Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF234 and GFF235 are separated by 311 nucleotides GFF235 and GFF236 are separated by 100 nucleotides GFF236 and GFF237 are separated by 72 nucleotides
GFF234 - Protein QmcA (possibly involved in integral membrane quality control), at 263,309 to 264,238
GFF234
GFF235 - hypothetical protein, at 264,550 to 264,906
GFF235
GFF236 - Two-component transcriptional response regulator receiver domain, at 265,007 to 265,396
GFF236
GFF237 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 265,469 to 266,686
GFF237
Position (kb) 265
266 Strain fitness (log2 ratio) -2
-1
0
1
2 at 264.113 kb on + strand, within GFF234 at 264.114 kb on - strand, within GFF234 at 264.114 kb on - strand, within GFF234 at 264.248 kb on + strand at 264.248 kb on + strand at 264.248 kb on + strand at 264.248 kb on + strand at 264.248 kb on + strand at 264.248 kb on + strand at 264.249 kb on - strand at 264.249 kb on - strand at 264.371 kb on - strand at 264.507 kb on + strand at 264.507 kb on + strand at 264.507 kb on + strand at 264.507 kb on + strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.523 kb on + strand at 264.939 kb on - strand at 264.950 kb on + strand at 264.950 kb on + strand at 264.951 kb on - strand at 264.975 kb on + strand at 264.975 kb on + strand at 264.975 kb on + strand at 264.975 kb on + strand at 264.976 kb on - strand at 264.976 kb on - strand at 264.976 kb on - strand at 264.976 kb on - strand at 264.976 kb on - strand at 264.980 kb on + strand at 264.980 kb on + strand at 264.980 kb on + strand at 264.981 kb on - strand at 264.981 kb on - strand at 265.265 kb on + strand, within GFF236 at 265.265 kb on + strand, within GFF236 at 265.265 kb on + strand, within GFF236 at 265.265 kb on + strand, within GFF236 at 265.265 kb on + strand, within GFF236 at 265.265 kb on + strand, within GFF236 at 265.265 kb on + strand, within GFF236 at 265.265 kb on + strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.266 kb on - strand, within GFF236 at 265.318 kb on - strand, within GFF236 at 265.355 kb on + strand, within GFF236 at 265.355 kb on + strand, within GFF236 at 265.355 kb on + strand, within GFF236 at 265.355 kb on + strand, within GFF236 at 265.355 kb on + strand, within GFF236 at 265.355 kb on + strand, within GFF236 at 265.356 kb on - strand, within GFF236 at 265.356 kb on - strand, within GFF236 at 265.356 kb on - strand, within GFF236 at 265.356 kb on - strand, within GFF236 at 265.356 kb on - strand, within GFF236 at 265.356 kb on - strand, within GFF236 at 265.394 kb on + strand at 265.394 kb on + strand at 265.394 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.509 kb on + strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.510 kb on - strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.515 kb on + strand at 265.516 kb on - strand at 265.516 kb on - strand at 265.516 kb on - strand at 265.516 kb on - strand at 265.516 kb on - strand at 265.516 kb on - strand at 265.516 kb on - strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.584 kb on + strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.585 kb on - strand at 265.611 kb on + strand, within GFF237 at 265.675 kb on - strand, within GFF237 at 265.675 kb on - strand, within GFF237 at 265.675 