Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2958 and GFF2959 overlap by 4 nucleotides GFF2959 and GFF2960 are separated by 64 nucleotides GFF2960 and GFF2961 are separated by 103 nucleotides
GFF2958 - hypothetical protein, at 385,847 to 387,583
GFF2958
GFF2959 - Uncharacterized protein YfaP, at 387,580 to 388,395
GFF2959
GFF2960 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 388,460 to 389,023
GFF2960
GFF2961 - CzcABC family efflux RND transporter, transmembrane protein, at 389,127 to 392,348
GFF2961
Position (kb) 387
388
389 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 386.721 kb on - strand, within GFF2958 at 386.861 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.990 kb on + strand, within GFF2958 at 386.990 kb on + strand, within GFF2958 at 386.990 kb on + strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.497 kb on + strand at 387.497 kb on + strand at 387.497 kb on + strand at 387.497 kb on + strand at 387.498 kb on - strand at 387.498 kb on - strand at 387.624 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.640 kb on - strand at 387.640 kb on - strand at 388.006 kb on + strand, within GFF2959 at 388.006 kb on + strand, within GFF2959 at 388.006 kb on + strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.120 kb on + strand, within GFF2959 at 388.120 kb on + strand, within GFF2959 at 388.120 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.241 kb on - strand, within GFF2959 at 388.676 kb on + strand, within GFF2960 at 388.677 kb on - strand, within GFF2960 at 388.677 kb on - strand, within GFF2960 at 388.677 kb on - strand, within GFF2960 at 388.677 kb on - strand, within GFF2960 at 388.749 kb on - strand, within GFF2960 at 388.749 kb on - strand, within GFF2960 at 388.907 kb on + strand, within GFF2960 at 388.908 kb on - strand, within GFF2960 at 388.908 kb on - strand, within GFF2960 at 388.908 kb on - strand, within GFF2960 at 388.908 kb on - strand, within GFF2960 at 389.021 kb on + strand at 389.021 kb on + strand at 389.022 kb on - strand at 389.022 kb on - strand at 389.022 kb on - strand at 389.022 kb on - strand at 389.022 kb on - strand at 389.033 kb on - strand at 389.033 kb on - strand at 389.069 kb on + strand at 389.069 kb on + strand at 389.069 kb on + strand at 389.069 kb on + strand at 389.069 kb on + strand at 389.070 kb on - strand at 389.070 kb on - strand at 389.070 kb on - strand at 389.070 kb on - strand at 389.075 kb on + strand at 389.076 kb on - strand at 389.083 kb on + strand at 389.083 kb on + strand at 389.083 kb on + strand at 389.083 kb on + strand at 389.083 kb on + strand at 389.084 kb on - strand at 389.084 kb on - strand at 389.084 kb on - strand at 389.084 kb on - strand at 389.084 kb on - strand at 389.084 kb on - strand at 389.154 kb on + strand at 389.154 kb on + strand at 389.154 kb on + strand at 389.154 kb on + strand at 389.154 kb on + strand at 389.155 kb on - strand at 389.155 kb on - strand at 389.155 kb on - strand at 389.155 kb on - strand