Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1728 and GFF1729 are separated by 110 nucleotides GFF1729 and GFF1730 are separated by 706 nucleotides
GFF1728 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 387,793 to 389,034
GFF1728
GFF1729 - HIPA PROTEIN, at 389,145 to 390,371
GFF1729
GFF1730 - Dihydrofolate reductase homolog, at 391,078 to 391,734
GFF1730
Position (kb) 389
390
391 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.198 kb on + strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.199 kb on - strand, within GFF1728 at 388.465 kb on + strand, within GFF1728 at 388.465 kb on + strand, within GFF1728 at 388.465 kb on + strand, within GFF1728 at 388.466 kb on - strand, within GFF1728 at 388.466 kb on - strand, within GFF1728 at 388.466 kb on - strand, within GFF1728 at 388.478 kb on - strand, within GFF1728 at 388.478 kb on - strand, within GFF1728 at 388.498 kb on + strand, within GFF1728 at 388.499 kb on - strand, within GFF1728 at 388.499 kb on - strand, within GFF1728 at 388.499 kb on - strand, within GFF1728 at 388.499 kb on - strand, within GFF1728 at 388.499 kb on - strand, within GFF1728 at 388.499 kb on - strand, within GFF1728 at 388.537 kb on + strand, within GFF1728 at 388.537 kb on + strand, within GFF1728 at 388.537 kb on + strand, within GFF1728 at 388.538 kb on - strand, within GFF1728 at 388.538 kb on - strand, within GFF1728 at 388.575 kb on + strand, within GFF1728 at 388.575 kb on + strand, within GFF1728 at 388.575 kb on + strand, within GFF1728 at 388.576 kb on - strand, within GFF1728 at 388.576 kb on - strand, within GFF1728 at 388.576 kb on - strand, within GFF1728 at 388.576 kb on - strand, within GFF1728 at 388.576 kb on - strand, within GFF1728 at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.998 kb on + strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 388.999 kb on - strand at 389.032 kb on + strand at 389.032 kb on + strand at 389.032 kb on + strand at 389.033 kb on - strand at 389.033 kb on - strand at 389.033 kb on - strand at 389.033 kb on - strand at 389.146 kb on + strand at 389.146 kb on + strand at 389.146 kb on + strand at 389.147 kb on - strand at 389.147 kb on - strand at 389.208 kb on + strand at 389.208 kb on + strand at 389.208 kb on + strand at 389.209 kb on - strand at 389.209 kb on - strand at 389.393 kb on - strand, within GFF1729 at 389.404 kb on + strand, within GFF1729 at 389.404 kb on + strand, within GFF1729 at 389.405 kb on - strand, within GFF1729 at 389.405 kb on - strand, within GFF1729 at 389.405 kb on - strand, within GFF1729 at 389.564 kb on - strand, within GFF1729 at 389.572 kb on + strand, within GFF1729 at 389.572 kb on + strand, within GFF1729 at 389.573 kb on - strand, within GFF1729 at 389.573 kb on - strand, within GFF1729 at 389.573 kb on - strand, within GFF1729 at 389.624 kb on + strand, within GFF1729 at 389.624 kb on + strand, within GFF1729 at 389.624 kb on + strand, within GFF1729 at 389.624 kb on + strand, within GFF1729 at 389.624 kb on + strand, within GFF1729 at 389.624 kb on + strand, within GFF1729 at 389.625 kb on - strand, within GFF1729 at 389.625 kb on - strand, within GFF1729 at 389.625 kb on - strand, within GFF1729 at 389.625 kb on - strand, within GFF1729 at 389.696 kb on + strand, within GFF1729 at 389.696 kb on + strand, within GFF1729 at 389.697 kb on - strand, within GFF1729 at 389.697 kb on - strand, within GFF1729 at 389.697 kb on - strand, within GFF1729 at 389.697 kb on - strand, within GFF1729 at 389.704 kb on + strand, within GFF1729 at 389.704 kb on + strand, within GFF1729 at 389.704 kb on + strand, within GFF1729 at 389.705 kb