.8 244,576 - GFF4602 0.69 +0.2 244,576 - GFF4602 0.69 -0.2 244,576 - GFF4602 0.69 -0.7 244,710 + +0.1 244,711 - -0.6 244,711 - +0.3 244,738 - -0.1 244,862 + GFF4603 0.10 -0.0 244,862 + GFF4603 0.10 -0.3 244,862 + GFF4603 0.10 +0.3 244,863 - GFF4603 0.10 -0.4 244,863 - GFF4603 0.10 +0.0 244,863 - GFF4603 0.10 +0.4 244,863 - GFF4603 0.10 -0.8 244,863 - GFF4603 0.10 +0.9 244,863 - GFF4603 0.10 -0.3 244,863 - GFF4603 0.10 -0.5 244,863 - GFF4603 0.10 -0.8 244,863 - GFF4603 0.10 +0.1 244,863 - GFF4603 0.10 +0.1 245,219 + GFF4603 0.58 -0.2 245,219 + GFF4603 0.58 -1.1 245,530 - -0.1 245,530 - -0.4 245,530 - -0.1 245,548 + -1.2 245,549 - -0.5 245,549 - -0.1 245,549 - -0.4 245,551 + +0.3 245,552 - -1.5 245,552 - -0.7 245,662 + GFF4604 0.15 +0.1 245,662 + GFF4604 0.15 -0.1 245,662 + GFF4604 0.15 -0.4 245,662 + GFF4604 0.15 +0.3 245,663 - GFF4604 0.15 +0.4 245,663 - GFF4604 0.15 -0.7 245,707 + GFF4604 0.20 +0.2 245,707 + GFF4604 0.20 -0.2 245,707 + GFF4604 0.20 +1.5 245,708 - GFF4604 0.20 -0.6 245,708 - GFF4604 0.20 -0.5 245,708 - GFF4604 0.20 +0.7 245,770 + GFF4604 0.28 -0.2 245,911 + GFF4604 0.45 +0.1 246,172 + GFF4604 0.76 +0.4 246,172 + GFF4604 0.76 +0.5 246,172 + GFF4604 0.76 -1.4 246,281 - GFF4604 0.89 -1.1 246,591 + GFF4605 0.21 -0.3 246,591 + GFF4605 0.21 +0.3 246,592 - GFF4605 0.21 +0.1 246,592 - GFF4605 0.21 -0.1 246,681 + GFF4605 0.31 +0.9 246,682 - GFF4605 0.31 -0.1 246,682 - GFF4605 0.31 +0.5 246,807 + GFF4605 0.44 -2.6 246,853 - GFF4605 0.50 -0.7 246,853 - GFF4605 0.50 +0.2 246,991 - GFF4605 0.65 -0.3 246,991 - GFF4605 0.65 +0.8 247,179 + GFF4605 0.86 -0.1 247,179 + GFF4605 0.86 -1.7 247,180 - GFF4605 0.86 -0.4 247,180 - GFF4605 0.86 +1.3 247,248 + -0.4 247,249 - +0.1
Or see this region's nucleotide sequence