Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4305 and GFF4306 overlap by 4 nucleotides GFF4306 and GFF4307 are separated by 1 nucleotides GFF4307 and GFF4308 are separated by 48 nucleotides
GFF4305 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 225,374 to 226,198
GFF4305
GFF4306 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 226,195 to 227,067
GFF4306
GFF4307 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 227,069 to 228,109
GFF4307
GFF4308 - hypothetical protein, at 228,158 to 229,363
GFF4308
Position (kb) 227
228
229 Strain fitness (log2 ratio) -2
-1
0
1 at 226.342 kb on + strand, within GFF4306 at 226.343 kb on - strand, within GFF4306 at 226.343 kb on - strand, within GFF4306 at 226.343 kb on - strand, within GFF4306 at 226.345 kb on + strand, within GFF4306 at 226.753 kb on + strand, within GFF4306 at 226.961 kb on - strand, within GFF4306 at 227.195 kb on + strand, within GFF4307 at 227.319 kb on - strand, within GFF4307 at 227.357 kb on + strand, within GFF4307 at 227.358 kb on - strand, within GFF4307 at 227.786 kb on + strand, within GFF4307 at 227.787 kb on - strand, within GFF4307 at 227.792 kb on + strand, within GFF4307 at 227.793 kb on - strand, within GFF4307 at 227.813 kb on + strand, within GFF4307 at 227.814 kb on - strand, within GFF4307 at 228.142 kb on - strand at 228.342 kb on - strand, within GFF4308 at 228.698 kb on + strand, within GFF4308 at 228.699 kb on - strand, within GFF4308 at 228.704 kb on + strand, within GFF4308 at 228.704 kb on + strand, within GFF4308 at 228.864 kb on + strand, within GFF4308 at 228.864 kb on + strand, within GFF4308 at 228.875 kb on + strand, within GFF4308 at 228.875 kb on + strand, within GFF4308 at 229.022 kb on + strand, within GFF4308 at 229.022 kb on - strand, within GFF4308
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 226,342 + GFF4306 0.17 -1.6 226,343 - GFF4306 0.17 +0.4 226,343 - GFF4306 0.17 -1.7 226,343 - GFF4306 0.17 -0.2 226,345 + GFF4306 0.17 +0.2 226,753 + GFF4306 0.64 -0.2 226,961 - GFF4306 0.88 -2.3 227,195 + GFF4307 0.12 -0.1 227,319 - GFF4307 0.24 -0.1 227,357 + GFF4307 0.28 -0.4 227,358 - GFF4307 0.28 -0.7 227,786 + GFF4307 0.69 +0.6 227,787 - GFF4307 0.69 -0.4 227,792 + GFF4307 0.69 +0.7 227,793 - GFF4307 0.70 -0.0 227,813 + GFF4307 0.71 -2.6 227,814 - GFF4307 0.72 -1.7 228,142 - -0.4 228,342 - GFF4308 0.15 +1.1 228,698 + GFF4308 0.45 -2.7 228,699 - GFF4308 0.45 -0.2 228,704 + GFF4308 0.45 +0.0 228,704 + GFF4308 0.45 +0.1 228,864 + GFF4308 0.59 +1.0 228,864 + GFF4308 0.59 -0.1 228,875 + GFF4308 0.59 -0.7 228,875 + GFF4308 0.59 +0.5 229,022 + GFF4308 0.72 -0.1 229,022 - GFF4308 0.72 +0.2
Or see this region's nucleotide sequence