Strain Fitness in Variovorax sp. SCN45 around GFF747

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF746 and GFF747 are separated by 35 nucleotidesGFF747 and GFF748 are separated by 15 nucleotidesGFF748 and GFF749 are separated by 2 nucleotides GFF746 - BUG/TctC family periplasmic protein, at 9,413 to 10,393 GFF746 GFF747 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 10,429 to 11,733 GFF747 GFF748 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase (EC 4.1.3.17), at 11,749 to 12,447 GFF748 GFF749 - 4-oxalomesaconate hydratase (EC 4.2.1.83), at 12,450 to 13,478 GFF749 Position (kb) 10 11 12Strain fitness (log2 ratio) -2 -1 0 1at 9.454 kb on - strandat 9.630 kb on + strand, within GFF746at 9.834 kb on + strand, within GFF746at 9.835 kb on - strand, within GFF746at 9.835 kb on - strand, within GFF746at 10.060 kb on - strand, within GFF746at 10.122 kb on + strand, within GFF746at 10.122 kb on + strand, within GFF746at 10.123 kb on - strand, within GFF746at 10.123 kb on - strand, within GFF746at 10.123 kb on - strand, within GFF746at 10.123 kb on - strand, within GFF746at 10.386 kb on + strandat 10.497 kb on - strandat 10.562 kb on + strand, within GFF747at 10.562 kb on + strand, within GFF747at 10.562 kb on + strand, within GFF747at 10.562 kb on + strand, within GFF747at 10.562 kb on + strand, within GFF747at 10.563 kb on - strand, within GFF747at 10.563 kb on - strand, within GFF747at 10.563 kb on - strand, within GFF747at 10.585 kb on + strand, within GFF747at 10.586 kb on - strand, within GFF747at 10.629 kb on - strand, within GFF747at 10.629 kb on - strand, within GFF747at 10.778 kb on + strand, within GFF747at 10.812 kb on - strand, within GFF747at 10.910 kb on + strand, within GFF747at 10.910 kb on + strand, within GFF747at 10.911 kb on - strand, within GFF747at 10.911 kb on - strand, within GFF747at 10.911 kb on - strand, within GFF747at 10.911 kb on - strand, within GFF747at 10.946 kb on + strand, within GFF747at 10.946 kb on + strand, within GFF747at 10.946 kb on + strand, within GFF747at 10.946 kb on + strand, within GFF747at 10.946 kb on + strand, within GFF747at 10.946 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.949 kb on + strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 10.950 kb on - strand, within GFF747at 11.204 kb on + strand, within GFF747at 11.244 kb on - strand, within GFF747at 11.531 kb on + strand, within GFF747at 11.531 kb on + strand, within GFF747at 11.532 kb on - strand, within GFF747at 11.532 kb on - strand, within GFF747at 11.567 kb on + strand, within GFF747at 11.567 kb on + strand, within GFF747at 11.568 kb on - strand, within GFF747at 11.568 kb on - strand, within GFF747at 11.759 kb on + strandat 11.760 kb on - strandat 11.760 kb on - strandat 11.798 kb on + strandat 11.798 kb on + strandat 11.798 kb on + strandat 11.798 kb on + strandat 11.798 kb on + strandat 11.798 kb on + strandat 11.798 kb on + strandat 11.798 kb on + strandat 11.799 kb on - strandat 11.799 kb on - strandat 11.799 kb on - strandat 11.799 kb on - strandat 11.799 kb on - strandat 11.799 kb on - strandat 11.799 kb on - strandat 11.799 kb on - strandat 12.515 kb on - strandat 12.515 kb on - strandat 12.515 kb on - strandat 12.562 kb on + strand, within GFF749at 12.563 kb on - strand, within GFF749at 12.563 kb on - strand, within GFF749at 12.580 kb on + strand, within GFF749at 12.694 kb on + strand, within GFF749at 12.695 kb on - strand, within GFF749at 12.695 kb on - strand, within GFF749at 12.695 kb on - strand, within GFF749

