Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1789 and GFF1790 are separated by 13 nucleotides GFF1790 and GFF1791 overlap by 1 nucleotides GFF1791 and GFF1792 are separated by 142 nucleotides
GFF1789 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 449,487 to 450,257
GFF1789
GFF1790 - Hydroxymethylpyrimidine ABC transporter, ATPase component, at 450,271 to 451,143
GFF1790
GFF1791 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 451,143 to 452,177
GFF1791
GFF1792 - Transcriptional regulator, IclR family, at 452,320 to 453,195
GFF1792
Position (kb) 451
452
453 Strain fitness (log2 ratio) -2
-1
0
1 at 450.377 kb on + strand, within GFF1790 at 450.377 kb on + strand, within GFF1790 at 450.377 kb on + strand, within GFF1790 at 450.458 kb on + strand, within GFF1790 at 450.458 kb on + strand, within GFF1790 at 450.458 kb on + strand, within GFF1790 at 450.459 kb on - strand, within GFF1790 at 450.459 kb on - strand, within GFF1790 at 450.744 kb on - strand, within GFF1790 at 451.064 kb on + strand at 451.201 kb on - strand at 451.523 kb on - strand, within GFF1791 at 451.537 kb on + strand, within GFF1791 at 451.841 kb on - strand, within GFF1791 at 451.841 kb on - strand, within GFF1791 at 452.018 kb on - strand, within GFF1791 at 452.116 kb on - strand at 452.116 kb on - strand at 452.351 kb on + strand at 452.352 kb on - strand at 452.352 kb on - strand at 452.465 kb on + strand, within GFF1792 at 452.465 kb on + strand, within GFF1792 at 452.466 kb on - strand, within GFF1792 at 452.466 kb on - strand, within GFF1792 at 452.525 kb on + strand, within GFF1792 at 452.525 kb on + strand, within GFF1792 at 452.526 kb on - strand, within GFF1792 at 452.526 kb on - strand, within GFF1792 at 452.526 kb on - strand, within GFF1792 at 452.729 kb on + strand, within GFF1792 at 452.729 kb on + strand, within GFF1792 at 452.729 kb on + strand, within GFF1792 at 452.729 kb on + strand, within GFF1792 at 452.730 kb on - strand, within GFF1792 at 452.730 kb on - strand, within GFF1792 at 452.730 kb on - strand, within GFF1792 at 452.730 kb on - strand, within GFF1792 at 452.730 kb on - strand, within GFF1792 at 452.801 kb on + strand, within GFF1792 at 452.801 kb on + strand, within GFF1792 at 452.801 kb on + strand, within GFF1792 at 452.802 kb on - strand, within GFF1792 at 452.802 kb on - strand, within GFF1792 at 452.885 kb on + strand, within GFF1792 at 452.885 kb on + strand, within GFF1792 at 452.886 kb on - strand, within GFF1792 at 452.886 kb on - strand, within GFF1792 at 452.918 kb on + strand, within GFF1792 at 453.094 kb on - strand, within GFF1792 at 453.094 kb on - strand, within GFF1792 at 453.094 kb on - strand, within GFF1792 at 453.094 kb on - strand, within GFF1792 at 453.094 kb on - strand, within GFF1792 at 453.094 kb on - strand, within GFF1792 at 453.094 kb on - strand, within GFF1792 at 453.123 kb on + strand at 453.123 kb on + strand at 453.123 kb on + strand at 453.123 kb on + strand at 453.123 kb on + strand at 453.123 kb on + strand at 453.124 kb on - strand at 453.124 kb on - strand at 453.124 kb on - strand at 453.124 kb on - strand at 453.124 kb on - strand at 453.124 kb on - strand at 453.124 kb on - strand at 453.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 450,377 + GFF1790 0.12 +0.5 450,377 + GFF1790 0.12 +1.3 450,377 + GFF1790 0.12 -0.7 450,458 + GFF1790 0.21 +0.3 450,458 + GFF1790 0.21 -0.7 450,458 + GFF1790 0.21 +1.7 450,459 - GFF1790 0.22 -0.3 450,459 - GFF1790 0.22 -0.7 450,744 - GFF1790 0.54 -0.0 451,064 + -0.9 451,201 - +0.0 451,523 - GFF1791 0.37 -0.6 451,537 + GFF1791 0.38 +0.4 451,841 - GFF1791 0.67 +0.8 451,841 - GFF1791 0.67 +0.7 452,018 - GFF1791 0.85 -1.3 452,116 - -1.3 452,116 - -0.5 452,351 + +0.6 452,352 - -0.5 452,352 - -0.9 452,465 + GFF1792 0.17 -0.2 452,465 + GFF1792 0.17 -1.1 452,466 - GFF1792 0.17 -1.2 452,466 - GFF1792 0.17 -1.1 452,525 + GFF1792 0.23 -0.4 452,525 + GFF1792 0.23 -0.6 452,526 - GFF1792 0.24 +0.2 452,526 - GFF1792 0.24 -0.4 452,526 - GFF1792 0.24 -0.2 452,729 + GFF1792 0.47 +0.1 452,729 + GFF1792 0.47 -0.2 452,729 + GFF1792 0.47 -0.4 452,729 + GFF1792 0.47 -0.3 452,730 - GFF1792 0.47 -0.3 452,730 - GFF1792 0.47 +0.5 452,730 - GFF1792 0.47 -1.5 452,730 - GFF1792 0.47 +0.1 452,730 - GFF1792 0.47 -0.4 452,801 + GFF1792 0.55 -1.8 452,801 + GFF1792 0.55 +1.1 452,801 + GFF1792 0.55 -0.2 452,802 - GFF1792 0.55 -0.4 452,802 - GFF1792 0.55 -1.6 452,885 + GFF1792 0.64 -1.1 452,885 + GFF1792 0.64 +0.3 452,886 - GFF1792 0.65 -0.0 452,886 - GFF1792 0.65 +1.0 452,918 + GFF1792 0.68 -0.5 453,094 - GFF1792 0.88 -1.0 453,094 - GFF1792 0.88 -0.3 453,094 - GFF1792 0.88 -0.3 453,094 - GFF1792 0.88 +0.3 453,094 - GFF1792 0.88 -0.0 453,094 - GFF1792 0.88 -1.0 453,094 - GFF1792 0.88 +0.1 453,123 + +0.2 453,123 + +0.7 453,123 + -0.4 453,123 + -2.0 453,123 + -1.1 453,123 + -0.1 453,124 - -1.0 453,124 - -2.7 453,124 - -1.0 453,124 - -0.3 453,124 - -0.3 453,124 - -0.8 453,124 - +0.7 453,124 - -1.7
Or see this region's nucleotide sequence