Strain Fitness in Variovorax sp. SCN45 around GFF1499

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1497 and GFF1498 overlap by 4 nucleotidesGFF1498 and GFF1499 are separated by 6 nucleotidesGFF1499 and GFF1500 are separated by 130 nucleotides GFF1497 - BUG/TctC family periplasmic protein, at 89,847 to 90,833 GFF1497 GFF1498 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 90,830 to 91,750 GFF1498 GFF1499 - BUG/TctC family periplasmic protein, at 91,757 to 92,743 GFF1499 GFF1500 - Transcriptional regulator, IclR family, at 92,874 to 93,683 GFF1500 Position (kb) 91 92 93Strain fitness (log2 ratio) -2 -1 0 1 2at 90.870 kb on + strandat 90.870 kb on + strandat 90.871 kb on - strandat 91.104 kb on + strand, within GFF1498at 91.104 kb on + strand, within GFF1498at 91.104 kb on + strand, within GFF1498at 91.104 kb on + strand, within GFF1498at 91.105 kb on - strand, within GFF1498at 91.105 kb on - strand, within GFF1498at 91.105 kb on - strand, within GFF1498at 91.214 kb on + strand, within GFF1498at 91.509 kb on + strand, within GFF1498at 91.509 kb on + strand, within GFF1498at 92.001 kb on + strand, within GFF1499at 92.001 kb on + strand, within GFF1499at 92.001 kb on + strand, within GFF1499at 92.002 kb on - strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.124 kb on + strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.125 kb on - strand, within GFF1499at 92.178 kb on + strand, within GFF1499at 92.178 kb on + strand, within GFF1499at 92.179 kb on - strand, within GFF1499at 92.179 kb on - strand, within GFF1499at 92.179 kb on - strand, within GFF1499at 92.265 kb on + strand, within GFF1499at 92.404 kb on - strand, within GFF1499at 92.419 kb on - strand, within GFF1499at 92.469 kb on + strand, within GFF1499at 92.470 kb on - strand, within GFF1499at 92.653 kb on - strandat 92.653 kb on - strandat 92.737 kb on + strandat 92.737 kb on + strandat 92.737 kb on + strandat 92.738 kb on - strandat 92.826 kb on + strandat 92.826 kb on + strandat 92.826 kb on + strandat 92.826 kb on + strandat 92.827 kb on - strandat 92.827 kb on - strandat 93.117 kb on + strand, within GFF1500at 93.117 kb on + strand, within GFF1500at 93.118 kb on - strand, within GFF1500at 93.118 kb on - strand, within GFF1500at 93.267 kb on + strand, within GFF1500at 93.267 kb on + strand, within GFF1500at 93.267 kb on + strand, within GFF1500at 93.267 kb on + strand, within GFF1500at 93.267 kb on + strand, within GFF1500at 93.267 kb on + strand, within GFF1500at 93.268 kb on - strand, within GFF1500at 93.268 kb on - strand, within GFF1500at 93.268 kb on - strand, within GFF1500at 93.420 kb on + strand, within GFF1500at 93.420 kb on + strand, within GFF1500at 93.420 kb on + strand, within GFF1500at 93.421 kb on - strand, within GFF1500at 93.421 kb on - strand, within GFF1500at 93.444 kb on + strand, within GFF1500at 93.444 kb on + strand, within GFF1500at 93.445 