Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2991 and GFF2992 are separated by 211 nucleotides GFF2992 and GFF2993 are separated by 8 nucleotides
GFF2991 - BUG/TctC family periplasmic protein, at 421,945 to 422,904
GFF2991
GFF2992 - BUG/TctC family periplasmic protein, at 423,116 to 424,105
GFF2992
GFF2993 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 424,114 to 425,544
GFF2993
Position (kb) 423
424
425 Strain fitness (log2 ratio) -2
-1
0
1
2 at 422.591 kb on + strand, within GFF2991 at 422.591 kb on + strand, within GFF2991 at 422.684 kb on + strand, within GFF2991 at 422.684 kb on + strand, within GFF2991 at 422.684 kb on + strand, within GFF2991 at 422.684 kb on + strand, within GFF2991 at 422.684 kb on + strand, within GFF2991 at 422.685 kb on - strand, within GFF2991 at 422.685 kb on - strand, within GFF2991 at 422.685 kb on - strand, within GFF2991 at 423.072 kb on + strand at 423.072 kb on + strand at 423.072 kb on + strand at 423.073 kb on - strand at 423.073 kb on - strand at 423.117 kb on + strand at 423.117 kb on + strand at 423.117 kb on + strand at 423.128 kb on - strand at 423.333 kb on + strand, within GFF2992 at 423.334 kb on - strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.625 kb on - strand, within GFF2992 at 423.625 kb on - strand, within GFF2992 at 423.798 kb on + strand, within GFF2992 at 423.798 kb on + strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 424.277 kb on + strand, within GFF2993 at 424.278 kb on - strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.466 kb on - strand, within GFF2993 at 424.466 kb on - strand, within GFF2993 at 425.090 kb on + strand, within GFF2993 at 425.090 kb on + strand, within GFF2993 at 425.091 kb on - strand, within GFF2993 at 425.091 kb on - strand, within GFF2993
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 422,591 + GFF2991 0.67 +0.1 422,591 + GFF2991 0.67 -0.8 422,684 + GFF2991 0.77 -0.3 422,684 + GFF2991 0.77 -0.3 422,684 + GFF2991 0.77 -0.5 422,684 + GFF2991 0.77 -0.1 422,684 + GFF2991 0.77 +1.8 422,685 - GFF2991 0.77 +1.2 422,685 - GFF2991 0.77 -0.9 422,685 - GFF2991 0.77 -0.5 423,072 + +0.4 423,072 + +0.9 423,072 + -0.4 423,073 - -2.0 423,073 - -0.1 423,117 + -0.1 423,117 + -0.3 423,117 + +1.5 423,128 - -0.6 423,333 + GFF2992 0.22 -2.7 423,334 - GFF2992 0.22 -0.5 423,537 + GFF2992 0.43 -1.2 423,537 + GFF2992 0.43 +1.4 423,537 + GFF2992 0.43 -0.2 423,537 + GFF2992 0.43 -1.9 423,537 + GFF2992 0.43 -0.9 423,537 + GFF2992 0.43 +0.1 423,537 + GFF2992 0.43 -0.2 423,537 + GFF2992 0.43 -0.1 423,538 - GFF2992 0.43 -0.1 423,538 - GFF2992 0.43 -0.7 423,538 - GFF2992 0.43 -0.7 423,538 - GFF2992 0.43 -2.6 423,544 + GFF2992 0.43 +0.2 423,544 + GFF2992 0.43 -0.6 423,544 + GFF2992 0.43 -0.4 423,544 + GFF2992 0.43 -0.1 423,544 + GFF2992 0.43 -0.4 423,625 - GFF2992 0.51 +0.6 423,625 - GFF2992 0.51 -0.5 423,798 + GFF2992 0.69 +1.5 423,798 + GFF2992 0.69 -0.1 423,799 - GFF2992 0.69 -0.2 423,799 - GFF2992 0.69 -0.8 423,799 - GFF2992 0.69 +1.1 423,799 - GFF2992 0.69 -1.1 424,277 + GFF2993 0.11 -2.1 424,278 - GFF2993 0.11 -1.5 424,465 + GFF2993 0.25 -0.5 424,465 + GFF2993 0.25 -0.6 424,465 + GFF2993 0.25 +0.4 424,465 + GFF2993 0.25 +0.1 424,465 + GFF2993 0.25 -0.1 424,465 + GFF2993 0.25 -0.2 424,466 - GFF2993 0.25 +0.3 424,466 - GFF2993 0.25 +0.2 425,090 + GFF2993 0.68 -0.5 425,090 + GFF2993 0.68 -2.3 425,091 - GFF2993 0.68 -1.0 425,091 - GFF2993 0.68 -0.6
Or see this region's nucleotide sequence