Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1636 and GFF1637 are separated by 84 nucleotides GFF1637 and GFF1638 overlap by 4 nucleotides
GFF1636 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 256,850 to 260,152
GFF1636
GFF1637 - Polyketide synthase modules and related proteins, at 260,237 to 264,835
GFF1637
GFF1638 - Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 264,832 to 268,113
GFF1638
Position (kb) 260
261
262
263
264
265 Strain fitness (log2 ratio) -1
0
1 at 259.270 kb on + strand, within GFF1636 at 259.350 kb on + strand, within GFF1636 at 259.603 kb on + strand, within GFF1636 at 259.603 kb on + strand, within GFF1636 at 259.604 kb on - strand, within GFF1636 at 259.865 kb on + strand at 259.866 kb on - strand at 259.941 kb on + strand at 259.942 kb on - strand at 260.220 kb on - strand at 260.845 kb on - strand, within GFF1637 at 261.709 kb on - strand, within GFF1637 at 261.807 kb on + strand, within GFF1637 at 261.808 kb on - strand, within GFF1637 at 261.808 kb on - strand, within GFF1637 at 261.891 kb on + strand, within GFF1637 at 262.017 kb on + strand, within GFF1637 at 262.170 kb on + strand, within GFF1637 at 262.171 kb on - strand, within GFF1637 at 262.171 kb on - strand, within GFF1637 at 262.191 kb on + strand, within GFF1637 at 262.233 kb on + strand, within GFF1637 at 262.253 kb on + strand, within GFF1637 at 262.495 kb on - strand, within GFF1637 at 262.917 kb on + strand, within GFF1637 at 262.917 kb on + strand, within GFF1637 at 263.163 kb on + strand, within GFF1637 at 263.243 kb on + strand, within GFF1637 at 263.243 kb on + strand, within GFF1637 at 263.243 kb on + strand, within GFF1637 at 263.244 kb on - strand, within GFF1637 at 263.244 kb on - strand, within GFF1637 at 263.244 kb on - strand, within GFF1637 at 263.244 kb on - strand, within GFF1637 at 263.244 kb on - strand, within GFF1637 at 263.244 kb on - strand, within GFF1637 at 263.283 kb on + strand, within GFF1637 at 263.283 kb on + strand, within GFF1637 at 263.283 kb on + strand, within GFF1637 at 263.284 kb on - strand, within GFF1637 at 263.495 kb on + strand, within GFF1637 at 263.496 kb on - strand, within GFF1637 at 263.661 kb on + strand, within GFF1637 at 263.878 kb on - strand, within GFF1637 at 264.319 kb on - strand, within GFF1637 at 264.432 kb on + strand at 264.456 kb on + strand at 264.556 kb on - strand at 265.143 kb on + strand at 265.346 kb on + strand, within GFF1638 at 265.643 kb on + strand, within GFF1638 at 265.697 kb on + strand, within GFF1638 at 265.697 kb on + strand, within GFF1638 at 265.697 kb on + strand, within GFF1638 at 265.698 kb on - strand, within GFF1638 at 265.698 kb on - strand, within GFF1638 at 265.698 kb on - strand, within GFF1638 at 265.698 kb on - strand, within GFF1638 at 265.799 kb on + strand, within GFF1638 at 265.808 kb on + strand, within GFF1638 at 265.809 kb on - strand, within GFF1638
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 259,270 + GFF1636 0.73 +0.2 259,350 + GFF1636 0.76 -0.1 259,603 + GFF1636 0.83 +0.3 259,603 + GFF1636 0.83 +0.0 259,604 - GFF1636 0.83 +0.4 259,865 + -0.1 259,866 - -0.5 259,941 + +0.4 259,942 - -0.6 260,220 - +0.6 260,845 - GFF1637 0.13 -1.3 261,709 - GFF1637 0.32 +0.0 261,807 + GFF1637 0.34 -0.0 261,808 - GFF1637 0.34 -0.2 261,808 - GFF1637 0.34 +0.1 261,891 + GFF1637 0.36 +0.7 262,017 + GFF1637 0.39 +0.4 262,170 + GFF1637 0.42 +0.2 262,171 - GFF1637 0.42 +1.7 262,171 - GFF1637 0.42 +0.1 262,191 + GFF1637 0.42 +1.4 262,233 + GFF1637 0.43 -0.9 262,253 + GFF1637 0.44 -0.8 262,495 - GFF1637 0.49 +0.2 262,917 + GFF1637 0.58 +0.1 262,917 + GFF1637 0.58 +1.1 263,163 + GFF1637 0.64 -1.6 263,243 + GFF1637 0.65 -1.0 263,243 + GFF1637 0.65 -0.8 263,243 + GFF1637 0.65 +0.6 263,244 - GFF1637 0.65 +0.3 263,244 - GFF1637 0.65 +0.9 263,244 - GFF1637 0.65 -1.0 263,244 - GFF1637 0.65 -0.3 263,244 - GFF1637 0.65 +0.5 263,244 - GFF1637 0.65 -0.0 263,283 + GFF1637 0.66 -1.0 263,283 + GFF1637 0.66 +0.2 263,283 + GFF1637 0.66 +1.2 263,284 - GFF1637 0.66 -0.4 263,495 + GFF1637 0.71 -0.4 263,496 - GFF1637 0.71 +0.1 263,661 + GFF1637 0.74 -0.1 263,878 - GFF1637 0.79 -0.2 264,319 - GFF1637 0.89 +1.1 264,432 + -0.8 264,456 + -1.1 264,556 - -1.0 265,143 + -1.1 265,346 + GFF1638 0.16 -0.6 265,643 + GFF1638 0.25 -0.6 265,697 + GFF1638 0.26 -0.6 265,697 + GFF1638 0.26 -0.5 265,697 + GFF1638 0.26 -0.5 265,698 - GFF1638 0.26 +0.1 265,698 - GFF1638 0.26 +0.2 265,698 - GFF1638 0.26 +0.0 265,698 - GFF1638 0.26 +0.3 265,799 + GFF1638 0.29 +0.9 265,808 + GFF1638 0.30 -0.2 265,809 - GFF1638 0.30 +0.4
Or see this region's nucleotide sequence