at 389.155 kb on - strand at 389.155 kb on - strand at 389.155 kb on - strand at 389.155 kb on - strand at 389.271 kb on + strand at 389.271 kb on + strand at 389.271 kb on + strand at 389.271 kb on + strand at 389.272 kb on - strand at 389.272 kb on - strand at 389.272 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 386,721 - GFF2958 0.50 -0.1 386,861 + GFF2958 0.58 -1.3 386,909 + GFF2958 0.61 +0.2 386,909 + GFF2958 0.61 -0.6 386,909 + GFF2958 0.61 -0.4 386,909 + GFF2958 0.61 -1.1 386,910 - GFF2958 0.61 -1.6 386,910 - GFF2958 0.61 -1.8 386,910 - GFF2958 0.61 -0.2 386,910 - GFF2958 0.61 -0.5 386,910 - GFF2958 0.61 +0.5 386,990 + GFF2958 0.66 -0.2 386,990 + GFF2958 0.66 -0.6 386,990 + GFF2958 0.66 -0.6 386,991 - GFF2958 0.66 -0.7 386,991 - GFF2958 0.66 +0.2 386,991 - GFF2958 0.66 +0.6 386,991 - GFF2958 0.66 -0.6 386,991 - GFF2958 0.66 -1.5 386,991 - GFF2958 0.66 -0.8 386,991 - GFF2958 0.66 +0.4 386,991 - GFF2958 0.66 -0.5 387,144 - GFF2958 0.75 -0.2 387,144 - GFF2958 0.75 -0.8 387,144 - GFF2958 0.75 +0.1 387,144 - GFF2958 0.75 -0.3 387,497 + -0.8 387,497 + +0.1 387,497 + +0.4 387,497 + -0.5 387,498 - +0.3 387,498 - -0.4 387,624 + +0.5 387,639 + -0.1 387,639 + -0.4 387,639 + -0.3 387,639 + -0.6 387,639 + -0.3 387,640 - -0.8 387,640 - +0.1 388,006 + GFF2959 0.52 -0.6 388,006 + GFF2959 0.52 -0.8 388,006 + GFF2959 0.52 +0.5 388,007 - GFF2959 0.52 +0.3 388,007 - GFF2959 0.52 -1.5 388,007 - GFF2959 0.52 -3.4 388,007 - GFF2959 0.52 -0.4 388,120 + GFF2959 0.66 -0.4 388,120 + GFF2959 0.66 +0.5 388,120 + GFF2959 0.66 +0.7 388,222 + GFF2959 0.79 +0.5 388,222 + GFF2959 0.79 -0.1 388,222 + GFF2959 0.79 -0.6 388,222 + GFF2959 0.79 +1.3 388,222 + GFF2959 0.79 +0.7 388,222 + GFF2959 0.79 -1.5 388,240 + GFF2959 0.81 +2.3 388,240 + GFF2959 0.81 -0.8 388,240 + GFF2959 0.81 -0.7 388,240 + GFF2959 0.81 -0.5 388,241 - GFF2959 0.81 -2.2 388,676 + GFF2960 0.38 -0.1 388,677 - GFF2960 0.38 -0.0 388,677 - GFF2960 0.38 -0.4 388,677 - GFF2960 0.38 +0.5 388,677 - GFF2960 0.38 +0.2 388,749 - GFF2960 0.51 +2.2 388,749 - GFF2960 0.51 -0.5 388,907 + GFF2960 0.79 +0.1 388,908 - GFF2960 0.79 +0.1 388,908 - GFF2960 0.79 -0.9 388,908 - GFF2960 0.79 +0.2 388,908 - GFF2960 0.79 -0.6 389,021 + -2.8 389,021 + -1.8 389,022 - -0.9 389,022 - -0.5 389,022 - -0.2 389,022 - +1.1 389,022 - -0.8 389,033 - -0.0 389,033 - -1.0 389,069 + -0.2 389,069 + +1.5 389,069 + -0.1 389,069 + +0.9 389,069 + -0.5 389,070 - -0.1 389,070 - +1.1 389,070 - -1.2 389,070 - -0.8 389,075 + -1.5 389,076 - -0.6 389,083 + -0.3 389,083 + -0.5 389,083 + -0.6 389,083 + -0.5 389,083 + -1.0 389,084 - -0.5 389,084 - -1.0 389,084 - -0.1 389,084 - +0.1 389,084 - +0.7 389,084 - -1.2 389,154 + -0.3 389,154 + +0.9 389,154 + -0.4 389,154 + -0.5 389,154 + -0.7 389,155 - -0.1 389,155 - -0.4 389,155 - -0.5 389,155 - +0.4 389,155 - +0.1 389,155 - -0.6 389,155 - -0.5 389,155 - -0.0 389,271 + -0.5 389,271 + -0.5 389,271 + +0.6 389,271 + +0.1 389,272 - -0.9 389,272 - +0.1 389,272 - +0.2
Or see this region's nucleotide sequence