Strain Fitness in Variovorax sp. SCN45 around GFF1484

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1482 and GFF1483 overlap by 13 nucleotidesGFF1483 and GFF1484 are separated by 101 nucleotidesGFF1484 and GFF1485 are separated by 221 nucleotides GFF1482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 74,871 to 75,632 GFF1482 GFF1483 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 75,620 to 76,423 GFF1483 GFF1484 - Transcriptional regulator, LysR family, at 76,525 to 77,406 GFF1484 GFF1485 - Transcriptional regulator, AraC family, at 77,628 to 78,449 GFF1485 Position (kb) 76 77 78Strain fitness (log2 ratio) -2 -1 0 1 2at 75.543 kb on + strand, within GFF1482at 75.740 kb on - strand, within GFF1483at 75.976 kb on - strand, within GFF1483at 76.405 kb on + strandat 76.485 kb on - strandat 76.505 kb on + strandat 76.506 kb on - strandat 76.513 kb on + strandat 76.513 kb on + strandat 76.514 kb on - strandat 76.514 kb on - strandat 76.516 kb on + strandat 76.532 kb on + strandat 76.533 kb on - strandat 76.813 kb on + strand, within GFF1484at 76.813 kb on + strand, within GFF1484at 76.813 kb on + strand, within GFF1484at 76.815 kb on + strand, within GFF1484at 76.815 kb on + strand, within GFF1484at 76.815 kb on + strand, within GFF1484at 76.815 kb on + strand, within GFF1484at 76.815 kb on + strand, within GFF1484at 76.816 kb on - strand, within GFF1484at 76.816 kb on - strand, within GFF1484at 76.816 kb on - strand, within GFF1484at 76.831 kb on + strand, within GFF1484at 76.918 kb on + strand, within GFF1484at 76.958 kb on + strand, within GFF1484at 77.003 kb on + strand, within GFF1484at 77.004 kb on - strand, within GFF1484at 77.004 kb on - strand, within GFF1484at 77.114 kb on - strand, within GFF1484at 77.267 kb on + strand, within GFF1484at 77.268 kb on - strand, within GFF1484at 77.478 kb on + strandat 77.478 kb on + strandat 77.478 kb on + strandat 77.478 kb on + strandat 77.478 kb on + strandat 77.479 kb on - strandat 77.684 kb on + strandat 77.684 kb on + strandat 77.684 kb on + strandat 77.686 kb on + strandat 77.686 kb on + strandat 77.687 kb on - strandat 77.687 kb on - strandat 77.961 kb on + strand, within GFF1485at 78.076 kb on + strand, within GFF1485at 78.077 kb on - strand, within GFF1485at 78.187 kb on + strand, within GFF1485at 78.188 kb on - strand, within GFF1485at 78.332 kb on + strand, within GFF1485

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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75,543 + GFF1482 0.88 +0.3
75,740 - GFF1483 0.15 +1.6
75,976 - GFF1483 0.44 -0.9
76,405 + -0.4
76,485 - -0.2
76,505 + -0.3
76,506 - -0.8
76,513 + +1.3
76,513 + +0.6
76,514 - -0.9
76,514 - -0.1
76,516 + -0.3
76,532 + -0.6
76,533 - +0.4
76,813 + GFF1484 0.33 +0.4
76,813 + GFF1484 0.33 -0.2
76,813 + GFF1484 0.33 -0.8
76,815 + GFF1484 0.33 +1.6
76,815 + GFF1484 0.33 -2.4
76,815 + GFF1484 0.33 -0.4
76,815 + GFF1484 0.33 -1.2
76,815 + GFF1484 0.33 -0.9
76,816 - GFF1484 0.33 -0.2
76,816 - GFF1484 0.33 -2.2
76,816 - GFF1484 0.33 -1.4
76,831 + GFF1484 0.35 -0.2
76,918 + GFF1484 0.45 -0.5
76,958 + GFF1484 0.49 -0.6
77,003 + GFF1484 0.54 -0.3
77,004 - GFF1484 0.54 -1.1
77,004 - GFF1484 0.54 +2.2
77,114 - GFF1484 0.67 +0.1
77,267 + GFF1484 0.84 -0.4
77,268 - GFF1484 0.84 +1.6
77,478 + -0.7
77,478 + -0.3
77,478 + -0.4
77,478 + -0.9
77,478 + -0.7
77,479 - -0.8
77,684 + -0.8
77,684 + -0.9
77,684 + -0.0
77,686 + -0.5
77,686 + -0.1
77,687 - -0.7
77,687 - +0.1
77,961 + GFF1485 0.41 -1.1
78,076 + GFF1485 0.55 -0.1
78,077 - GFF1485 0.55 -0.9
78,187 + GFF1485 0.68 -1.1
78,188 - GFF1485 0.68 +1.6
78,332 + GFF1485 0.86 +0.4

Or see this region's nucleotide sequence