Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2790 and GFF2791 are separated by 116 nucleotides GFF2791 and GFF2792 are separated by 6 nucleotides GFF2792 and GFF2793 are separated by 109 nucleotides
GFF2790 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 219,707 to 220,891
GFF2790
GFF2791 - hypothetical protein, at 221,008 to 221,343
GFF2791
GFF2792 - Chaperone protein ClpB (ATP-dependent unfoldase), at 221,350 to 223,971
GFF2792
GFF2793 - Tricarboxylate transport protein TctC, at 224,081 to 225,073
GFF2793
Position (kb) 221
222
223
224 Strain fitness (log2 ratio) -2
-1
0
1
2 at 220.383 kb on + strand, within GFF2790 at 220.383 kb on + strand, within GFF2790 at 220.383 kb on + strand, within GFF2790 at 220.384 kb on - strand, within GFF2790 at 220.384 kb on - strand, within GFF2790 at 220.384 kb on - strand, within GFF2790 at 220.458 kb on + strand, within GFF2790 at 220.458 kb on + strand, within GFF2790 at 220.458 kb on + strand, within GFF2790 at 220.458 kb on + strand, within GFF2790 at 220.459 kb on - strand, within GFF2790 at 220.459 kb on - strand, within GFF2790 at 220.459 kb on - strand, within GFF2790 at 220.459 kb on - strand, within GFF2790 at 220.459 kb on - strand, within GFF2790 at 220.495 kb on - strand, within GFF2790 at 220.495 kb on - strand, within GFF2790 at 220.515 kb on + strand, within GFF2790 at 220.515 kb on + strand, within GFF2790 at 220.515 kb on + strand, within GFF2790 at 220.692 kb on + strand, within GFF2790 at 220.692 kb on + strand, within GFF2790 at 220.692 kb on + strand, within GFF2790 at 220.693 kb on - strand, within GFF2790 at 220.996 kb on + strand at 220.997 kb on - strand at 220.997 kb on - strand at 221.237 kb on + strand, within GFF2791 at 221.238 kb on - strand, within GFF2791 at 221.333 kb on - strand at 221.351 kb on + strand at 221.352 kb on - strand at 221.661 kb on + strand, within GFF2792 at 221.661 kb on + strand, within GFF2792 at 221.662 kb on - strand, within GFF2792 at 221.662 kb on - strand, within GFF2792 at 221.723 kb on + strand, within GFF2792 at 221.723 kb on + strand, within GFF2792 at 221.724 kb on - strand, within GFF2792 at 221.724 kb on - strand, within GFF2792 at 221.724 kb on - strand, within GFF2792 at 221.724 kb on - strand, within GFF2792 at 221.724 kb on - strand, within GFF2792 at 221.982 kb on - strand, within GFF2792 at 221.996 kb on + strand, within GFF2792 at 221.997 kb on - strand, within GFF2792 at 222.005 kb on + strand, within GFF2792 at 222.005 kb on + strand, within GFF2792 at 222.005 kb on + strand, within GFF2792 at 222.471 kb on - strand, within GFF2792 at 222.605 kb on + strand, within GFF2792 at 222.605 kb on + strand, within GFF2792 at 222.605 kb on + strand, within GFF2792 at 222.605 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.839 kb on + strand, within GFF2792 at 222.840 kb on - strand, within GFF2792 at 222.840 kb on - strand, within GFF2792 at 222.840 kb on - strand, within GFF2792 at 222.840 kb on - strand, within GFF2792 at 222.840 kb on - strand, within GFF2792 at 222.840 kb on - strand, within