Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2914 and GFF2915 are separated by 56 nucleotides GFF2915 and GFF2916 are separated by 14 nucleotides GFF2916 and GFF2917 overlap by 11 nucleotides GFF2917 and GFF2918 are separated by 56 nucleotides
GFF2914 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 341,379 to 342,107
GFF2914
GFF2915 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 342,164 to 342,958
GFF2915
GFF2916 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 342,973 to 343,794
GFF2916
GFF2917 - Gluconolactonase (EC 3.1.1.17), at 343,784 to 344,674
GFF2917
GFF2918 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 344,731 to 345,723
GFF2918
Position (kb) 342
343
344 Strain fitness (log2 ratio) -1
0
1
2 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.010 kb on - strand, within GFF2914 at 342.105 kb on + strand at 342.106 kb on - strand at 342.141 kb on - strand at 342.669 kb on - strand, within GFF2915 at 342.908 kb on + strand at 342.908 kb on + strand at 342.909 kb on - strand at 342.917 kb on + strand at 342.917 kb on + strand at 342.918 kb on - strand at 342.918 kb on - strand at 343.273 kb on + strand, within GFF2916 at 343.273 kb on + strand, within GFF2916 at 343.273 kb on + strand, within GFF2916 at 343.274 kb on - strand, within GFF2916 at 343.342 kb on + strand, within GFF2916 at 343.342 kb on + strand, within GFF2916 at 343.393 kb on + strand, within GFF2916 at 343.393 kb on + strand, within GFF2916 at 343.397 kb on - strand, within GFF2916 at 343.745 kb on - strand at 343.745 kb on - strand at 344.180 kb on + strand, within GFF2917 at 344.180 kb on + strand, within GFF2917 at 344.180 kb on + strand, within GFF2917 at 344.181 kb on - strand, within GFF2917 at 344.181 kb on - strand, within GFF2917 at 344.181 kb on - strand, within GFF2917 at 344.187 kb on - strand, within GFF2917 at 344.187 kb on - strand, within GFF2917 at 344.276 kb on + strand, within GFF2917 at 344.277 kb on - strand, within GFF2917 at 344.277 kb on - strand, within GFF2917 at 344.741 kb on + strand at 344.742 kb on - strand at 344.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 342,009 + GFF2914 0.86 +0.8 342,009 + GFF2914 0.86 -0.0 342,009 + GFF2914 0.86 -0.2 342,009 + GFF2914 0.86 +0.5 342,009 + GFF2914 0.86 -0.1 342,010 - GFF2914 0.87 +1.2 342,105 + -0.4 342,106 - +0.4 342,141 - -0.6 342,669 - GFF2915 0.64 +2.1 342,908 + +0.1 342,908 + +1.0 342,909 - +0.0 342,917 + -1.3 342,917 + -0.8 342,918 - -0.3 342,918 - -0.2 343,273 + GFF2916 0.36 -0.4 343,273 + GFF2916 0.36 -1.1 343,273 + GFF2916 0.36 -1.3 343,274 - GFF2916 0.37 -0.6 343,342 + GFF2916 0.45 -0.2 343,342 + GFF2916 0.45 -1.3 343,393 + GFF2916 0.51 -0.5 343,393 + GFF2916 0.51 -0.1 343,397 - GFF2916 0.52 +1.9 343,745 - +0.2 343,745 - +0.1 344,180 + GFF2917 0.44 -0.5 344,180 + GFF2917 0.44 +0.1 344,180 + GFF2917 0.44 -0.8 344,181 - GFF2917 0.45 -1.2 344,181 - GFF2917 0.45 +0.9 344,181 - GFF2917 0.45 -0.7 344,187 - GFF2917 0.45 -0.3 344,187 - GFF2917 0.45 -0.6 344,276 + GFF2917 0.55 -0.4 344,277 - GFF2917 0.55 -0.6 344,277 - GFF2917 0.55 -0.6 344,741 + -0.7 344,742 - +0.8 344,742 - +1.5
Or see this region's nucleotide sequence