Strain Fitness in Variovorax sp. SCN45 around GFF7264

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7263 and GFF7264 are separated by 505 nucleotidesGFF7264 and GFF7265 are separated by 21 nucleotides GFF7263 - Malate dehydrogenase (EC 1.1.1.37), at 9,725 to 10,819 GFF7263 GFF7264 - Gluconolactonase (EC 3.1.1.17), at 11,325 to 12,170 GFF7264 GFF7265 - hypothetical protein, not 6-phosphogluconolactonase, at 12,192 to 13,304 GFF7265 Position (kb) 11 12 13Strain fitness (log2 ratio) -1 0 1at 10.568 kb on + strand, within GFF7263at 10.854 kb on + strandat 10.985 kb on + strandat 10.985 kb on + strandat 11.031 kb on + strandat 11.031 kb on + strandat 11.063 kb on - strandat 11.074 kb on + strandat 11.265 kb on - strandat 11.340 kb on + strandat 11.340 kb on + strandat 11.340 kb on + strandat 11.453 kb on + strand, within GFF7264at 11.794 kb on - strand, within GFF7264at 11.794 kb on - strand, within GFF7264at 11.970 kb on - strand, within GFF7264at 12.126 kb on + strandat 12.127 kb on - strandat 12.303 kb on + strandat 12.513 kb on + strand, within GFF7265at 12.514 kb on - strand, within GFF7265at 12.540 kb on + strand, within GFF7265at 12.540 kb on + strand, within GFF7265at 12.688 kb on - strand, within GFF7265at 12.837 kb on + strand, within GFF7265at 12.838 kb on - strand, within GFF7265at 12.943 kb on - strand, within GFF7265

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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10,568 + GFF7263 0.77 -0.2
10,854 + +0.2
10,985 + +0.1
10,985 + -0.1
11,031 + +0.1
11,031 + -0.6
11,063 - +0.5
11,074 + -1.3
11,265 - -0.3
11,340 + -0.8
11,340 + +0.2
11,340 + -0.5
11,453 + GFF7264 0.15 -0.8
11,794 - GFF7264 0.55 -0.3
11,794 - GFF7264 0.55 -1.5
11,970 - GFF7264 0.76 -1.1
12,126 + +1.6
12,127 - -0.6
12,303 + -1.1
12,513 + GFF7265 0.29 -0.4
12,514 - GFF7265 0.29 +0.2
12,540 + GFF7265 0.31 -0.1
12,540 + GFF7265 0.31 -0.1
12,688 - GFF7265 0.45 -0.8
12,837 + GFF7265 0.58 +1.5
12,838 - GFF7265 0.58 +0.4
12,943 - GFF7265 0.67 -0.2

Or see this region's nucleotide sequence