Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2233 and GFF2234 are separated by 84 nucleotides GFF2234 and GFF2235 are separated by 7 nucleotides GFF2235 and GFF2236 overlap by 4 nucleotides GFF2236 and GFF2237 are separated by 70 nucleotides
GFF2233 - hypothetical protein, at 294,791 to 295,810
GFF2233
GFF2234 - no description, at 295,895 to 296,095
GFF2234
GFF2235 - hypothetical protein, at 296,103 to 296,834
GFF2235
GFF2236 - hypothetical protein, at 296,831 to 297,247
GFF2236
GFF2237 - Intracellular protease, at 297,318 to 297,896
GFF2237
Position (kb) 296
297 Strain fitness (log2 ratio) -1
0
1
2 at 295.160 kb on + strand, within GFF2233 at 295.209 kb on - strand, within GFF2233 at 295.241 kb on + strand, within GFF2233 at 295.241 kb on + strand, within GFF2233 at 295.242 kb on - strand, within GFF2233 at 295.242 kb on - strand, within GFF2233 at 295.242 kb on - strand, within GFF2233 at 295.298 kb on + strand, within GFF2233 at 295.385 kb on + strand, within GFF2233 at 295.406 kb on + strand, within GFF2233 at 295.406 kb on + strand, within GFF2233 at 296.101 kb on - strand at 296.101 kb on - strand at 296.158 kb on - strand at 296.251 kb on - strand, within GFF2235 at 296.251 kb on - strand, within GFF2235 at 296.251 kb on - strand, within GFF2235 at 296.543 kb on + strand, within GFF2235 at 296.772 kb on + strand at 296.773 kb on - strand at 296.773 kb on - strand at 297.075 kb on + strand, within GFF2236 at 297.210 kb on + strand at 297.210 kb on + strand at 297.295 kb on - strand at 297.442 kb on + strand, within GFF2237 at 297.442 kb on + strand, within GFF2237 at 297.442 kb on + strand, within GFF2237 at 297.443 kb on - strand, within GFF2237 at 297.443 kb on - strand, within GFF2237 at 297.620 kb on - strand, within GFF2237 at 297.659 kb on - strand, within GFF2237
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 295,160 + GFF2233 0.36 -0.7 295,209 - GFF2233 0.41 -0.8 295,241 + GFF2233 0.44 +0.2 295,241 + GFF2233 0.44 +0.7 295,242 - GFF2233 0.44 -0.6 295,242 - GFF2233 0.44 -1.0 295,242 - GFF2233 0.44 -0.0 295,298 + GFF2233 0.50 -0.6 295,385 + GFF2233 0.58 +0.2 295,406 + GFF2233 0.60 -0.8 295,406 + GFF2233 0.60 -0.1 296,101 - -1.0 296,101 - -0.1 296,158 - -0.2 296,251 - GFF2235 0.20 -1.6 296,251 - GFF2235 0.20 +2.7 296,251 - GFF2235 0.20 -1.1 296,543 + GFF2235 0.60 -0.6 296,772 + -0.7 296,773 - -0.7 296,773 - -1.4 297,075 + GFF2236 0.59 -0.1 297,210 + +0.1 297,210 + -0.7 297,295 - +0.7 297,442 + GFF2237 0.21 -0.4 297,442 + GFF2237 0.21 -0.8 297,442 + GFF2237 0.21 -0.3 297,443 - GFF2237 0.22 -1.3 297,443 - GFF2237 0.22 -0.2 297,620 - GFF2237 0.52 -0.4 297,659 - GFF2237 0.59 -1.0
Or see this region's nucleotide sequence