Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1482 and GFF1483 overlap by 13 nucleotides GFF1483 and GFF1484 are separated by 101 nucleotides GFF1484 and GFF1485 are separated by 221 nucleotides
GFF1482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 74,871 to 75,632
GFF1482
GFF1483 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 75,620 to 76,423
GFF1483
GFF1484 - Transcriptional regulator, LysR family, at 76,525 to 77,406
GFF1484
GFF1485 - Transcriptional regulator, AraC family, at 77,628 to 78,449
GFF1485
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 75.543 kb on + strand, within GFF1482 at 75.740 kb on - strand, within GFF1483 at 75.976 kb on - strand, within GFF1483 at 76.405 kb on + strand at 76.485 kb on - strand at 76.505 kb on + strand at 76.506 kb on - strand at 76.513 kb on + strand at 76.513 kb on + strand at 76.514 kb on - strand at 76.514 kb on - strand at 76.516 kb on + strand at 76.532 kb on + strand at 76.533 kb on - strand at 76.813 kb on + strand, within GFF1484 at 76.813 kb on + strand, within GFF1484 at 76.813 kb on + strand, within GFF1484 at 76.815 kb on + strand, within GFF1484 at 76.815 kb on + strand, within GFF1484 at 76.815 kb on + strand, within GFF1484 at 76.815 kb on + strand, within GFF1484 at 76.815 kb on + strand, within GFF1484 at 76.816 kb on - strand, within GFF1484 at 76.816 kb on - strand, within GFF1484 at 76.816 kb on - strand, within GFF1484 at 76.831 kb on + strand, within GFF1484 at 76.918 kb on + strand, within GFF1484 at 76.958 kb on + strand, within GFF1484 at 77.003 kb on + strand, within GFF1484 at 77.004 kb on - strand, within GFF1484 at 77.004 kb on - strand, within GFF1484 at 77.114 kb on - strand, within GFF1484 at 77.267 kb on + strand, within GFF1484 at 77.268 kb on - strand, within GFF1484 at 77.478 kb on + strand at 77.478 kb on + strand at 77.478 kb on + strand at 77.478 kb on + strand at 77.478 kb on + strand at 77.479 kb on - strand at 77.684 kb on + strand at 77.684 kb on + strand at 77.684 kb on + strand at 77.686 kb on + strand at 77.686 kb on + strand at 77.687 kb on - strand at 77.687 kb on - strand at 77.961 kb on + strand, within GFF1485 at 78.076 kb on + strand, within GFF1485 at 78.077 kb on - strand, within GFF1485 at 78.187 kb on + strand, within GFF1485 at 78.188 kb on - strand, within GFF1485 at 78.332 kb on + strand, within GFF1485
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 75,543 + GFF1482 0.88 +0.3 75,740 - GFF1483 0.15 +1.6 75,976 - GFF1483 0.44 -0.9 76,405 + -0.4 76,485 - -0.2 76,505 + -0.3 76,506 - -0.8 76,513 + +1.3 76,513 + +0.6 76,514 - -0.9 76,514 - -0.1 76,516 + -0.3 76,532 + -0.6 76,533 - +0.4 76,813 + GFF1484 0.33 +0.4 76,813 + GFF1484 0.33 -0.2 76,813 + GFF1484 0.33 -0.8 76,815 + GFF1484 0.33 +1.6 76,815 + GFF1484 0.33 -2.4 76,815 + GFF1484 0.33 -0.4 76,815 + GFF1484 0.33 -1.2 76,815 + GFF1484 0.33 -0.9 76,816 - GFF1484 0.33 -0.2 76,816 - GFF1484 0.33 -2.2 76,816 - GFF1484 0.33 -1.4 76,831 + GFF1484 0.35 -0.2 76,918 + GFF1484 0.45 -0.5 76,958 + GFF1484 0.49 -0.6 77,003 + GFF1484 0.54 -0.3 77,004 - GFF1484 0.54 -1.1 77,004 - GFF1484 0.54 +2.2 77,114 - GFF1484 0.67 +0.1 77,267 + GFF1484 0.84 -0.4 77,268 - GFF1484 0.84 +1.6 77,478 + -0.7 77,478 + -0.3 77,478 + -0.4 77,478 + -0.9 77,478 + -0.7 77,479 - -0.8 77,684 + -0.8 77,684 + -0.9 77,684 + -0.0 77,686 + -0.5 77,686 + -0.1 77,687 - -0.7 77,687 - +0.1 77,961 + GFF1485 0.41 -1.1 78,076 + GFF1485 0.55 -0.1 78,077 - GFF1485 0.55 -0.9 78,187 + GFF1485 0.68 -1.1 78,188 - GFF1485 0.68 +1.6 78,332 + GFF1485 0.86 +0.4
Or see this region's nucleotide sequence