kb on - strand, within GFF237 at 265.675 kb on - strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.680 kb on + strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.681 kb on - strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.689 kb on + strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 265.690 kb on - strand, within GFF237 at 266.080 kb on - strand, within GFF237 at 266.080 kb on - strand, within GFF237 at 266.086 kb on + strand, within GFF237 at 266.086 kb on + strand, within GFF237 at 266.086 kb on + strand, within GFF237 at 266.087 kb on - strand, within GFF237 at 266.136 kb on + strand, within GFF237 at 266.136 kb on + strand, within GFF237 at 266.136 kb on + strand, within GFF237 at 266.136 kb on + strand, within GFF237 at 266.136 kb on + strand, within GFF237 at 266.137 kb on - strand, within GFF237 at 266.137 kb on - strand, within GFF237 at 266.137 kb on - strand, within GFF237 at 266.137 kb on - strand, within GFF237 at 266.137 kb on - strand, within GFF237 at 266.137 kb on - strand, within GFF237 at 266.149 kb on + strand, within GFF237 at 266.211 kb on + strand, within GFF237 at 266.211 kb on + strand, within GFF237 at 266.211 kb on + strand, within GFF237 at 266.212 kb on - strand, within GFF237 at 266.212 kb on - strand, within GFF237 at 266.212 kb on - strand, within GFF237 at 266.212 kb on - strand, within GFF237 at 266.262 kb on + strand, within GFF237 at 266.262 kb on + strand, within GFF237 at 266.262 kb on + strand, within GFF237 at 266.262 kb on + strand, within GFF237 at 266.262 kb on + strand, within GFF237 at 266.262 kb on + strand, within GFF237 at 266.262 kb on + strand, within GFF237 at 266.263 kb on - strand, within GFF237
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 264,113 + GFF234 0.86 -0.8 264,114 - GFF234 0.87 +0.5 264,114 - GFF234 0.87 -0.7 264,248 + +0.1 264,248 + +0.8 264,248 + -0.1 264,248 + -1.0 264,248 + -2.0 264,248 + +0.4 264,249 - -0.8 264,249 - -0.6 264,371 - +0.7 264,507 + +0.5 264,507 + +0.3 264,507 + -0.8 264,507 + -0.6 264,508 - +1.2 264,508 - -0.5 264,508 - -0.8 264,508 - -0.4 264,508 - -0.3 264,508 - -0.3 264,508 - -0.0 264,508 - -0.8 264,508 - -1.1 264,508 - -0.7 264,523 + -0.8 264,939 - +0.3 264,950 + -1.8 264,950 + -0.7 264,951 - -0.0 264,975 + -0.7 264,975 + -0.5 264,975 + -0.9 264,975 + -0.6 264,976 - -0.7 264,976 - +1.2 264,976 - -0.6 264,976 - +0.4 264,976 - -1.6 264,980 + -0.7 264,980 + +0.4 264,980 + -0.5 264,981 - -0.7 264,981 - -1.2 265,265 + GFF236 0.66 -0.8 265,265 + GFF236 0.66 -0.7 265,265 + GFF236 0.66 -0.8 265,265 + GFF236 0.66 +0.2 265,265 + GFF236 0.66 -1.8 265,265 + GFF236 0.66 -0.0 265,265 + GFF236 0.66 +0.2 265,265 + GFF236 0.66 -0.6 265,266 - GFF236 0.66 -0.3 265,266 - GFF236 0.66 -1.4 265,266 - GFF236 0.66 +0.0 265,266 - GFF236 0.66 +0.4 265,266 - GFF236 0.66 +0.5 265,266 - GFF236 0.66 +0.6 265,266 - GFF236 0.66 -1.4 265,266 - GFF236 0.66 -0.4 265,266 - GFF236 0.66 -0.4 265,266 - GFF236 0.66 -1.1 265,266 - GFF236 0.66 -0.8 265,318 - GFF236 0.80 -2.2 265,355 + GFF236 0.89 -1.0 265,355 + GFF236 0.89 -0.9 265,355 + GFF236 0.89 -0.4 265,355 + GFF236 0.89 -1.1 265,355 + GFF236 0.89 -0.3 265,355 + GFF236 0.89 -0.6 265,356 - GFF236 0.89 -0.6 265,356 - GFF236 0.89 -1.5 265,356 - GFF236 0.89 -0.5 265,356 - GFF236 0.89 -1.5 