Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF244 and GFF245 overlap by 4 nucleotides GFF245 and GFF246 are separated by 131 nucleotides GFF246 and GFF247 are separated by 72 nucleotides
GFF244 - Cys regulon transcriptional activator CysB, at 271,858 to 272,799
GFF244
GFF245 - Aspartate aminotransferase (EC 2.6.1.1), at 272,796 to 273,968
GFF245
GFF246 - Transmembrane protein Msl3831, at 274,100 to 274,258
GFF246
GFF247 - ATP-dependent helicase HrpA, at 274,331 to 278,116
GFF247
Position (kb) 272
273
274 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 271.842 kb on + strand at 271.938 kb on + strand at 271.938 kb on + strand at 271.938 kb on + strand at 271.938 kb on + strand at 271.938 kb on + strand at 271.939 kb on - strand at 271.939 kb on - strand at 271.939 kb on - strand at 271.939 kb on - strand at 271.939 kb on - strand at 271.939 kb on - strand at 271.939 kb on - strand at 272.173 kb on + strand, within GFF244 at 272.173 kb on + strand, within GFF244 at 272.174 kb on - strand, within GFF244 at 272.347 kb on + strand, within GFF244 at 272.348 kb on - strand, within GFF244 at 272.348 kb on - strand, within GFF244 at 272.446 kb on + strand, within GFF244 at 272.446 kb on + strand, within GFF244 at 272.447 kb on - strand, within GFF244 at 272.447 kb on - strand, within GFF244 at 272.447 kb on - strand, within GFF244 at 272.554 kb on + strand, within GFF244 at 272.554 kb on + strand, within GFF244 at 272.554 kb on + strand, within GFF244 at 272.554 kb on + strand, within GFF244 at 272.554 kb on + strand, within GFF244 at 272.555 kb on - strand, within GFF244 at 272.555 kb on - strand, within GFF244 at 272.555 kb on - strand, within GFF244 at 272.662 kb on - strand, within GFF244 at 272.692 kb on + strand, within GFF244 at 272.693 kb on - strand, within GFF244 at 272.788 kb on + strand at 272.788 kb on + strand at 272.788 kb on + strand at 272.789 kb on - strand at 272.789 kb on - strand at 272.789 kb on - strand at 272.789 kb on - strand at 272.789 kb on - strand at 272.789 kb on - strand at 272.795 kb on + strand at 272.795 kb on + strand at 272.795 kb on + strand at 272.795 kb on + strand at 272.795 kb on + strand at 272.795 kb on + strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 272.796 kb on - strand at 273.057 kb on + strand, within GFF245 at 273.057 kb on + strand, within GFF245 at 273.058 kb on - strand, within GFF245 at 273.058 kb on - strand, within GFF245 at 273.058 kb on - strand, within GFF245 at 273.069 kb on + strand, within GFF245 at 273.069 kb on + strand, within GFF245 at 273.070 kb on - strand, within GFF245 at 273.180 kb on + strand, within GFF245 at 273.181 kb on - strand, within GFF245 at 273.181 kb on - strand, within GFF245 at 273.181 kb on - strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.189 kb on + strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.190 kb on - strand, within GFF245 at 273.420 kb on + strand, within GFF245 at 273.420 kb on + strand, within GFF245 at 273.421 kb