kb on - strand, within GFF1500at 93.445 kb on - strand, within GFF1500at 93.445 kb on - strand, within GFF1500at 93.445 kb on - strand, within GFF1500at 93.445 kb on - strand, within GFF1500at 93.621 kb on + strandat 93.622 kb on - strandat 93.681 kb on + strandat 93.682 kb on - strandat 93.682 kb on - strandat 93.682 kb on - strandat 93.682 kb on - strandat 93.682 kb on - strandat 93.682 kb on - strandat 93.727 kb on - strandat 93.727 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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90,870 + -0.4
90,870 + +0.1
90,871 - -0.7
91,104 + GFF1498 0.30 +0.9
91,104 + GFF1498 0.30 -0.2
91,104 + GFF1498 0.30 +0.2
91,104 + GFF1498 0.30 -1.1
91,105 - GFF1498 0.30 -1.8
91,105 - GFF1498 0.30 +0.2
91,105 - GFF1498 0.30 -1.2
91,214 + GFF1498 0.42 -0.4
91,509 + GFF1498 0.74 +1.1
91,509 + GFF1498 0.74 -0.4
92,001 + GFF1499 0.25 -0.5
92,001 + GFF1499 0.25 -0.4
92,001 + GFF1499 0.25 -0.3
92,002 - GFF1499 0.25 +0.1
92,124 + GFF1499 0.37 -0.4
92,124 + GFF1499 0.37 -0.0
92,124 + GFF1499 0.37 -0.7
92,124 + GFF1499 0.37 -0.7
92,124 + GFF1499 0.37 +0.4
92,124 + GFF1499 0.37 +0.2
92,124 + GFF1499 0.37 +0.2
92,124 + GFF1499 0.37 -0.2
92,124 + GFF1499 0.37 -0.1
92,124 + GFF1499 0.37 -0.2
92,124 + GFF1499 0.37 +1.1
92,124 + GFF1499 0.37 +1.1
92,124 + GFF1499 0.37 -1.7
92,124 + GFF1499 0.37 -0.1
92,124 + GFF1499 0.37 -0.3
92,125 - GFF1499 0.37 +0.4
92,125 - GFF1499 0.37 -0.9
92,125 - GFF1499 0.37 -0.1
92,125 - GFF1499 0.37 +0.2
92,125 - GFF1499 0.37 -1.4
92,125 - GFF1499 0.37 -0.2
92,125 - GFF1499 0.37 -0.4
92,125 - GFF1499 0.37 -0.4
92,178 + GFF1499 0.43 -0.5
92,178 + GFF1499 0.43 -0.3
92,179 - GFF1499 0.43 +0.1
92,179 - GFF1499 0.43 -0.0
92,179 - GFF1499 0.43 -0.1
92,265 + GFF1499 0.51 +0.6
92,404 - GFF1499 0.66 +0.9
92,419 - GFF1499 0.67 +0.5
92,469 + GFF1499 0.72 -0.5
92,470 - GFF1499 0.72 -0.3
92,653 - +1.6
92,653 - -0.5
92,737 + -0.7
92,737 + +0.3
92,737 + -1.2
92,738 - -0.1
92,826 + +0.1
92,826 + -0.4
92,826 + +0.7
92,826 + +1.2
92,827 - -0.1
92,827 - +2.1
93,117 + GFF1500 0.30 +0.2
93,117 + GFF1500 0.30 +0.4
93,118 - GFF1500 0.30 -0.2
93,118 - GFF1500 0.30 -0.5
93,267 + GFF1500 0.49 -0.1
93,267 + GFF1500 0.49 -0.4
93,267 + GFF1500 0.49 +0.8
93,267 + GFF1500 0.49 -0.3
93,267 + GFF1500 0.49 +1.6
93,267 + GFF1500 0.49 -0.4
93,268 - GFF1500 0.49 -0.7
93,268 - GFF1500 0.49 -1.2
93,268 - GFF1500 0.49 -1.6
93,420 + GFF1500 0.67 +0.1
93,420 + GFF1500 0.67 +0.4
93,420 + GFF1500 0.67 -0.1
93,421 - GFF1500 0.68 +0.0
93,421 - GFF1500 0.68 -0.9
93,444 + GFF1500 0.70 -0.9
93,444 + GFF1500 0.70 +0.3
93,445 - GFF1500 0.70 -1.6
93,445 - GFF1500 0.70 +0.6
93,445 - GFF1500 0.70 +1.8
93,445 - GFF1500 0.70 -0.9
93,445 - GFF1500 0.70 +0.3
93,621 + -0.4
93,622 - +0.3
93,681 + -1.2
93,682 - +1.0
93,682 - -0.2
93,682 - +0.3
93,682 - -0.7
93,682 - -0.9
93,682 - -0.3
93,727 - +0.3
93,727 - -0.4

Or see this region's nucleotide sequence