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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9,454 - +0.4
9,630 + GFF746 0.22 +0.1
9,834 + GFF746 0.43 +0.4
9,835 - GFF746 0.43 -0.8
9,835 - GFF746 0.43 -0.9
10,060 - GFF746 0.66 -1.1
10,122 + GFF746 0.72 -0.2
10,122 + GFF746 0.72 -0.8
10,123 - GFF746 0.72 -0.7
10,123 - GFF746 0.72 -0.2
10,123 - GFF746 0.72 -1.1
10,123 - GFF746 0.72 -1.3
10,386 + -1.0
10,497 - -0.3
10,562 + GFF747 0.10 -0.7
10,562 + GFF747 0.10 -0.6
10,562 + GFF747 0.10 -0.8
10,562 + GFF747 0.10 +1.5
10,562 + GFF747 0.10 -0.7
10,563 - GFF747 0.10 -0.2
10,563 - GFF747 0.10 -1.6
10,563 - GFF747 0.10 -0.4
10,585 + GFF747 0.12 -1.4
10,586 - GFF747 0.12 -0.5
10,629 - GFF747 0.15 -1.3
10,629 - GFF747 0.15 +0.6
10,778 + GFF747 0.27 -0.5
10,812 - GFF747 0.29 +1.2
10,910 + GFF747 0.37 +0.1
10,910 + GFF747 0.37 -1.4
10,911 - GFF747 0.37 +1.1
10,911 - GFF747 0.37 +0.1
10,911 - GFF747 0.37 -0.4
10,911 - GFF747 0.37 -2.1
10,946 + GFF747 0.40 +0.1
10,946 + GFF747 0.40 +0.8
10,946 + GFF747 0.40 -0.4
10,946 + GFF747 0.40 -0.9
10,946 + GFF747 0.40 -1.8
10,946 + GFF747 0.40 -0.6
10,949 + GFF747 0.40 +0.2
10,949 + GFF747 0.40 -0.6
10,949 + GFF747 0.40 -0.1
10,949 + GFF747 0.40 -0.1
10,949 + GFF747 0.40 -0.4
10,949 + GFF747 0.40 -0.5
10,949 + GFF747 0.40 -1.3
10,949 + GFF747 0.40 -1.0
10,949 + GFF747 0.40 +1.4
10,950 - GFF747 0.40 +0.7
10,950 - GFF747 0.40 -1.2
10,950 - GFF747 0.40 -0.2
10,950 - GFF747 0.40 +0.9
10,950 - GFF747 0.40 -0.9
10,950 - GFF747 0.40 -0.2
10,950 - GFF747 0.40 -0.4
10,950 - GFF747 0.40 -0.6
10,950 - GFF747 0.40 +0.4
11,204 + GFF747 0.59 -0.1
11,244 - GFF747 0.62 -0.5
11,531 + GFF747 0.84 -1.0
11,531 + GFF747 0.84 -0.6
11,532 - GFF747 0.85 +0.1
11,532 - GFF747 0.85 +1.4
11,567 + GFF747 0.87 -0.2
11,567 + GFF747 0.87 +0.1
11,568 - GFF747 0.87 -0.0
11,568 - GFF747 0.87 -1.2
11,759 + -1.3
11,760 - +0.8
11,760 - -0.5
11,798 + -0.6
11,798 + -1.0
11,798 + -1.2
11,798 + +1.1
11,798 + -0.4
11,798 + -1.8
11,798 + +0.7
11,798 + -0.2
11,799 - -0.2
11,799 - -2.5
11,799 - -2.4
11,799 - -1.1
11,799 - -0.2
11,799 - -0.8
11,799 - -0.9
11,799 - -0.4
12,515 - -0.1
12,515 - -1.9
12,515 - -0.6
12,562 + GFF749 0.11 +0.3
12,563 - GFF749 0.11 +0.6
12,563 - GFF749 0.11 -1.0
12,580 + GFF749 0.13 -0.9
12,694 + GFF749 0.24 -0.8
12,695 - GFF749 0.24 -1.6
12,695 - GFF749 0.24 -1.2
12,695 - GFF749 0.24 -0.3

Or see this region's nucleotide sequence