Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF914 and GFF915 are separated by 1 nucleotides GFF915 and GFF916 are separated by 18 nucleotides GFF916 and GFF917 are separated by 54 nucleotides
GFF914 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 225,711 to 226,526
GFF914
GFF915 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 226,528 to 227,472
GFF915
GFF916 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 227,491 to 229,110
GFF916
GFF917 - hypothetical protein, at 229,165 to 230,265
GFF917
Position (kb) 227
228
229
230 Strain fitness (log2 ratio) -2
-1
0
1 at 226.505 kb on + strand at 226.529 kb on + strand at 226.529 kb on + strand at 226.562 kb on + strand at 226.563 kb on - strand at 226.595 kb on + strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.838 kb on + strand, within GFF915 at 226.838 kb on + strand, within GFF915 at 226.839 kb on - strand, within GFF915 at 226.839 kb on - strand, within GFF915 at 226.934 kb on + strand, within GFF915 at 226.935 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 227.279 kb on + strand, within GFF915 at 227.279 kb on + strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.462 kb on + strand at 227.463 kb on - strand at 227.771 kb on + strand, within GFF916 at 227.822 kb on + strand, within GFF916 at 227.838 kb on - strand, within GFF916 at 227.930 kb on + strand, within GFF916 at 228.053 kb on + strand, within GFF916 at 228.053 kb on + strand, within GFF916 at 228.056 kb on + strand, within GFF916 at 228.314 kb on + strand, within GFF916 at 228.426 kb on - strand, within GFF916 at 228.452 kb on + strand, within GFF916 at 228.452 kb on + strand, within GFF916 at 228.453 kb on - strand, within GFF916 at 228.483 kb on - strand, within GFF916 at 228.716 kb on + strand, within GFF916 at 228.717 kb on - strand, within GFF916 at 228.800 kb on + strand, within GFF916 at 228.800 kb on + strand, within GFF916 at 228.801 kb on - strand, within GFF916 at 228.801 kb on - strand, within GFF916 at 228.967 kb on + strand at 228.968 kb on - strand at 228.968 kb on - strand at 229.102 kb on + strand at 229.103 kb on - strand at 229.176 kb on - strand at 229.176 kb on - strand at 229.644 kb on - strand, within GFF917 at 229.644 kb on - strand, within GFF917 at 229.973 kb on + strand, within GFF917 at 229.974 kb on - strand, within GFF917 at 229.974 kb on - strand, within GFF917
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 226,505 + +0.4 226,529 + -0.2 226,529 + +1.2 226,562 + -1.1 226,563 - -0.2 226,595 + -0.2 226,596 - -1.2 226,596 - +0.2 226,596 - +0.6 226,596 - +0.5 226,838 + GFF915 0.33 +0.4 226,838 + GFF915 0.33 -0.7 226,839 - GFF915 0.33 -0.8 226,839 - GFF915 0.33 +0.1 226,934 + GFF915 0.43 -0.2 226,935 - GFF915 0.43 -0.7 226,974 - GFF915 0.47 +0.2 226,974 - GFF915 0.47 -0.5 226,974 - GFF915 0.47 -0.3 227,279 + GFF915 0.79 -0.8 227,279 + GFF915 0.79 -0.1 227,280 - GFF915 0.80 +0.1 227,280 - GFF915 0.80 +0.5 227,280 - GFF915 0.80 -0.2 227,280 - GFF915 0.80 +0.3 227,462 + -0.6 227,463 - -0.2 227,771 + GFF916 0.17 -0.8 227,822 + GFF916 0.20 -0.3 227,838 - GFF916 0.21 +0.4 227,930 + GFF916 0.27 -0.3 228,053 + GFF916 0.35 -0.2 228,053 + GFF916 0.35 -2.2 228,056 + GFF916 0.35 -0.2 228,314 + GFF916 0.51 -0.4 228,426 - GFF916 0.58 -0.6 228,452 + GFF916 0.59 -0.1 228,452 + GFF916 0.59 -1.4 228,453 - GFF916 0.59 -1.6 228,483 - GFF916 0.61 -1.0 228,716 + GFF916 0.76 -0.1 228,717 - GFF916 0.76 -0.2 228,800 + GFF916 0.81 -0.1 228,800 + GFF916 0.81 -0.4 228,801 - GFF916 0.81 -1.0 228,801 - GFF916 0.81 -0.8 228,967 + -1.8 228,968 - +0.4 228,968 - +0.0 229,102 + +0.8 229,103 - -0.4 229,176 - +0.2 229,176 - -1.1 229,644 - GFF917 0.44 -1.1 229,644 - GFF917 0.44 -1.0 229,973 + GFF917 0.73 -0.9 229,974 - GFF917 0.73 -0.5 229,974 - GFF917 0.73 -2.0
Or see this region's nucleotide sequence