Strain Fitness in Variovorax sp. SCN45 around GFF3935

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
137,837	+				+0.1
137,901	-		GFF3934	0.23	+0.0
137,901	-		GFF3934	0.23	+0.0
137,901	-		GFF3934	0.23	+0.1
137,901	-		GFF3934	0.23	-0.5
137,901	-		GFF3934	0.23	-1.2
137,901	-		GFF3934	0.23	-1.8
138,841	+		GFF3935	0.48	-1.4
138,841	+		GFF3935	0.48	-1.5
138,841	+		GFF3935	0.48	-0.8
138,841	+		GFF3935	0.48	-2.1
138,841	+		GFF3935	0.48	-0.6
138,842	-		GFF3935	0.48	+0.4
138,842	-		GFF3935	0.48	-0.7
138,842	-		GFF3935	0.48	+0.5
138,842	-		GFF3935	0.48	-0.6
138,842	-		GFF3935	0.48	+1.5
138,842	-		GFF3935	0.48	-0.4
138,842	-		GFF3935	0.48	-0.6
138,842	-		GFF3935	0.48	-0.8
138,842	-		GFF3935	0.48	-0.5
138,862	+		GFF3935	0.49	-0.4
138,862	+		GFF3935	0.49	+0.3
138,863	-		GFF3935	0.49	+0.9
138,863	-		GFF3935	0.49	-1.3
138,863	-		GFF3935	0.49	-1.1
138,965	+		GFF3935	0.57	-0.5
138,965	+		GFF3935	0.57	-1.1
138,966	-		GFF3935	0.57	-0.5
139,144	+		GFF3935	0.71	-0.6
139,145	-		GFF3935	0.71	-0.7
139,228	+		GFF3935	0.77	-0.7
139,228	+		GFF3935	0.77	+1.4
139,228	+		GFF3935	0.77	-1.1
139,228	+		GFF3935	0.77	+0.3
139,228	+		GFF3935	0.77	-0.4
139,229	-		GFF3935	0.78	-1.0
139,229	-		GFF3935	0.78	-0.1
139,229	-		GFF3935	0.78	-0.4
139,229	-		GFF3935	0.78	+1.4
139,364	-		GFF3935	0.88	-2.6
139,364	-		GFF3935	0.88	+0.2
139,364	-		GFF3935	0.88	+0.7
139,464	+				-0.1
139,465	-				-0.2
139,465	-				-0.9
139,721	+				-0.7
139,721	+				-0.7
139,722	-				-1.9
139,722	-				-0.1
139,722	-				-0.6
139,722	-				-0.1
139,722	-				-0.4
140,166	-		GFF3936	0.37	+1.5
140,246	+		GFF3936	0.42	+0.1
140,246	+		GFF3936	0.42	-0.7
140,247	-		GFF3936	0.42	-0.1
140,259	+		GFF3936	0.43	-1.3
140,259	+		GFF3936	0.43	-1.5
140,259	+		GFF3936	0.43	-0.1
140,259	+		GFF3936	0.43	+0.5
140,259	+		GFF3936	0.43	+0.1
140,259	+		GFF3936	0.43	+0.0
140,260	-		GFF3936	0.43	-0.1
140,260	-		GFF3936	0.43	-0.9
140,318	+		GFF3936	0.46	-0.1
140,319	-		GFF3936	0.46	-0.3
140,319	-		GFF3936	0.46	+0.1
140,319	-		GFF3936	0.46	-0.4
140,319	-		GFF3936	0.46	-0.1
140,319	-		GFF3936	0.46	-0.9
140,319	-		GFF3936	0.46	-0.5
140,372	+		GFF3936	0.50	-0.7
140,372	+		GFF3936	0.50	-0.3
140,372	+		GFF3936	0.50	-0.4
140,372	+		GFF3936	0.50	+0.2
140,372	+		GFF3936	0.50	-0.4
140,373	-		GFF3936	0.50	+0.5
140,373	-		GFF3936	0.50	-1.7
140,373	-		GFF3936	0.50	-1.1
140,373	-		GFF3936	0.50	+0.1
140,373	-		GFF3936	0.50	-1.8
140,373	-		GFF3936	0.50	-0.2