Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3743 and GFF3744 are separated by 51 nucleotides GFF3744 and GFF3745 are separated by 25 nucleotides GFF3745 and GFF3746 are separated by 10 nucleotides GFF3746 and GFF3747 overlap by 4 nucleotides
GFF3743 - Twitching motility protein PilT, at 239,251 to 240,294
GFF3743
GFF3744 - Pyridoxal phosphate-containing protein YggS, at 240,346 to 241,047
GFF3744
GFF3745 - Uncharacterized UPF0721 integral membrane protein, at 241,073 to 241,813
GFF3745
GFF3746 - Probable NADH dehydrogenase/NAD(P)H nitroreductase, at 241,824 to 242,414
GFF3746
GFF3747 - putative esterase, at 242,411 to 243,253
GFF3747
Position (kb) 241
242 Strain fitness (log2 ratio) -2
-1
0
1
2 at 240.107 kb on + strand, within GFF3743 at 240.107 kb on + strand, within GFF3743 at 240.107 kb on + strand, within GFF3743 at 240.107 kb on + strand, within GFF3743 at 240.107 kb on + strand, within GFF3743 at 240.107 kb on + strand, within GFF3743 at 240.108 kb on - strand, within GFF3743 at 240.140 kb on + strand, within GFF3743 at 240.140 kb on + strand, within GFF3743 at 240.140 kb on + strand, within GFF3743 at 240.140 kb on + strand, within GFF3743 at 240.140 kb on + strand, within GFF3743 at 240.141 kb on - strand, within GFF3743 at 240.346 kb on - strand at 240.346 kb on - strand at 240.377 kb on + strand at 240.377 kb on + strand at 240.377 kb on + strand at 240.378 kb on - strand at 240.378 kb on - strand at 240.378 kb on - strand at 240.378 kb on - strand at 240.378 kb on - strand at 240.378 kb on - strand at 240.378 kb on - strand at 241.173 kb on + strand, within GFF3745 at 241.173 kb on + strand, within GFF3745 at 241.174 kb on - strand, within GFF3745 at 241.242 kb on + strand, within GFF3745 at 241.242 kb on + strand, within GFF3745 at 241.243 kb on - strand, within GFF3745 at 241.243 kb on - strand, within GFF3745 at 241.372 kb on - strand, within GFF3745 at 241.380 kb on + strand, within GFF3745 at 241.380 kb on + strand, within GFF3745 at 241.380 kb on + strand, within GFF3745 at 241.650 kb on + strand, within GFF3745 at 241.650 kb on + strand, within GFF3745 at 241.651 kb on - strand, within GFF3745 at 241.651 kb on - strand, within GFF3745 at 241.651 kb on - strand, within GFF3745 at 242.024 kb on - strand, within GFF3746 at 242.314 kb on + strand, within GFF3746 at 242.314 kb on + strand, within GFF3746 at 242.315 kb on - strand, within GFF3746 at 242.315 kb on - strand, within GFF3746 at 242.315 kb on - strand, within GFF3746 at 242.571 kb on + strand, within GFF3747 at 242.571 kb on + strand, within GFF3747 at 242.571 kb on + strand, within GFF3747 at 242.572 kb on - strand, within GFF3747 at 242.733 kb on + strand, within GFF3747 at 242.734 kb on - strand, within GFF3747
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 240,107 + GFF3743 0.82 -0.3 240,107 + GFF3743 0.82 -1.9 240,107 + GFF3743 0.82 -0.9 240,107 + GFF3743 0.82 -0.8 240,107 + GFF3743 0.82 -1.9 240,107 + GFF3743 0.82 -2.0 240,108 - GFF3743 0.82 -2.1 240,140 + GFF3743 0.85 -1.2 240,140 + GFF3743 0.85 +0.4 240,140 + GFF3743 0.85 -0.1 240,140 + GFF3743 0.85 +0.7 240,140 + GFF3743 0.85 -1.3 240,141 - GFF3743 0.85 +0.2 240,346 - -0.2 240,346 - -0.7 240,377 + -0.6 240,377 + +0.1 240,377 + -0.4 240,378 - -0.9 240,378 - -0.5 240,378 - -0.6 240,378 - -1.7 240,378 - +0.5 240,378 - -0.3 240,378 - -0.0 241,173 + GFF3745 0.13 -0.5 241,173 + GFF3745 0.13 -0.2 241,174 - GFF3745 0.14 -0.6 241,242 + GFF3745 0.23 +0.0 241,242 + GFF3745 0.23 -0.9 241,243 - GFF3745 0.23 -0.3 241,243 - GFF3745 0.23 +0.6 241,372 - GFF3745 0.40 +0.0 241,380 + GFF3745 0.41 -0.1 241,380 + GFF3745 0.41 +0.1 241,380 + GFF3745 0.41 +0.2 241,650 + GFF3745 0.78 +0.2 241,650 + GFF3745 0.78 -0.6 241,651 - GFF3745 0.78 -0.8 241,651 - GFF3745 0.78 +0.5 241,651 - GFF3745 0.78 -0.2 242,024 - GFF3746 0.34 -0.9 242,314 + GFF3746 0.83 +0.1 242,314 + GFF3746 0.83 +1.1 242,315 - GFF3746 0.83 -2.5 242,315 - GFF3746 0.83 -0.3 242,315 - GFF3746 0.83 +1.3 242,571 + GFF3747 0.19 -0.1 242,571 + GFF3747 0.19 -0.3 242,571 + GFF3747 0.19 -0.1 242,572 - GFF3747 0.19 +1.7 242,733 + GFF3747 0.38 +2.0 242,734 - GFF3747 0.38 +0.1
Or see this region's nucleotide sequence