GFF2792 at 222.908 kb on + strand, within GFF2792 at 222.908 kb on + strand, within GFF2792 at 222.908 kb on + strand, within GFF2792 at 222.908 kb on + strand, within GFF2792 at 222.908 kb on + strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 222.909 kb on - strand, within GFF2792 at 223.013 kb on + strand, within GFF2792 at 223.013 kb on + strand, within GFF2792 at 223.013 kb on + strand, within GFF2792 at 223.013 kb on + strand, within GFF2792 at 223.013 kb on + strand, within GFF2792 at 223.013 kb on + strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.014 kb on - strand, within GFF2792 at 223.321 kb on + strand, within GFF2792 at 223.322 kb on - strand, within GFF2792 at 223.335 kb on + strand, within GFF2792 at 223.487 kb on + strand, within GFF2792 at 223.487 kb on + strand, within GFF2792 at 223.487 kb on + strand, within GFF2792 at 223.488 kb on - strand, within GFF2792 at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.995 kb on - strand at 223.995 kb on - strand at 224.389 kb on - strand, within GFF2793 at 224.391 kb on - strand, within GFF2793 at 224.522 kb on + strand, within GFF2793 at 224.522 kb on + strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.853 kb on - strand, within GFF2793 at 224.853 kb on - strand, within GFF2793 at 224.853 kb on - strand, within GFF2793 at 224.855 kb on + strand, within GFF2793 at 224.855 kb on + strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.904 kb on - strand, within GFF2793 at 224.904 kb on - strand, within GFF2793 at 224.904 kb on - strand, within GFF2793
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 220,383 + GFF2790 0.57 -0.8 220,383 + GFF2790 0.57 -0.4 220,383 + GFF2790 0.57 -1.3 220,384 - GFF2790 0.57 -0.2 220,384 - GFF2790 0.57 +0.1 220,384 - GFF2790 0.57 -1.3 220,458 + GFF2790 0.63 -1.4 220,458 + GFF2790 0.63 +0.2 220,458 + GFF2790 0.63 +0.5 220,458 + GFF2790 0.63 -0.8 220,459 - GFF2790 0.63 +0.9 220,459 - GFF2790 0.63 -0.4 220,459 - GFF2790 0.63 +0.8 220,459 - GFF2790 0.63 +0.3 220,459 - GFF2790 0.63 -0.7 220,495 - GFF2790 0.66 -0.7 220,495 - GFF2790 0.66 -0.0 220,515 + GFF2790 0.68 -0.1 220,515 + GFF2790 0.68 -0.4 220,515 + GFF2790 0.68 +0.2 220,692 + GFF2790 0.83 -0.1 220,692 + GFF2790 0.83 -0.4 220,692 + GFF2790 0.83 -1.5 220,693 - GFF2790 0.83 -0.4 220,996 + +0.3 220,997 - -0.3 220,997 - +0.5 221,237 + GFF2791 0.68 -0.6 221,238 - GFF2791 0.68 -0.4 221,333 - -1.6 221,351 + +0.3 221,352 - -0.4 221,661 + GFF2792 0.12 +0.2 221,661 + GFF2792 0.12 -1.0 221,662 - GFF2792 0.12 -0.6 221,662 - GFF2792 0.12 +0.0 221,723 + GFF2792 0.14 -0.5 221,723 + GFF2792 0.14 -0.1 221,724 - GFF2792 0.14 +0.5 221,724 - GFF2792 0.14 +2.0 221,724 - GFF2792 0.14 +0.4 221,724 - GFF2792 0.14 -0.2 221,724 - GFF2792 0.14 -0.6 221,982 - GFF2792 0.24 -0.0 221,996 + GFF2792 0.25 -0.7 221,997 - GFF2792 0.25 -0.1 222,005 + GFF2792 0.25 -0.4 222,005 + GFF2792 0.25 -0.8 222,005 + GFF2792 0.25 -0.3 222,471 - GFF2792 0.43 -0.6 222,605 + GFF2792 0.48 -1.1 222,605 + GFF2792 0.48 -0.3 222,605 + GFF2792 0.48 -0.0 222,605 + GFF2792 0.48 -0.4 