Strain Fitness in Variovorax sp. SCN45 around GFF3945

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3943 and GFF3944 overlap by 4 nucleotidesGFF3944 and GFF3945 are separated by 123 nucleotidesGFF3945 and GFF3946 are separated by 13 nucleotides GFF3943 - Poly(A) polymerase (EC 2.7.7.19), at 146,504 to 148,135 GFF3943 GFF3944 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 148,132 to 148,770 GFF3944 GFF3945 - Phosphate transport regulator (distant homolog of PhoU), at 148,894 to 149,538 GFF3945 GFF3946 - Probable low-affinity inorganic phosphate transporter, at 149,552 to 150,562 GFF3946 Position (kb) 148 149 150Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 148.820 kb on + strandat 148.820 kb on + strandat 148.820 kb on + strandat 148.820 kb on + strandat 148.821 kb on - strandat 148.821 kb on - strandat 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.002 kb on + strand, within GFF3945at 149.003 kb on - strand, within GFF3945at 149.003 kb on - strand, within GFF3945at 149.003 kb on - strand, within GFF3945at 149.029 kb on + strand, within GFF3945at 149.029 kb on + strand, within GFF3945at 149.029 kb on + strand, within GFF3945at 149.029 kb on + strand, within GFF3945at 149.029 kb on + strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.030 kb on - strand, within GFF3945at 149.206 kb on + strand, within GFF3945at 149.207 kb on - strand, within GFF3945at 149.207 kb on - strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.458 kb on + strand, within GFF3945at 149.459 kb on - strand, within GFF3945at 149.459 kb on - strand, within GFF3945at 149.459 kb on - strand, within GFF3945at 149.459 kb on - strand, within GFF3945at 149.459 kb on - strand, within GFF3945at 149.459 kb on - strand, within GFF3945at 149.586 kb on + strandat 149.586 kb on + strandat 149.586 kb on + strandat 149.586 kb on + strandat 149.586 kb on + strandat 149.586 kb on + strandat 149.586 kb on + strandat 149.587 kb on - strandat 149.587 kb on - strandat 149.587 kb on - strandat 149.587 kb on - strandat 149.587 kb on - strandat 149.856 kb on - strand, within GFF3946at 149.856 kb on - strand, within GFF3946at 149.856 kb on - strand, within GFF3946at 149.856 kb on - strand, within GFF3946at 149.856 kb on - strand, within GFF3946at 149.858 kb on + strand, within GFF3946at 149.858 kb on + strand, within GFF3946at 149.858 kb on + strand, within GFF3946at 149.858 kb on + strand, within GFF3946at 149.858 kb on + strand, within GFF3946at 149.858 kb on + strand, within GFF3946at 149.858 kb on + strand, within GFF3946at 149.859 kb on - strand, within GFF3946at 149.859 kb on - strand, within GFF3946at 149.859 kb on - strand, within GFF3946at 150.110 kb on + strand, within GFF3946at 150.110 kb on + strand, within GFF3946at 150.110 kb on + strand, within GFF3946at 150.110 kb on + strand, within GFF3946at 150.111 kb on - strand, within GFF3946at 150.111 kb on - strand, within GFF3946at 150.111 kb on - strand, within GFF3946at 150.111 kb