on - strand, within GFF245 at 273.421 kb on - strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.645 kb on + strand, within GFF245 at 273.645 kb on + strand, within GFF245 at 273.645 kb on + strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.666 kb on + strand, within GFF245 at 273.687 kb on + strand, within GFF245 at 273.688 kb on - strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.873 kb on + strand at 273.873 kb on + strand at 273.873 kb on + strand at 273.874 kb on - strand at 273.874 kb on - strand at 273.874 kb on - strand at 273.874 kb on - strand at 274.000 kb on + strand at 274.140 kb on + strand, within GFF246 at 274.140 kb on + strand, within GFF246 at 274.141 kb on - strand, within GFF246 at 274.141 kb on - strand, within GFF246 at 274.147 kb on - strand, within GFF246 at 274.169 kb on + strand, within GFF246 at 274.169 kb on + strand, within GFF246 at 274.170 kb on - strand, within GFF246 at 274.170 kb on - strand, within GFF246 at 274.170 kb on - strand, within GFF246 at 274.171 kb on + strand, within GFF246 at 274.172 kb on - strand, within GFF246 at 274.187 kb on + strand, within GFF246 at 274.187 kb on + strand, within GFF246 at 274.187 kb on + strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.497 kb on + strand at 274.498 kb on - strand at 274.735 kb on - strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 271,842 + -2.3 271,938 + -2.9 271,938 + -2.1 271,938 + -0.1 271,938 + -1.4 271,938 + -2.9 271,939 - -2.9 271,939 - -1.0 271,939 - -0.8 271,939 - -3.4 271,939 - -2.7 271,939 - -2.4 271,939 - -1.4 272,173 + GFF244 0.33 -0.8 272,173 + GFF244 0.33 -0.4 272,174 - GFF244 0.34 -2.1 272,347 + GFF244 0.52 -1.6 272,348 - GFF244 0.52 -1.4 272,348 - GFF244 0.52 -0.8 272,446 + GFF244 0.62 -2.8 272,446 + GFF244 0.62 -3.3 272,447 - GFF244 0.63 -4.0 272,447 - GFF244 0.63 -2.8 272,447 - GFF244 0.63 -3.0 272,554 + GFF244 0.74 -1.8 272,554 + GFF244 0.74 +0.2 272,554 + GFF244 0.74 -2.7 272,554 + GFF244 0.74 -1.4 272,554 + GFF244 0.74 -0.0 272,555 - GFF244 0.74 -2.5 272,555 - GFF244 0.74 -2.5 272,555 - GFF244 0.74 -1.1 272,662 - GFF244 0.85 -0.7 272,692 + GFF244 0.89 -2.1 272,693 - GFF244 0.89 -1.1 272,788 + +1.0 272,788 + -0.5 272,788 + -0.0 272,789 - +0.5 272,789 - -0.6 272,789 - +0.4 272,789 - -0.9 272,789 - +0.6 272,789 - -0.8 272,795 + -0.7 272,795 + -0.8 272,795 + +0.1 272,795 + -0.2 272,795 + +3.2 272,795 + -0.3 272,796 - +0.6 272,796 - +0.6 272,796 - +1.2 272,796 - -1.4 272,796 - -1.8 272,796 - -4.7 272,796 - -1.1 272,796 - -2.3 272,796 - -2.1 272,796 - -2.5 272,796 - -1.4 272,796 - +1.2 272,796 - -0.3 272,796 - -1.4 273,057 + GFF245 0.22 -1.8 273,057 + GFF245 0.22 -2.0 273,058 - GFF245 0.22 -0.8 273,058 - GFF245 0.22 -0.2 273,058 - GFF245 0.22 +1.2 273,069 + GFF245 0.23 +0.0 273,069 + GFF245 0.23 -1.0 273,070 - GFF245 0.23 -1.8 273,180 + GFF245 0.33 -0.4 273,181 - GFF245 0.33 -0.2 273,181 - GFF245 0.33 -0.4 273,181 - GFF245 0.33 -0.5 273,189 + GFF245 0.34 -1.6 273,189 + GFF245 0.34 +1.7 273,189 + GFF245 0.34 -0.1 273,189 + GFF245 0.34 -1.6 273,189 + GFF245 0.34 +0.9 273,189 + GFF245 0.34 -0.4 273,189 + GFF245 0.34 -0.2 273,189 + GFF245 0.34 +0.2 273,189 + GFF245 0.34 -1.1 273,189 + GFF245 0.34 +0.3 273,189 + GFF245 0.34 -0.9 273,189 + GFF245 0.34 -0.7 273,190 - GFF245 0.34 -0.4 273,190 - GFF245 0.34 +0.4 273,190 - GFF245 0.34 -1.0 273,190 - GFF245 0.34 -0.1 273,190 - GFF245 0.34 -1.4 273,190 - GFF245 0.34 -0.9 273,190 - GFF245 0.34 -2.3 273,190 - GFF245 0.34 -1.1 273,420 + GFF245 0.53 -1.0 273,420 + GFF245 0.53 +0.2 273,421 - GFF245 0.53 -0.0 273,421 - GFF245 0.53 +0.1 273,519 + GFF245 0.62 -0.2 273,519 + GFF245 0.62 -0.1 273,519 + GFF245 0.62 +1.5 273,519 + GFF245 0.62 +0.4 273,519 + GFF245 0.62 -0.4 273,520 - GFF245 0.62 -0.6 273,520 - GFF245 0.62 -0.0 273,520 - GFF245 0.62 -3.3 273,520 - GFF245 0.62 -1.9 273,520 - GFF245 0.62 -0.5 273,645 + GFF245 0.72 -0.8 273,645 + GFF245 0.72 -0.3 273,645 + GFF245 0.72 +0.2 273,646 - GFF245 0.72 -0.1 273,646 - GFF245 0.72 -0.4 273,646 - GFF245 0.72 -0.6 273,646 - GFF245 0.72 -0.1 273,666 + GFF245 0.74 +0.8 273,687 + GFF245 0.76 +1.2 273,688 - GFF245 0.76 -1.1 273,765 + GFF245 0.83 +0.6 273,765 + GFF245 0.83 -0.6 273,765 + GFF245 0.83 -0.0 273,765 + GFF245 0.83 +0.2 273,766 - GFF245 0.83 -0.3 273,766 - GFF245 0.83 +0.2 273,766 - GFF245 0.83 -0.3 273,766 - GFF245 0.83 -0.8 273,766 - GFF245 0.83 +0.8 273,873 + -0.4 273,873 + -1.4 273,873 + -0.5 273,874 - -1.8 273,874 - -0.3 273,874 - -0.1 273,874 - -0.2 274,000 + +0.2 274,140 + GFF246 0.25 -0.3 274,140 + GFF246 0.25 -0.1 274,141 - GFF246 0.26 -1.0 274,141 - GFF246 0.26 -0.8 274,147 - GFF246 0.30 -0.6 274,169 + GFF246 0.43 -0.8 274,169 + GFF246 0.43 +1.9 274,170 - GFF246 0.44 -2.6 274,170 - GFF246 0.44 -0.1 274,170 - GFF246 0.44 -0.1 274,171 + GFF246 0.45 -0.0 274,172 - GFF246 0.45 +1.2 274,187 + GFF246 0.55 -1.5 274,187 + GFF246 0.55 -1.4 274,187 + GFF246 0.55 -1.1 274,188 - GFF246 0.55 -0.6 274,188 - GFF246 0.55 +0.3 274,188 - GFF246 0.55 -0.1 274,188 - GFF246 0.55 +1.2 274,188 - GFF246 0.55 -1.2 274,254 + -0.4 274,254 + +0.1 274,254 + +0.2 274,254 + +0.2 274,254 + +0.2 274,254 + -0.8 274,254 + +0.4 274,254 + -1.5 274,254 + +0.4 274,254 + -1.4 274,254 + -0.0 274,255 - -0.4 274,255 - +1.1 274,255 - -0.6 274,255 - -0.3 274,255 - -1.7 274,255 - -0.3 274,255 - -0.5 274,255 - -0.5 274,255 - +0.6 274,256 + -0.0 274,256 + -0.4 274,256 + -0.7 274,256 + +0.8 274,256 + -0.2 274,256 + -0.6 274,256 + -0.8 274,256 + +0.4 274,256 + -0.2 274,256 + -0.2 274,256 + -0.0 274,257 - -0.4 274,257 - +0.8 274,257 - -0.6 274,257 - +0.3 274,257 - -0.2 274,257 - +0.1 274,257 - -0.2 274,257 - -0.0 274,257 - +0.0 274,257 - -0.5 274,497 + -0.6 274,498 - +0.0 274,735 - GFF247 0.11 +1.2 274,923 + GFF247 0.16 +0.5 274,923 + GFF247 0.16 -1.0 274,923 + GFF247 0.16 -0.4 274,923 + GFF247 0.16 -0.4 274,924 - GFF247 0.16 +0.1 274,924 - GFF247 0.16 +1.4 274,924 - GFF247 0.16 +0.4 274,924 - GFF247 0.16 -0.5 274,924 - GFF247 0.16 -0.3 274,924 - GFF247 0.16 -0.3
Or see this region's nucleotide sequence