265,356 - GFF236 0.89 -0.3 265,356 - GFF236 0.89 +0.2 265,394 + -0.5 265,394 + +0.9 265,394 + +0.9 265,509 + -0.8 265,509 + -0.4 265,509 + +1.0 265,509 + -0.8 265,509 + +0.6 265,509 + -0.2 265,509 + -0.0 265,509 + +0.3 265,509 + -0.6 265,509 + +0.7 265,509 + -0.5 265,509 + -0.4 265,509 + +0.6 265,509 + +0.1 265,509 + -1.1 265,509 + -1.8 265,509 + -2.3 265,509 + -0.1 265,510 - -0.6 265,510 - -0.3 265,510 - -0.1 265,510 - +0.1 265,510 - -0.1 265,510 - -0.8 265,510 - -0.4 265,510 - -0.4 265,510 - -1.8 265,510 - -0.6 265,515 + +0.3 265,515 + -1.3 265,515 + -0.0 265,515 + +0.5 265,515 + +0.3 265,515 + +0.2 265,515 + -0.2 265,515 + -2.6 265,515 + -1.1 265,516 - +0.9 265,516 - +0.4 265,516 - -0.5 265,516 - +0.2 265,516 - -1.1 265,516 - -0.5 265,516 - -0.4 265,584 + -2.3 265,584 + +1.4 265,584 + +0.1 265,584 + -0.8 265,584 + -0.5 265,584 + -0.1 265,584 + +0.5 265,584 + -1.0 265,584 + -1.1 265,584 + +0.4 265,584 + +0.2 265,584 + +1.1 265,584 + -0.5 265,584 + -1.0 265,584 + +1.4 265,584 + -1.6 265,585 - -0.2 265,585 - +0.5 265,585 - +0.2 265,585 - -0.3 265,585 - +1.9 265,585 - +0.2 265,585 - -0.4 265,585 - -0.2 265,585 - -0.7 265,585 - -0.4 265,585 - +1.0 265,585 - -0.0 265,585 - -0.6 265,611 + GFF237 0.12 +0.7 265,675 - GFF237 0.17 +0.8 265,675 - GFF237 0.17 -0.0 265,675 - GFF237 0.17 +0.0 265,675 - GFF237 0.17 -0.2 265,680 + GFF237 0.17 +0.7 265,680 + GFF237 0.17 +1.4 265,680 + GFF237 0.17 -0.9 265,680 + GFF237 0.17 +1.5 265,680 + GFF237 0.17 +0.2 265,680 + GFF237 0.17 -0.5 265,680 + GFF237 0.17 +0.1 265,680 + GFF237 0.17 +0.5 265,681 - GFF237 0.17 +1.2 265,681 - GFF237 0.17 -0.0 265,681 - GFF237 0.17 +0.3 265,681 - GFF237 0.17 -0.5 265,681 - GFF237 0.17 -0.8 265,681 - GFF237 0.17 -1.2 265,681 - GFF237 0.17 -0.2 265,681 - GFF237 0.17 +1.8 265,681 - GFF237 0.17 -1.8 265,689 + GFF237 0.18 -1.8 265,689 + GFF237 0.18 +0.6 265,689 + GFF237 0.18 +0.4 265,689 + GFF237 0.18 +0.1 265,689 + GFF237 0.18 -1.4 265,689 + GFF237 0.18 +0.5 265,689 + GFF237 0.18 -2.4 265,689 + GFF237 0.18 +0.5 265,689 + GFF237 0.18 -1.6 265,689 + GFF237 0.18 +0.4 265,690 - GFF237 0.18 -0.6 265,690 - GFF237 0.18 -2.6 265,690 - GFF237 0.18 +0.9 265,690 - GFF237 0.18 +1.1 265,690 - GFF237 0.18 -0.6 265,690 - GFF237 0.18 +0.3 265,690 - GFF237 0.18 +0.3 265,690 - GFF237 0.18 -0.5 265,690 - GFF237 0.18 -0.6 265,690 - GFF237 0.18 -0.5 265,690 - GFF237 0.18 +0.7 266,080 - GFF237 0.50 +1.8 266,080 - GFF237 0.50 +0.4 266,086 + GFF237 0.51 +0.2 266,086 + GFF237 0.51 -0.8 266,086 + GFF237 0.51 +0.2 266,087 - GFF237 0.51 +0.1 266,136 + GFF237 0.55 +0.2 266,136 + GFF237 0.55 +0.6 266,136 + GFF237 0.55 +0.2 266,136 + GFF237 0.55 -0.6 266,136 + GFF237 0.55 -0.4 266,137 - GFF237 0.55 +0.5 266,137 - GFF237 0.55 -1.5 266,137 - GFF237 0.55 -0.0 266,137 - GFF237 0.55 +0.2 266,137 - GFF237 0.55 -0.6 266,137 - GFF237 0.55 +1.2 266,149 + GFF237 0.56 -0.9 266,211 + GFF237 0.61 -0.2 266,211 + GFF237 0.61 +0.5 266,211 + GFF237 0.61 -1.4 266,212 - GFF237 0.61 -1.5 266,212 - GFF237 0.61 -1.1 266,212 - GFF237 0.61 +0.8 266,212 - GFF237 0.61 +0.5 266,262 + GFF237 0.65 +1.0 266,262 + GFF237 0.65 -0.0 266,262 + GFF237 0.65 -0.0 266,262 + GFF237 0.65 +0.1 266,262 + GFF237 0.65 -0.8 266,262 + GFF237 0.65 -0.6 266,262 + GFF237 0.65 +0.2 266,263 - GFF237 0.65 -0.5
Or see this region's nucleotide sequence