on - strand, within GFF1729 at 389.705 kb on - strand, within GFF1729 at 389.705 kb on - strand, within GFF1729 at 389.752 kb on + strand, within GFF1729 at 389.752 kb on + strand, within GFF1729 at 389.752 kb on + strand, within GFF1729 at 389.752 kb on + strand, within GFF1729 at 389.752 kb on + strand, within GFF1729 at 389.752 kb on + strand, within GFF1729 at 389.752 kb on + strand, within GFF1729 at 389.753 kb on - strand, within GFF1729 at 389.866 kb on + strand, within GFF1729 at 389.866 kb on + strand, within GFF1729 at 389.866 kb on + strand, within GFF1729 at 389.867 kb on - strand, within GFF1729 at 389.867 kb on - strand, within GFF1729 at 389.991 kb on + strand, within GFF1729 at 389.991 kb on + strand, within GFF1729 at 389.991 kb on + strand, within GFF1729 at 389.992 kb on - strand, within GFF1729 at 389.992 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.032 kb on - strand, within GFF1729 at 390.087 kb on + strand, within GFF1729 at 390.087 kb on + strand, within GFF1729 at 390.087 kb on + strand, within GFF1729 at 390.087 kb on + strand, within GFF1729 at 390.087 kb on + strand, within GFF1729 at 390.087 kb on + strand, within GFF1729 at 390.088 kb on - strand, within GFF1729 at 390.122 kb on - strand, within GFF1729 at 390.170 kb on + strand, within GFF1729 at 390.171 kb on - strand, within GFF1729 at 390.280 kb on + strand at 390.280 kb on + strand at 390.281 kb on - strand at 390.281 kb on - strand at 390.281 kb on - strand at 390.281 kb on - strand at 390.281 kb on - strand at 390.349 kb on + strand at 390.349 kb on + strand at 390.349 kb on + strand at 390.350 kb on - strand at 390.350 kb on - strand at 390.350 kb on - strand at 390.352 kb on + strand at 390.352 kb on + strand at 390.353 kb on - strand at 390.353 kb on - strand at 390.442 kb on + strand at 390.443 kb on - strand at 390.443 kb on - strand at 390.443 kb on - strand at 390.628 kb on + strand at 390.628 kb on + strand at 390.629 kb on - strand at 390.629 kb on - strand at 390.629 kb on - strand at 390.653 kb on + strand at 390.653 kb on + strand at 390.654 kb on - strand at 390.654 kb on - strand at 390.654 kb on - strand at 390.654 kb on - strand at 390.654 kb on - strand at 390.708 kb on + strand at 390.709 kb on - strand at 390.709 kb on - strand at 390.791 kb on + strand at 390.791 kb on + strand at 390.791 kb on + strand at 390.792 kb on - strand at 391.145 kb on + strand, within GFF1730 at 391.146 kb on - strand, within GFF1730 at 391.250 kb on + strand, within GFF1730
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 388,198 + GFF1728 0.33 +1.3 388,198 + GFF1728 0.33 -0.5 388,198 + GFF1728 0.33 -0.2 388,198 + GFF1728 0.33 -1.0 388,198 + GFF1728 0.33 -1.1 388,198 + GFF1728 0.33 -0.1 388,198 + GFF1728 0.33 +1.0 388,198 + GFF1728 0.33 -0.5 388,198 + GFF1728 0.33 -0.2 388,198 + GFF1728 0.33 -0.6 388,199 - GFF1728 0.33 -0.2 388,199 - GFF1728 0.33 -0.3 388,199 - GFF1728 0.33 -0.5 388,199 - GFF1728 0.33 -0.3 388,199 - GFF1728 0.33 -0.9 388,199 - GFF1728 0.33 -1.3 388,199 - GFF1728 0.33 +2.1 388,199 - GFF1728 0.33 +2.1 388,199 - GFF1728 0.33 +0.1 388,199 - GFF1728 0.33 -0.1 388,199 - GFF1728 0.33 -2.3 388,465 + GFF1728 0.54 -0.8 388,465 + GFF1728 0.54 -1.3 388,465 + GFF1728 0.54 -0.1 388,466 - GFF1728 0.54 -1.3 388,466 - GFF1728 0.54 -1.2 388,466 - GFF1728 0.54 -1.3 388,478 - GFF1728 0.55 -0.8 388,478 - GFF1728 0.55 -1.0 388,498 + GFF1728 0.57 -1.2 388,499 - GFF1728 0.57 -0.1 388,499 - GFF1728 0.57 +0.3 388,499 - GFF1728 0.57 -0.3 388,499 - GFF1728 0.57 -0.5 388,499 - GFF1728 0.57 -1.3 388,499 - GFF1728 0.57 -0.5 388,537 + GFF1728 0.60 -0.9 388,537 + GFF1728 0.60 +0.7 388,537 + GFF1728 0.60 +0.7 388,538 - GFF1728 0.60 +0.3 388,538 - GFF1728 0.60 +0.0 388,575 + GFF1728 0.63 +0.4 388,575 + GFF1728 0.63 -0.0 388,575 + GFF1728 0.63 +0.5 388,576 - GFF1728 0.63 +0.4 388,576 - GFF1728 0.63 +0.0 388,576 - GFF1728 0.63 +0.3 388,576 - GFF1728 0.63 -0.6 388,576 - GFF1728 0.63 -1.6 388,998 + -0.7 388,998 + -0.3 388,998 + -0.0 388,998 + -0.1 388,998 + -0.5 388,998 + +0.1 388,998 + -0.1 388,998 + -0.5 388,998 + -0.6 388,998 + -0.6 388,998 + -0.5 388,999 - -0.9 388,999 - -0.6 388,999 - +1.2 388,999 - -0.5 388,999 - +0.2 388,999 - +0.7 388,999 - -0.3 388,999 - +0.7 388,999 - +0.2 388,999 - -0.1 388,999 - -0.3 388,999 - +0.4 388,999 - -1.0 388,999 - -0.2 389,032 + -1.3 389,032 + -0.2 389,032 + -1.2 389,033 - -0.2 389,033 - -1.7 389,033 - -0.3 389,033 - +0.2 389,146 + +0.1 389,146 + -1.2 389,146 + -0.8 389,147 - -1.9 389,147 - +0.0 389,208 + -0.1 389,208 + -0.9 389,208 + -0.8 389,209 - -0.5 389,209 - +0.5 389,393 - GFF1729 0.20 +0.2 389,404 + GFF1729 0.21 -0.3 389,404 + GFF1729 0.21 +1.1 389,405 - GFF1729 0.21 +0.3 389,405 - GFF1729 0.21 -1.3 389,405 - GFF1729 0.21 -0.3 389,564 - GFF1729 0.34 -0.3 389,572 + GFF1729 0.35 -1.5 389,572 + GFF1729 0.35 -0.9 389,573 - GFF1729 0.35 +0.3 389,573 - GFF1729 0.35 +0.4 389,573 - GFF1729 0.35 -0.2 389,624 + GFF1729 0.39 +0.7 389,624 + GFF1729 0.39 +0.7 389,624 + GFF1729 0.39 +0.5 389,624 + GFF1729 0.39 -0.8 389,624 + GFF1729 0.39 -0.4 389,624 + GFF1729 0.39 -1.7 389,625 - GFF1729 0.39 -1.1 389,625 - GFF1729 0.39 -0.0 389,625 - GFF1729 0.39 -0.0 389,625 - GFF1729 0.39 -0.4 389,696 + GFF1729 0.45 +0.4 389,696 + GFF1729 0.45 -0.7 389,697 - GFF1729 0.45 +0.7 389,697 - GFF1729 0.45 -1.2 389,697 - GFF1729 0.45 -0.3 389,697 - GFF1729 0.45 -0.7 389,704 + GFF1729 0.46 +0.1 389,704 + GFF1729 0.46 -0.9 389,704 + GFF1729 0.46 -0.9 389,705 - GFF1729 0.46 +1.3 389,705 - GFF1729 0.46 -0.0 389,705 - GFF1729 0.46 -0.4 389,752 + GFF1729 0.49 +1.3 389,752 + GFF1729 0.49 -0.1 389,752 + GFF1729 0.49 +0.0 389,752 + GFF1729 0.49 -0.4 389,752 + GFF1729 0.49 -0.4 389,752 + GFF1729 0.49 -0.1 389,752 + GFF1729 0.49 -0.9 389,753 - GFF1729 0.50 -0.9 389,866 + GFF1729 0.59 -0.3 389,866 + GFF1729 0.59 +1.7 389,866 + GFF1729 0.59 -0.4 389,867 - GFF1729 0.59 -0.2 389,867 - GFF1729 0.59 -1.1 389,991 + GFF1729 0.69 +0.1 389,991 + GFF1729 0.69 +1.7 389,991 + GFF1729 0.69 -0.1 389,992 - GFF1729 0.69 -0.2 389,992 - GFF1729 0.69 -1.0 390,032 - GFF1729 0.72 -0.1 390,032 - GFF1729 0.72 +2.3 390,032 - GFF1729 0.72 -0.8 390,032 - GFF1729 0.72 -1.0 390,032 - GFF1729 0.72 -1.3 390,032 - GFF1729 0.72 -1.0 390,032 - GFF1729 0.72 -2.7 390,032 - GFF1729 0.72 -3.0 390,032 - GFF1729 0.72 +3.1 390,087 + GFF1729 0.77 +0.0 390,087 + GFF1729 0.77 -0.4 390,087 + GFF1729 0.77 -0.2 390,087 + GFF1729 0.77 -0.7 390,087 + GFF1729 0.77 -0.1 390,087 + GFF1729 0.77 +0.6 390,088 - GFF1729 0.77 -0.9 390,122 - GFF1729 0.80 -1.3 390,170 + GFF1729 0.84 +0.2 390,171 - GFF1729 0.84 -0.3 390,280 + -0.4 390,280 + +0.4 390,281 - -0.6 390,281 - -0.3 390,281 - -1.0 390,281 - -0.2 390,281 - -0.2 390,349 + -0.2 390,349 + +0.3 390,349 + -0.2 390,350 - +0.7 390,350 - -0.7 390,350 - -0.2 390,352 + +0.3 390,352 + +0.6 390,353 - -1.5 390,353 - -0.9 390,442 + -2.0 390,443 - +0.1 390,443 - -0.1 390,443 - +0.8 390,628 + -0.5 390,628 + +0.1 390,629 - -0.1 390,629 - -0.5 390,629 - +0.3 390,653 + -0.0 390,653 + -2.5 390,654 - +0.4 390,654 - -0.5 390,654 - -0.3 390,654 - -1.4 390,654 - +0.2 390,708 + +0.0 390,709 - -0.5 390,709 - -0.4 390,791 + +1.3 390,791 + +0.7 390,791 + +1.1 390,792 - -1.7 391,145 + GFF1730 0.10 +0.5 391,146 - GFF1730 0.10 -0.6 391,250 + GFF1730 0.26 +0.1
Or see this region's nucleotide sequence