Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF916 and GFF917 are separated by 54 nucleotides GFF917 and GFF918 are separated by 25 nucleotides
GFF916 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 227,491 to 229,110
GFF916
GFF917 - hypothetical protein, at 229,165 to 230,265
GFF917
GFF918 - Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18), at 230,291 to 231,646
GFF918
Position (kb) 229
230
231 Strain fitness (log2 ratio) -2
-1
0
1
2 at 228.314 kb on + strand, within GFF916 at 228.426 kb on - strand, within GFF916 at 228.452 kb on + strand, within GFF916 at 228.452 kb on + strand, within GFF916 at 228.453 kb on - strand, within GFF916 at 228.483 kb on - strand, within GFF916 at 228.716 kb on + strand, within GFF916 at 228.717 kb on - strand, within GFF916 at 228.800 kb on + strand, within GFF916 at 228.800 kb on + strand, within GFF916 at 228.801 kb on - strand, within GFF916 at 228.801 kb on - strand, within GFF916 at 228.967 kb on + strand at 228.968 kb on - strand at 228.968 kb on - strand at 229.102 kb on + strand at 229.103 kb on - strand at 229.176 kb on - strand at 229.176 kb on - strand at 229.644 kb on - strand, within GFF917 at 229.644 kb on - strand, within GFF917 at 229.973 kb on + strand, within GFF917 at 229.974 kb on - strand, within GFF917 at 229.974 kb on - strand, within GFF917 at 230.157 kb on - strand at 230.201 kb on + strand at 230.679 kb on + strand, within GFF918 at 230.679 kb on + strand, within GFF918 at 230.679 kb on + strand, within GFF918 at 230.679 kb on + strand, within GFF918 at 230.680 kb on - strand, within GFF918 at 230.680 kb on - strand, within GFF918 at 230.685 kb on + strand, within GFF918 at 230.685 kb on + strand, within GFF918 at 230.685 kb on + strand, within GFF918 at 230.686 kb on - strand, within GFF918 at 231.228 kb on + strand, within GFF918 at 231.258 kb on + strand, within GFF918 at 231.259 kb on - strand, within GFF918
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 228,314 + GFF916 0.51 -0.4 228,426 - GFF916 0.58 -0.6 228,452 + GFF916 0.59 -0.1 228,452 + GFF916 0.59 -1.4 228,453 - GFF916 0.59 -1.6 228,483 - GFF916 0.61 -1.0 228,716 + GFF916 0.76 -0.1 228,717 - GFF916 0.76 -0.2 228,800 + GFF916 0.81 -0.1 228,800 + GFF916 0.81 -0.4 228,801 - GFF916 0.81 -1.0 228,801 - GFF916 0.81 -0.8 228,967 + -1.8 228,968 - +0.4 228,968 - +0.0 229,102 + +0.8 229,103 - -0.4 229,176 - +0.2 229,176 - -1.1 229,644 - GFF917 0.44 -1.1 229,644 - GFF917 0.44 -1.0 229,973 + GFF917 0.73 -0.9 229,974 - GFF917 0.73 -0.5 229,974 - GFF917 0.73 -2.0 230,157 - -0.6 230,201 + -1.6 230,679 + GFF918 0.29 +0.1 230,679 + GFF918 0.29 +0.3 230,679 + GFF918 0.29 +1.4 230,679 + GFF918 0.29 +1.9 230,680 - GFF918 0.29 -1.8 230,680 - GFF918 0.29 -0.6 230,685 + GFF918 0.29 -1.3 230,685 + GFF918 0.29 -0.5 230,685 + GFF918 0.29 -1.3 230,686 - GFF918 0.29 -0.6 231,228 + GFF918 0.69 -1.4 231,258 + GFF918 0.71 -1.0 231,259 - GFF918 0.71 +0.1
Or see this region's nucleotide sequence