Strain Fitness in Variovorax sp. SCN45 around GFF2596

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
1,048	+				+0.2
1,048	+				-1.0
1,048	+				-0.1
1,075	+				-0.8
1,075	+				-2.1
1,088	-				-0.2
1,088	-				-0.5
1,139	-				+0.7
1,139	-				+0.5
1,144	+				+0.5
1,145	-				-0.1
1,411	+		GFF2595	0.32	+1.1
1,411	+		GFF2595	0.32	-1.1
1,411	+		GFF2595	0.32	+0.6
1,411	+		GFF2595	0.32	-0.6
1,411	+		GFF2595	0.32	-0.6
1,411	+		GFF2595	0.32	-0.9
1,411	+		GFF2595	0.32	-0.8
1,412	-		GFF2595	0.32	-0.5
1,412	-		GFF2595	0.32	+0.1
1,412	-		GFF2595	0.32	-1.1
1,412	-		GFF2595	0.32	-0.9
1,588	+		GFF2595	0.55	+0.2
1,588	+		GFF2595	0.55	-0.2
1,805	-		GFF2595	0.83	-0.5
2,043	+				+0.5
2,043	+				-0.5
2,043	+				-0.1
2,043	+				-0.5
2,043	+				-0.6
2,044	-				-0.4
2,044	-				+0.2
2,049	+				-0.1
2,049	+				+2.5
2,049	+				-0.9
2,208	+		GFF2596	0.23	-0.2
2,208	+		GFF2596	0.23	+0.7
2,208	+		GFF2596	0.23	+0.5
2,208	+		GFF2596	0.23	-0.1
2,208	+		GFF2596	0.23	-0.9
2,208	+		GFF2596	0.23	-0.2
2,209	-		GFF2596	0.23	-0.1
2,209	-		GFF2596	0.23	-1.1
2,209	-		GFF2596	0.23	-0.4
2,209	-		GFF2596	0.23	-0.9
2,209	-		GFF2596	0.23	+0.6
2,209	-		GFF2596	0.23	-0.1
2,209	-		GFF2596	0.23	+0.9
2,332	-		GFF2596	0.33	+2.5
2,332	-		GFF2596	0.33	-0.2
2,373	+		GFF2596	0.36	+0.2
2,373	+		GFF2596	0.36	+0.7
2,373	+		GFF2596	0.36	+0.5
2,373	+		GFF2596	0.36	+1.3
2,373	+		GFF2596	0.36	-1.1
2,374	-		GFF2596	0.36	-0.2
2,374	-		GFF2596	0.36	+0.3
2,430	+		GFF2596	0.41	+0.0
2,439	+		GFF2596	0.42	+1.1
2,439	+		GFF2596	0.42	+0.5
2,439	+		GFF2596	0.42	+0.4
2,439	+		GFF2596	0.42	-0.2
2,440	-		GFF2596	0.42	-3.3
2,440	-		GFF2596	0.42	-1.2
2,440	-		GFF2596	0.42	-1.1
2,440	-		GFF2596	0.42	+0.1
2,440	-		GFF2596	0.42	-0.5
2,440	-		GFF2596	0.42	+0.0
2,440	-		GFF2596	0.42	+1.2
2,440	-		GFF2596	0.42	-0.4
2,481	+		GFF2596	0.45	+0.1
2,481	+		GFF2596	0.45	+0.6
2,482	-		GFF2596	0.45	-0.5
2,482	-		GFF2596	0.45	-1.5
2,604	+		GFF2596	0.55	-0.8
2,604	+		GFF2596	0.55	+0.6
2,605	-		GFF2596	0.55	-0.1
2,634	+		GFF2596	0.58	+1.0
2,634	+		GFF2596	0.58	-0.1
2,635	-		GFF2596	0.58	+0.2
2,635	-		GFF2596	0.58	+0.9
2,635	-		GFF2596	0.58	-0.3
2,635	-		GFF2596	0.58	-1.4
2,898	+		GFF2596	0.79	-0.7
2,898	+		GFF2596	0.79	+0.5
2,898	+		GFF2596	0.79	+0.1
2,898	+		GFF2596	0.79	-1.6
2,898	+		GFF2596	0.79	-0.8
2,898	+		GFF2596	0.79	-0.4
2,898	+		GFF2596	0.79	+0.1
2,899	-		GFF2596	0.79	-0.6
2,899	-		GFF2596	0.79	+0.9
2,899	-		GFF2596	0.79	-0.3
2,899	-		GFF2596	0.79	-1.0
2,899	-		GFF2596	0.79	+0.7
2,899	-		GFF2596	0.79	-0.2
2,899	-		GFF2596	0.79	+0.5
2,899	-		GFF2596	0.79	-0.3
2,899	-		GFF2596	0.79	+1.5
2,899	-		GFF2596	0.79	-0.7
2,899	-		GFF2596	0.79	-0.5
3,054	+				+0.7
3,054	+				+0.2
3,054	+				+0.2
3,054	+				-0.3
3,054	+				+1.1
3,055	-				-0.5
3,055	-				-0.5
3,055	-				-0.1
3,055	-				-0.1
3,055	-				-0.3
3,055	-				-0.0
3,055	-				-0.0
3,248	+		GFF2597	0.23	-0.5
3,248	+		GFF2597	0.23	+0.5
3,248	+		GFF2597	0.23	+2.0
3,249	-		GFF2597	0.23	-0.1
3,386	+		GFF2597	0.55	-0.3
3,386	+		GFF2597	0.55	-0.4
3,387	-		GFF2597	0.55	+0.6
3,387	-		GFF2597	0.55	-0.2
3,551	+				+0.1
3,551	+				+0.5
3,551	+				-0.0
3,551	+				-1.4
3,552	-				-1.1
3,552	-				+0.8
3,552	-				-0.2
4,022	-		GFF2598	0.36	-2.0
4,022	-		GFF2598	0.36	-0.2
4,022	-		GFF2598	0.36	+0.1
4,022	-		GFF2598	0.36	-0.2
4,022	-		GFF2598	0.36	-0.2