Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3082 and GFF3084 are separated by 43 nucleotides GFF3084 and GFF3083 overlap by 2 nucleotides GFF3083 and GFF3085 are separated by 110 nucleotides GFF3085 and GFF3086 are separated by 253 nucleotides
GFF3082 - hypothetical protein, at 514,121 to 514,561
GFF3082
GFF3084 - no description, at 514,605 to 514,880
GFF3084
GFF3083 - no description, at 514,879 to 515,016
GFF3083
GFF3085 - no description, at 515,127 to 515,399
GFF3085
GFF3086 - DNA-binding protein HU-beta, at 515,653 to 516,063
GFF3086
Position (kb)
515
516 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 514.145 kb on + strand at 514.145 kb on + strand at 514.146 kb on - strand at 514.230 kb on + strand, within GFF3082 at 514.230 kb on + strand, within GFF3082 at 514.230 kb on + strand, within GFF3082 at 514.230 kb on + strand, within GFF3082 at 514.266 kb on + strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.537 kb on + strand at 514.538 kb on - strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.576 kb on + strand at 514.576 kb on + strand at 514.576 kb on + strand at 514.576 kb on + strand at 514.577 kb on - strand at 514.577 kb on - strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.586 kb on - strand at 514.586 kb on - strand at 514.586 kb on - strand at 514.606 kb on + strand at 514.780 kb on + strand, within GFF3084 at 514.780 kb on + strand, within GFF3084 at 514.780 kb on + strand, within GFF3084 at 514.780 kb on + strand, within GFF3084 at 514.780 kb on + strand, within GFF3084 at 514.780 kb on + strand, within GFF3084 at 514.781 kb on - strand, within GFF3084 at 514.781 kb on - strand, within GFF3084 at 514.781 kb on - strand, within GFF3084 at 514.781 kb on - strand, within GFF3084 at 514.781 kb on - strand, within GFF3084 at 514.781 kb on - strand, within GFF3084 at 515.098 kb on + strand at 515.098 kb on + strand at 515.272 kb on + strand, within GFF3085 at 515.272 kb on + strand, within GFF3085 at 515.272 kb on + strand, within GFF3085 at 515.465 kb on + strand at 515.465 kb on + strand at 515.466 kb on - strand at 515.466 kb on - strand at 515.466 kb on - strand at 515.479 kb on + strand at 515.480 kb on - strand at 515.491 kb on + strand at 515.491 kb on + strand at 515.492 kb on - strand at 515.492 kb on - strand at 515.492 kb on - strand at 515.654 kb on + strand at 515.654 kb on + strand at 515.655 kb on - strand at 515.655 kb on - strand at 515.655 kb on - strand at 516.073 kb on + strand at 516.074 kb on - strand at 516.074 kb on - strand at 516.074 kb on - strand at 516.074 kb on - strand at 516.074 kb on - strand at 516.074 kb on - strand at 516.076 kb on + strand at 516.076 kb on + strand at 516.077 kb on - strand at 516.077 kb on - strand at 516.077 kb on - strand at 516.099 kb on + strand at 516.119 kb on + strand at 516.119 kb on + strand at 516.145 kb on - strand at 516.164 kb on + strand at 516.164 kb on + strand at 516.164 kb on + strand at 516.164 kb on + strand at 516.165 kb on - strand at 516.165 kb on - strand at 516.165 kb on - strand at 516.165 kb on - strand at 516.165 kb on - strand at 516.168 kb on + strand at 516.168 kb on + strand at 516.168 kb on + strand at 516.168 kb on + strand at 516.168 kb on + strand at 516.168 kb on + strand at 516.168 