Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3982 and GFF3983 overlap by 4 nucleotides GFF3983 and GFF3984 overlap by 4 nucleotides GFF3984 and GFF3985 overlap by 11 nucleotides
GFF3982 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 185,550 to 186,605
GFF3982
GFF3983 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 186,602 to 188,389
GFF3983
GFF3984 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 188,386 to 189,117
GFF3984
GFF3985 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 189,107 to 190,696
GFF3985
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -2
-1
0
1 at 185.608 kb on - strand at 186.300 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.367 kb on - strand, within GFF3982 at 187.076 kb on + strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.079 kb on + strand, within GFF3983 at 187.080 kb on - strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.272 kb on - strand, within GFF3983 at 187.331 kb on + strand, within GFF3983 at 188.385 kb on + strand at 188.386 kb on - strand at 188.746 kb on + strand, within GFF3984 at 189.007 kb on + strand, within GFF3984 at 189.008 kb on - strand, within GFF3984 at 189.008 kb on - strand, within GFF3984 at 189.085 kb on + strand at 189.086 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 185,608 - -0.8 186,300 + GFF3982 0.71 -1.9 186,363 + GFF3982 0.77 -0.4 186,363 + GFF3982 0.77 -1.8 186,363 + GFF3982 0.77 -0.4 186,363 + GFF3982 0.77 +0.2 186,363 + GFF3982 0.77 -1.7 186,363 + GFF3982 0.77 +0.9 186,364 - GFF3982 0.77 -0.1 186,364 - GFF3982 0.77 -1.6 186,367 - GFF3982 0.77 +0.9 187,076 + GFF3983 0.27 +0.2 187,077 - GFF3983 0.27 +0.2 187,077 - GFF3983 0.27 +0.3 187,077 - GFF3983 0.27 +0.4 187,079 + GFF3983 0.27 +0.2 187,080 - GFF3983 0.27 -1.6 187,271 + GFF3983 0.37 +0.0 187,271 + GFF3983 0.37 -0.1 187,272 - GFF3983 0.37 +1.0 187,331 + GFF3983 0.41 -0.8 188,385 + +0.2 188,386 - -0.8 188,746 + GFF3984 0.49 -0.1 189,007 + GFF3984 0.85 -0.9 189,008 - GFF3984 0.85 -0.7 189,008 - GFF3984 0.85 -0.6 189,085 + -0.9 189,086 - -0.2
Or see this region's nucleotide sequence