Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2543 and GFF2544 are separated by 131 nucleotides GFF2544 and GFF2545 overlap by 4 nucleotides GFF2545 and GFF2546 are separated by 13 nucleotides
GFF2543 - Uncharacterized membrane protein Bcep18194_A6058, at 606,046 to 607,077
GFF2543
GFF2544 - Ubiquinone biosynthesis protein UbiJ, at 607,209 to 607,778
GFF2544
GFF2545 - Ubiquinone biosynthesis regulatory protein kinase UbiB, at 607,775 to 609,340
GFF2545
GFF2546 - sodium-solute symporter, putative, at 609,354 to 610,805
GFF2546
Position (kb)
607
608
609
610 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.934 kb on + strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 606.935 kb on - strand, within GFF2543 at 607.075 kb on + strand at 607.075 kb on + strand at 607.075 kb on + strand at 607.075 kb on + strand at 607.075 kb on + strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.076 kb on - strand at 607.080 kb on + strand at 607.080 kb on + strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.081 kb on - strand at 607.188 kb on - strand at 607.199 kb on - strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.208 kb on + strand at 607.209 kb on - strand at 607.209 kb on - strand at 607.209 kb on - strand at 607.787 kb on + strand at 609.291 kb on + strand at 609.338 kb on + strand at 609.338 kb on + strand at 609.339 kb on - strand at 609.339 kb on - strand at 609.339 kb on - strand at 609.378 kb on + strand at 609.378 kb on + strand at 609.378 kb on + strand at 609.378 kb on + strand at 609.378 kb on + strand at 609.378 kb on + strand at 609.379 kb on - strand at 609.379 kb on - strand at 609.379 kb on - strand at 609.379 kb on - strand at 609.379 kb on - strand at 609.408 kb on + strand at 609.408 kb on + strand at 609.408 kb on + strand at 609.408 kb on + strand at 609.409 kb on - strand at 609.409 kb on - strand at 609.409 kb on - strand at 609.409 kb on - strand at 609.409 kb on - strand at 609.409 kb on - strand at 609.633 kb on + strand, within GFF2546 at 609.634 kb on - strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.663 kb on + strand, within GFF2546 at 609.664 kb on - strand, within GFF2546 at 609.664 kb on - strand, within GFF2546 at 609.664 kb on - strand, within GFF2546 at 609.664 kb on - strand, within GFF2546 at 609.664 kb on - strand, within GFF2546 at 609.666 kb on + strand, within GFF2546 at 609.666 kb on + strand, within GFF2546 at 609.666 kb on + strand, within GFF2546 at 609.679 kb on - strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.726 kb on + strand, within GFF2546 at 609.727 kb on - strand, within GFF2546 at 609.727 kb on - strand, within GFF2546 at 609.843 kb on + strand, within GFF2546 at 609.843 kb on + strand, within GFF2546 at 609.843 kb on + strand, within GFF2546 at 609.843 kb on + strand, within GFF2546 at 609.843 kb on + strand, within GFF2546 at 609.843 kb on + strand, within GFF2546 at 609.843 kb on + strand, within GFF2546 at 609.844 kb on - strand, within GFF2546 at 609.844 kb on - strand, within GFF2546 at 609.844 kb on - strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.161 kb on + strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546 at 610.162 kb on - strand, within GFF2546