kb on + strand at 516.168 kb on + strand at 516.169 kb on - strand at 516.169 kb on - strand at 516.169 kb on - strand at 516.169 kb on - strand at 516.169 kb on - strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.181 kb on + strand at 516.182 kb on - strand at 516.182 kb on - strand at 516.255 kb on + strand at 516.295 kb on + strand at 516.295 kb on + strand at 516.295 kb on + strand at 516.295 kb on + strand at 516.296 kb on - strand at 516.364 kb on + strand at 516.364 kb on + strand at 516.364 kb on + strand at 516.364 kb on + strand at 516.364 kb on + strand at 516.364 kb on + strand at 516.364 kb on + strand at 516.364 kb on + strand at 516.365 kb on - strand at 516.365 kb on - strand at 516.365 kb on - strand at 516.365 kb on - strand at 516.365 kb on - strand at 516.392 kb on - strand at 516.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 514,145 + -0.3 514,145 + -0.1 514,146 - -0.1 514,230 + GFF3082 0.25 -0.5 514,230 + GFF3082 0.25 +0.2 514,230 + GFF3082 0.25 +0.4 514,230 + GFF3082 0.25 -0.8 514,266 + GFF3082 0.33 -0.6 514,267 - GFF3082 0.33 -1.5 514,267 - GFF3082 0.33 +0.2 514,267 - GFF3082 0.33 -0.7 514,267 - GFF3082 0.33 +0.4 514,537 + -0.0 514,538 - -3.1 514,561 + +0.3 514,561 + -0.6 514,561 + -0.0 514,576 + -0.9 514,576 + -1.3 514,576 + -1.1 514,576 + +0.1 514,577 - +0.1 514,577 - -1.0 514,585 + -1.7 514,585 + +1.3 514,585 + -0.8 514,585 + -0.5 514,585 + -1.1 514,586 - +0.5 514,586 - +0.2 514,586 - -1.2 514,606 + -1.1 514,780 + GFF3084 0.63 -0.1 514,780 + GFF3084 0.63 +0.5 514,780 + GFF3084 0.63 +0.6 514,780 + GFF3084 0.63 +0.7 514,780 + GFF3084 0.63 -0.1 514,780 + GFF3084 0.63 +1.8 514,781 - GFF3084 0.64 -0.1 514,781 - GFF3084 0.64 -1.7 514,781 - GFF3084 0.64 -1.7 514,781 - GFF3084 0.64 -0.9 514,781 - GFF3084 0.64 -0.5 514,781 - GFF3084 0.64 -0.0 515,098 + -0.2 515,098 + +0.1 515,272 + GFF3085 0.53 +0.2 515,272 + GFF3085 0.53 +0.7 515,272 + GFF3085 0.53 +0.7 515,465 + -0.6 515,465 + -0.3 515,466 - -0.8 515,466 - -1.4 515,466 - -0.5 515,479 + -0.2 515,480 - -0.5 515,491 + -0.6 515,491 + -0.9 515,492 - +0.5 515,492 - -1.5 515,492 - -1.1 515,654 + +0.0 515,654 + -0.2 515,655 - -0.1 515,655 - +0.3 515,655 - -0.3 516,073 + +1.9 516,074 - +0.2 516,074 - -0.8 516,074 - -0.4 516,074 - -1.0 516,074 - -1.5 516,074 - -1.1 516,076 + +1.7 516,076 + -1.0 516,077 - -0.1 516,077 - +0.2 516,077 - -0.4 516,099 + +0.5 516,119 + +0.5 516,119 + -0.2 516,145 - +0.8 516,164 + -0.2 516,164 + +0.3 516,164 + +1.0 516,164 + +0.1 516,165 - -1.5 516,165 - -2.9 516,165 - +0.4 516,165 - -0.3 516,165 - -0.5 516,168 + +1.8 516,168 + -0.8 516,168 + -1.0 516,168 + -0.5 516,168 + -0.1 516,168 + +0.2 516,168 + -0.1 516,168 + -0.3 516,169 - -0.4 516,169 - +1.0 516,169 - +0.1 516,169 - +0.5 516,169 - -0.5 516,181 + +0.1 516,181 + +0.8 516,181 + -0.2 516,181 + -1.2 516,181 + +0.4 516,181 + -0.6 516,181 + +0.9 516,181 + -0.9 516,181 + -1.1 516,181 + -0.5 516,182 - -1.0 516,182 - +0.7 516,255 + -0.8 516,295 + -0.7 516,295 + -0.2 516,295 + +0.5 516,295 + -0.3 516,296 - -0.1 516,364 + +0.3 516,364 + +0.1 516,364 + +0.3 516,364 + +0.2 516,364 + -0.6 516,364 + -0.7 516,364 + +0.9 516,364 + +0.8 516,365 - -0.6 516,365 - +0.2 516,365 - -0.8 516,365 - -0.3 516,365 - +0.5 516,392 - -0.4 516,392 - +0.2
Or see this region's nucleotide sequence