222,839 + GFF2792 0.57 -0.5 222,839 + GFF2792 0.57 -0.1 222,839 + GFF2792 0.57 +0.4 222,839 + GFF2792 0.57 +1.0 222,839 + GFF2792 0.57 -1.1 222,839 + GFF2792 0.57 -1.5 222,839 + GFF2792 0.57 -0.4 222,839 + GFF2792 0.57 +1.5 222,839 + GFF2792 0.57 +0.7 222,839 + GFF2792 0.57 -1.2 222,839 + GFF2792 0.57 -1.9 222,840 - GFF2792 0.57 -0.9 222,840 - GFF2792 0.57 +0.7 222,840 - GFF2792 0.57 +0.2 222,840 - GFF2792 0.57 -1.5 222,840 - GFF2792 0.57 -0.3 222,840 - GFF2792 0.57 -1.9 222,908 + GFF2792 0.59 -0.4 222,908 + GFF2792 0.59 +0.2 222,908 + GFF2792 0.59 +0.2 222,908 + GFF2792 0.59 -0.5 222,908 + GFF2792 0.59 -0.1 222,909 - GFF2792 0.59 +0.8 222,909 - GFF2792 0.59 -0.6 222,909 - GFF2792 0.59 +0.7 222,909 - GFF2792 0.59 -0.1 222,909 - GFF2792 0.59 +0.5 222,909 - GFF2792 0.59 -0.5 222,909 - GFF2792 0.59 -0.1 222,909 - GFF2792 0.59 -0.8 222,909 - GFF2792 0.59 +0.7 223,013 + GFF2792 0.63 -0.1 223,013 + GFF2792 0.63 +0.5 223,013 + GFF2792 0.63 -0.9 223,013 + GFF2792 0.63 +0.7 223,013 + GFF2792 0.63 -0.5 223,013 + GFF2792 0.63 +0.0 223,014 - GFF2792 0.63 +1.2 223,014 - GFF2792 0.63 -0.9 223,014 - GFF2792 0.63 +0.1 223,014 - GFF2792 0.63 -0.7 223,014 - GFF2792 0.63 +0.2 223,014 - GFF2792 0.63 -1.1 223,014 - GFF2792 0.63 -1.3 223,014 - GFF2792 0.63 -0.2 223,321 + GFF2792 0.75 +0.7 223,322 - GFF2792 0.75 +0.5 223,335 + GFF2792 0.76 -1.9 223,487 + GFF2792 0.82 -0.2 223,487 + GFF2792 0.82 +0.0 223,487 + GFF2792 0.82 +1.1 223,488 - GFF2792 0.82 -1.1 223,889 + +0.8 223,889 + -0.0 223,889 + -0.5 223,889 + +0.6 223,889 + -1.1 223,889 + -0.0 223,889 + +0.5 223,890 - -1.1 223,890 - +0.2 223,890 - +0.1 223,890 - +0.1 223,890 - +0.5 223,890 - +2.5 223,995 - +0.4 223,995 - +0.2 224,389 - GFF2793 0.31 -0.1 224,391 - GFF2793 0.31 +0.1 224,522 + GFF2793 0.44 +0.3 224,522 + GFF2793 0.44 +1.5 224,523 - GFF2793 0.45 -0.2 224,523 - GFF2793 0.45 -1.5 224,523 - GFF2793 0.45 +0.8 224,523 - GFF2793 0.45 +0.6 224,523 - GFF2793 0.45 -0.8 224,624 + GFF2793 0.55 +0.7 224,624 + GFF2793 0.55 +0.3 224,624 + GFF2793 0.55 -0.5 224,624 + GFF2793 0.55 -0.8 224,624 + GFF2793 0.55 +0.3 224,624 + GFF2793 0.55 -0.5 224,624 + GFF2793 0.55 -0.5 224,624 + GFF2793 0.55 +0.2 224,624 + GFF2793 0.55 -0.5 224,624 + GFF2793 0.55 -0.4 224,624 + GFF2793 0.55 -0.1 224,625 - GFF2793 0.55 +0.1 224,625 - GFF2793 0.55 -1.1 224,625 - GFF2793 0.55 -0.0 224,625 - GFF2793 0.55 -0.2 224,625 - GFF2793 0.55 -1.1 224,852 + GFF2793 0.78 -0.1 224,852 + GFF2793 0.78 +0.1 224,852 + GFF2793 0.78 -0.3 224,852 + GFF2793 0.78 -0.3 224,853 - GFF2793 0.78 -0.9 224,853 - GFF2793 0.78 -0.1 224,853 - GFF2793 0.78 +0.3 224,855 + GFF2793 0.78 -0.9 224,855 + GFF2793 0.78 -0.1 224,856 - GFF2793 0.78 -2.4 224,856 - GFF2793 0.78 +0.7 224,856 - GFF2793 0.78 -0.3 224,856 - GFF2793 0.78 -0.7 224,856 - GFF2793 0.78 -0.6 224,856 - GFF2793 0.78 +0.2 224,856 - GFF2793 0.78 -0.3 224,903 + GFF2793 0.83 +0.5 224,903 + GFF2793 0.83 -0.9 224,903 + GFF2793 0.83 -0.1 224,903 + GFF2793 0.83 +0.4 224,904 - GFF2793 0.83 -1.0 224,904 - GFF2793 0.83 -0.3 224,904 - GFF2793 0.83 -0.2
Or see this region's nucleotide sequence