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 606,934 + GFF2543 0.86 -1.9 606,934 + GFF2543 0.86 -2.6 606,934 + GFF2543 0.86 -2.7 606,934 + GFF2543 0.86 -0.9 606,934 + GFF2543 0.86 -2.3 606,934 + GFF2543 0.86 -1.4 606,934 + GFF2543 0.86 -0.3 606,934 + GFF2543 0.86 -0.7 606,934 + GFF2543 0.86 -2.2 606,934 + GFF2543 0.86 -0.1 606,935 - GFF2543 0.86 -1.6 606,935 - GFF2543 0.86 -0.7 606,935 - GFF2543 0.86 -0.5 606,935 - GFF2543 0.86 -1.4 606,935 - GFF2543 0.86 -0.8 606,935 - GFF2543 0.86 +0.1 606,935 - GFF2543 0.86 -0.9 606,935 - GFF2543 0.86 -0.5 606,935 - GFF2543 0.86 +0.2 606,935 - GFF2543 0.86 -1.0 606,935 - GFF2543 0.86 -0.9 606,935 - GFF2543 0.86 -0.9 607,075 + -1.6 607,075 + -0.4 607,075 + -0.3 607,075 + -0.3 607,075 + -0.3 607,076 - -1.8 607,076 - -0.0 607,076 - -0.1 607,076 - +0.3 607,076 - -1.2 607,076 - +0.1 607,076 - +0.3 607,076 - -0.1 607,076 - -0.5 607,076 - -1.1 607,080 + -0.5 607,080 + +1.7 607,081 - +0.4 607,081 - +1.1 607,081 - -0.4 607,081 - -0.0 607,081 - -0.5 607,081 - -0.3 607,081 - -0.2 607,081 - +0.2 607,081 - -0.6 607,188 - +0.7 607,199 - +1.1 607,208 + -1.2 607,208 + +0.1 607,208 + -1.2 607,208 + -0.4 607,208 + -1.2 607,208 + -0.6 607,208 + -0.7 607,208 + +0.1 607,208 + -0.2 607,208 + -1.3 607,209 - +2.2 607,209 - -2.1 607,209 - -0.1 607,787 + +0.9 609,291 + +0.3 609,338 + -0.2 609,338 + -1.1 609,339 - -0.2 609,339 - -0.4 609,339 - -0.2 609,378 + -0.9 609,378 + -0.7 609,378 + -0.0 609,378 + -1.4 609,378 + -1.4 609,378 + +0.4 609,379 - -1.9 609,379 - -0.3 609,379 - -0.7 609,379 - +0.3 609,379 - -0.8 609,408 + -0.4 609,408 + +0.2 609,408 + +0.7 609,408 + +0.7 609,409 - -0.8 609,409 - -0.6 609,409 - -0.4 609,409 - +0.1 609,409 - -1.1 609,409 - -0.5 609,633 + GFF2546 0.19 -0.0 609,634 - GFF2546 0.19 +0.2 609,663 + GFF2546 0.21 +0.7 609,663 + GFF2546 0.21 -0.6 609,663 + GFF2546 0.21 -0.7 609,663 + GFF2546 0.21 +0.6 609,663 + GFF2546 0.21 -1.7 609,663 + GFF2546 0.21 +1.0 609,663 + GFF2546 0.21 -0.5 609,663 + GFF2546 0.21 -0.0 609,664 - GFF2546 0.21 -0.1 609,664 - GFF2546 0.21 +0.7 609,664 - GFF2546 0.21 -0.4 609,664 - GFF2546 0.21 +0.2 609,664 - GFF2546 0.21 -0.5 609,666 + GFF2546 0.21 +0.1 609,666 + GFF2546 0.21 -0.1 609,666 + GFF2546 0.21 -1.1 609,679 - GFF2546 0.22 -0.4 609,726 + GFF2546 0.26 -0.7 609,726 + GFF2546 0.26 +2.3 609,726 + GFF2546 0.26 +0.1 609,726 + GFF2546 0.26 -0.3 609,726 + GFF2546 0.26 +0.7 609,726 + GFF2546 0.26 +0.3 609,726 + GFF2546 0.26 -0.5 609,726 + GFF2546 0.26 +0.5 609,727 - GFF2546 0.26 -0.2 609,727 - GFF2546 0.26 -0.5 609,843 + GFF2546 0.34 -0.1 609,843 + GFF2546 0.34 -1.8 609,843 + GFF2546 0.34 -0.7 609,843 + GFF2546 0.34 +0.6 609,843 + GFF2546 0.34 -0.3 609,843 + GFF2546 0.34 -0.3 609,843 + GFF2546 0.34 +0.1 609,844 - GFF2546 0.34 -0.1 609,844 - GFF2546 0.34 -0.1 609,844 - GFF2546 0.34 +0.3 610,161 + GFF2546 0.56 -0.2 610,161 + GFF2546 0.56 -0.3 610,161 + GFF2546 0.56 -0.1 610,161 + GFF2546 0.56 -0.1 610,161 + GFF2546 0.56 -1.0 610,161 + GFF2546 0.56 -0.4 610,161 + GFF2546 0.56 -0.2 610,161 + GFF2546 0.56 +0.7 610,161 + GFF2546 0.56 -0.1 610,161 + GFF2546 0.56 -0.9 610,161 + GFF2546 0.56 -0.3 610,161 + GFF2546 0.56 -0.6 610,161 + GFF2546 0.56 -0.7 610,161 + GFF2546 0.56 +0.1 610,161 + GFF2546 0.56 -0.2 610,161 + GFF2546 0.56 +0.0 610,161 + GFF2546 0.56 -0.7 610,161 + GFF2546 0.56 +0.7 610,161 + GFF2546 0.56 -0.1 610,161 + GFF2546 0.56 +1.0 610,161 + GFF2546 0.56 -1.0 610,161 + GFF2546 0.56 +0.1 610,162 - GFF2546 0.56 -0.5 610,162 - GFF2546 0.56 +0.7 610,162 - GFF2546 0.56 -2.6 610,162 - GFF2546 0.56 -1.1 610,162 - GFF2546 0.56 +0.1 610,162 - GFF2546 0.56 -0.5 610,162 - GFF2546 0.56 -0.1 610,162 - GFF2546 0.56 -0.9 610,162 - GFF2546 0.56 -1.1 610,162 - GFF2546 0.56 +0.7 610,162 - GFF2546 0.56 +0.1 610,162 - GFF2546 0.56 +0.8 610,162 - GFF2546 0.56 +0.7 610,162 - GFF2546 0.56 -0.8 610,162 - GFF2546 0.56 -0.8 610,162 - GFF2546 0.56 +0.5 610,162 - GFF2546 0.56 -0.5 610,162 - GFF2546 0.56 +0.7 610,162 - GFF2546 0.56 -0.5 610,162 - GFF2546 0.56 +0.1 610,162 - GFF2546 0.56 -0.9
Or see this region's nucleotide sequence