on - strand, within GFF3946at 150.185 kb on + strand, within GFF3946at 150.185 kb on + strand, within GFF3946at 150.186 kb on - strand, within GFF3946at 150.186 kb on - strand, within GFF3946at 150.186 kb on - strand, within GFF3946at 150.236 kb on + strand, within GFF3946at 150.409 kb on + strand, within GFF3946at 150.536 kb on + strandat 150.536 kb on + strandat 150.536 kb on + strandat 150.537 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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148,820 + -2.4
148,820 + -1.6
148,820 + +0.3
148,820 + -2.1
148,821 - -1.0
148,821 - +3.1
149,002 + GFF3945 0.17 -1.1
149,002 + GFF3945 0.17 -0.9
149,002 + GFF3945 0.17 -0.7
149,002 + GFF3945 0.17 -1.6
149,002 + GFF3945 0.17 -0.1
149,002 + GFF3945 0.17 -0.8
149,002 + GFF3945 0.17 -0.9
149,002 + GFF3945 0.17 -0.4
149,002 + GFF3945 0.17 -0.1
149,003 - GFF3945 0.17 -0.7
149,003 - GFF3945 0.17 -0.6
149,003 - GFF3945 0.17 -0.8
149,029 + GFF3945 0.21 -0.5
149,029 + GFF3945 0.21 -0.8
149,029 + GFF3945 0.21 +1.5
149,029 + GFF3945 0.21 +0.7
149,029 + GFF3945 0.21 -1.6
149,030 - GFF3945 0.21 +1.0
149,030 - GFF3945 0.21 -0.8
149,030 - GFF3945 0.21 -0.3
149,030 - GFF3945 0.21 +0.1
149,030 - GFF3945 0.21 +0.2
149,030 - GFF3945 0.21 +0.2
149,030 - GFF3945 0.21 -0.8
149,030 - GFF3945 0.21 -2.9
149,030 - GFF3945 0.21 -2.1
149,030 - GFF3945 0.21 -0.2
149,030 - GFF3945 0.21 -1.3
149,206 + GFF3945 0.48 -1.9
149,207 - GFF3945 0.49 +0.3
149,207 - GFF3945 0.49 -1.9
149,458 + GFF3945 0.87 -0.9
149,458 + GFF3945 0.87 +0.8
149,458 + GFF3945 0.87 +0.5
149,458 + GFF3945 0.87 -0.0
149,458 + GFF3945 0.87 -0.9
149,458 + GFF3945 0.87 -1.0
149,458 + GFF3945 0.87 -0.1
149,458 + GFF3945 0.87 -0.4
149,459 - GFF3945 0.88 -0.2
149,459 - GFF3945 0.88 +0.5
149,459 - GFF3945 0.88 -0.5
149,459 - GFF3945 0.88 -1.2
149,459 - GFF3945 0.88 -1.1
149,459 - GFF3945 0.88 -1.3
149,586 + -0.8
149,586 + -0.6
149,586 + -0.7
149,586 + +1.2
149,586 + -0.9
149,586 + -0.8
149,586 + -0.0
149,587 - -0.1
149,587 - -0.3
149,587 - -0.9
149,587 - -0.3
149,587 - -1.0
149,856 - GFF3946 0.30 -1.1
149,856 - GFF3946 0.30 +0.5
149,856 - GFF3946 0.30 -0.4
149,856 - GFF3946 0.30 -2.9
149,856 - GFF3946 0.30 -0.8
149,858 + GFF3946 0.30 -2.0
149,858 + GFF3946 0.30 -0.6
149,858 + GFF3946 0.30 -0.7
149,858 + GFF3946 0.30 -0.7
149,858 + GFF3946 0.30 -0.4
149,858 + GFF3946 0.30 -1.2
149,858 + GFF3946 0.30 -0.8
149,859 - GFF3946 0.30 -0.5
149,859 - GFF3946 0.30 -0.5
149,859 - GFF3946 0.30 -0.2
150,110 + GFF3946 0.55 -0.6
150,110 + GFF3946 0.55 -0.5
150,110 + GFF3946 0.55 +0.8
150,110 + GFF3946 0.55 +0.9
150,111 - GFF3946 0.55 -0.3
150,111 - GFF3946 0.55 -0.9
150,111 - GFF3946 0.55 -1.1
150,111 - GFF3946 0.55 -0.3
150,185 + GFF3946 0.63 -0.7
150,185 + GFF3946 0.63 +1.5
150,186 - GFF3946 0.63 -0.4
150,186 - GFF3946 0.63 -0.7
150,186 - GFF3946 0.63 -0.2
150,236 + GFF3946 0.68 -0.8
150,409 + GFF3946 0.85 -0.6
150,536 + -0.5
150,536 + +0.8
150,536 + +0.5
150,537 - -0.3

Or see this region's nucleotide sequence