Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF918 and GFF919 are separated by 129 nucleotides GFF919 and GFF920 are separated by 20 nucleotides
GFF918 - Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18), at 230,291 to 231,646
GFF918
GFF919 - hypothetical protein, at 231,776 to 232,744
GFF919
GFF920 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily, at 232,765 to 233,994
GFF920
Position (kb)
231
232
233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 231.228 kb on + strand, within GFF918 at 231.258 kb on + strand, within GFF918 at 231.259 kb on - strand, within GFF918 at 231.406 kb on - strand, within GFF918 at 231.525 kb on + strand at 231.526 kb on - strand at 231.526 kb on - strand at 231.528 kb on + strand at 231.528 kb on + strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.737 kb on + strand at 231.737 kb on + strand at 231.737 kb on + strand at 231.737 kb on + strand at 231.737 kb on + strand at 231.738 kb on - strand at 231.738 kb on - strand at 231.738 kb on - strand at 231.881 kb on + strand, within GFF919 at 231.881 kb on + strand, within GFF919 at 231.881 kb on + strand, within GFF919 at 231.881 kb on + strand, within GFF919 at 231.881 kb on + strand, within GFF919 at 231.882 kb on - strand, within GFF919 at 231.882 kb on - strand, within GFF919 at 231.882 kb on - strand, within GFF919 at 231.911 kb on + strand, within GFF919 at 231.912 kb on - strand, within GFF919 at 232.040 kb on + strand, within GFF919 at 232.040 kb on + strand, within GFF919 at 232.040 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.115 kb on + strand, within GFF919 at 232.116 kb on - strand, within GFF919 at 232.116 kb on - strand, within GFF919 at 232.190 kb on + strand, within GFF919 at 232.190 kb on + strand, within GFF919 at 232.190 kb on + strand, within GFF919 at 232.190 kb on + strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.191 kb on - strand, within GFF919 at 232.265 kb on + strand, within GFF919 at 232.265 kb on + strand, within GFF919 at 232.265 kb on + strand, within GFF919 at 232.266 kb on - strand, within GFF919 at 232.266 kb on - strand, within GFF919 at 232.322 kb on + strand, within GFF919 at 232.323 kb on - strand, within GFF919 at 232.323 kb on - strand, within GFF919 at 232.323 kb on - strand, within GFF919 at 232.443 kb on - strand, within GFF919 at 233.089 kb on + strand, within GFF920 at 233.090 kb on - strand, within GFF920 at 233.090 kb on - strand, within GFF920 at 233.099 kb on - strand, within GFF920 at 233.224 kb on + strand, within GFF920 at 233.224 kb on + strand, within GFF920 at 233.224 kb on + strand, within GFF920 at 233.303 kb on - strand, within GFF920 at 233.383 kb on + strand, within GFF920 at 233.384 kb on - strand, within GFF920 at 233.384 kb on - strand, within GFF920 at 233.458 kb on + strand, within GFF920 at 233.472 kb on + strand, within GFF920
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 231,228 + GFF918 0.69 -1.4 231,258 + GFF918 0.71 -1.0 231,259 - GFF918 0.71 +0.1 231,406 - GFF918 0.82 -0.2 231,525 + +1.1 231,526 - +0.0 231,526 - -0.6 231,528 + -0.3 231,528 + +0.1 231,529 - -1.7 231,529 - -0.2 231,529 - -0.8 231,529 - +0.4 231,529 - -1.8 231,529 - +1.5 231,529 - -0.7 231,529 - -2.0 231,737 + +0.2 231,737 + -0.4 231,737 + +0.1 231,737 + -0.4 231,737 + +0.1 231,738 - +1.1 231,738 - -0.2 231,738 - +0.1 231,881 + GFF919 0.11 -0.4 231,881 + GFF919 0.11 -0.4 231,881 + GFF919 0.11 -0.5 231,881 + GFF919 0.11 +0.2 231,881 + GFF919 0.11 +2.1 231,882 - GFF919 0.11 -1.0 231,882 - GFF919 0.11 +1.2 231,882 - GFF919 0.11 -0.6 231,911 + GFF919 0.14 +0.3 231,912 - GFF919 0.14 -0.4 232,040 + GFF919 0.27 +1.6 232,040 + GFF919 0.27 -0.6 232,040 + GFF919 0.27 +0.1 232,115 + GFF919 0.35 -0.6 232,115 + GFF919 0.35 +1.2 232,115 + GFF919 0.35 +1.0 232,115 + GFF919 0.35 -1.2 232,115 + GFF919 0.35 -0.6 232,115 + GFF919 0.35 -0.4 232,115 + GFF919 0.35 -0.0 232,115 + GFF919 0.35 -0.5 232,116 - GFF919 0.35 -1.1 232,116 - GFF919 0.35 +1.4 232,190 + GFF919 0.43 -0.1 232,190 + GFF919 0.43 +0.7 232,190 + GFF919 0.43 -0.6 232,190 + GFF919 0.43 +0.4 232,191 - GFF919 0.43 -1.0 232,191 - GFF919 0.43 -0.6 232,191 - GFF919 0.43 +1.6 232,191 - GFF919 0.43 -0.3 232,191 - GFF919 0.43 +0.2 232,191 - GFF919 0.43 +0.6 232,191 - GFF919 0.43 +0.8 232,191 - GFF919 0.43 -2.5 232,265 + GFF919 0.50 -1.1 232,265 + GFF919 0.50 +1.1 232,265 + GFF919 0.50 -2.0 232,266 - GFF919 0.51 +1.4 232,266 - GFF919 0.51 +1.0 232,322 + GFF919 0.56 -0.5 232,323 - GFF919 0.56 -0.3 232,323 - GFF919 0.56 +0.1 232,323 - GFF919 0.56 -0.2 232,443 - GFF919 0.69 -0.2 233,089 + GFF920 0.26 -0.2 233,090 - GFF920 0.26 -0.4 233,090 - GFF920 0.26 -0.4 233,099 - GFF920 0.27 -0.4 233,224 + GFF920 0.37 +0.2 233,224 + GFF920 0.37 -0.6 233,224 + GFF920 0.37 +1.1 233,303 - GFF920 0.44 +0.0 233,383 + GFF920 0.50 -0.2 233,384 - GFF920 0.50 -0.4 233,384 - GFF920 0.50 +0.1 233,458 + GFF920 0.56 -2.4 233,472 + GFF920 0.57 -0.8
Or see this region's nucleotide sequence