Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF713 and GFF714 are separated by 33 nucleotides GFF714 and GFF715 are separated by 34 nucleotides GFF715 and GFF716 overlap by 4 nucleotides GFF716 and GFF717 are separated by 28 nucleotides
GFF713 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79), at 772,018 to 773,403
GFF713
GFF714 - no description, at 773,437 to 773,580
GFF714
GFF715 - hypothetical protein, at 773,615 to 774,958
GFF715
GFF716 - no description, at 774,955 to 775,608
GFF716
GFF717 - Enoyl-CoA hydratase (EC 4.2.1.17), at 775,637 to 776,428
GFF717
Position (kb) 773
774
775 Strain fitness (log2 ratio) -2
-1
0
1 at 772.844 kb on + strand, within GFF713 at 772.844 kb on + strand, within GFF713 at 772.845 kb on - strand, within GFF713 at 772.845 kb on - strand, within GFF713 at 772.845 kb on - strand, within GFF713 at 772.935 kb on + strand, within GFF713 at 772.935 kb on + strand, within GFF713 at 773.030 kb on + strand, within GFF713 at 773.183 kb on + strand, within GFF713 at 773.442 kb on + strand at 773.442 kb on + strand at 773.442 kb on + strand at 773.442 kb on + strand at 773.442 kb on + strand at 773.442 kb on + strand at 773.443 kb on - strand at 773.443 kb on - strand at 773.443 kb on - strand at 773.443 kb on - strand at 773.443 kb on - strand at 773.443 kb on - strand at 773.443 kb on - strand at 773.443 kb on - strand at 773.489 kb on + strand, within GFF714 at 773.490 kb on - strand, within GFF714 at 773.490 kb on - strand, within GFF714 at 773.510 kb on + strand, within GFF714 at 773.511 kb on - strand, within GFF714 at 773.531 kb on + strand, within GFF714 at 773.532 kb on - strand, within GFF714 at 773.578 kb on + strand at 773.578 kb on + strand at 773.579 kb on - strand at 773.726 kb on + strand at 773.883 kb on - strand, within GFF715 at 774.207 kb on + strand, within GFF715 at 774.208 kb on - strand, within GFF715 at 774.350 kb on + strand, within GFF715 at 774.350 kb on + strand, within GFF715 at 774.351 kb on - strand, within GFF715 at 774.351 kb on - strand, within GFF715 at 774.439 kb on - strand, within GFF715 at 774.614 kb on + strand, within GFF715 at 774.615 kb on - strand, within GFF715 at 774.615 kb on - strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.665 kb on + strand, within GFF715 at 774.666 kb on - strand, within GFF715 at 774.666 kb on - strand, within GFF715 at 774.666 kb on - strand, within GFF715 at 774.666 kb on - strand, within GFF715 at 774.666 kb on - strand, within GFF715 at 774.890 kb on + strand at 774.890 kb on + strand at 774.890 kb on + strand at 774.890 kb on + strand at 774.891 kb on - strand at 774.891 kb on - strand at 774.891 kb on - strand at 774.891 kb on - strand at 774.954 kb on + strand at 774.955 kb on - strand at 775.039 kb on + strand, within GFF716 at 775.081 kb on + strand, within GFF716 at 775.082 kb on - strand, within GFF716 at 775.138 kb on + strand, within GFF716 at 775.138 kb on + strand, within GFF716 at 775.139 kb on - strand, within GFF716 at 775.139 kb on - strand, within GFF716 at 775.139 kb on - strand, within GFF716 at 775.348 kb on + strand, within GFF716 at 775.349 kb on - strand, within GFF716 at 775.349 kb on - strand, within GFF716 at 775.349 kb on - strand, within GFF716 at 775.349 kb on - strand, within GFF716 at 775.430 kb on - strand, within GFF716 at 775.439 kb on - strand, within GFF716
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 772,844 + GFF713 0.60 -0.5 772,844 + GFF713 0.60 -0.0 772,845 - GFF713 0.60 -0.2 772,845 - GFF713 0.60 -0.4 772,845 - GFF713 0.60 -0.6 772,935 + GFF713 0.66 +0.2 772,935 + GFF713 0.66 -1.0 773,030 + GFF713 0.73 -0.8 773,183 + GFF713 0.84 -1.8 773,442 + +0.8 773,442 + +0.9 773,442 + +0.8 773,442 + -1.9 773,442 + +0.9 773,442 + -1.2 773,443 - +0.4 773,443 - -1.2 773,443 - +0.3 773,443 - -1.3 773,443 - -0.7 773,443 - +0.3 773,443 - +0.3 773,443 - -0.8 773,489 + GFF714 0.36 +0.1 773,490 - GFF714 0.37 +0.7 773,490 - GFF714 0.37 +1.5 773,510 + GFF714 0.51 -0.7 773,511 - GFF714 0.51 -0.2 773,531 + GFF714 0.65 -1.5 773,532 - GFF714 0.66 -0.7 773,578 + -0.3 773,578 + -0.8 773,579 - -0.4 773,726 + +0.2 773,883 - GFF715 0.20 -0.6 774,207 + GFF715 0.44 -1.1 774,208 - GFF715 0.44 -0.1 774,350 + GFF715 0.55 -0.4 774,350 + GFF715 0.55 +0.5 774,351 - GFF715 0.55 -2.0 774,351 - GFF715 0.55 +0.1 774,439 - GFF715 0.61 -0.8 774,614 + GFF715 0.74 -0.1 774,615 - GFF715 0.74 +0.5 774,615 - GFF715 0.74 -0.3 774,665 + GFF715 0.78 +0.0 774,665 + GFF715 0.78 -0.9 774,665 + GFF715 0.78 -0.9 774,665 + GFF715 0.78 +0.2 774,665 + GFF715 0.78 -0.1 774,665 + GFF715 0.78 -0.8 774,665 + GFF715 0.78 -1.8 774,665 + GFF715 0.78 -0.2 774,666 - GFF715 0.78 +0.3 774,666 - GFF715 0.78 -2.6 774,666 - GFF715 0.78 -0.0 774,666 - GFF715 0.78 +0.1 774,666 - GFF715 0.78 -0.7 774,890 + +1.2 774,890 + +0.1 774,890 + -0.5 774,890 + -0.2 774,891 - -0.4 774,891 - -0.1 774,891 - -0.7 774,891 - -0.1 774,954 + -0.6 774,955 - +0.2 775,039 + GFF716 0.13 +0.0 775,081 + GFF716 0.19 -0.4 775,082 - GFF716 0.19 +1.1 775,138 + GFF716 0.28 -0.9 775,138 + GFF716 0.28 -1.5 775,139 - GFF716 0.28 -0.1 775,139 - GFF716 0.28 -0.1 775,139 - GFF716 0.28 -1.1 775,348 + GFF716 0.60 +0.6 775,349 - GFF716 0.60 +0.1 775,349 - GFF716 0.60 -0.4 775,349 - GFF716 0.60 -0.7 775,349 - GFF716 0.60 +0.0 775,430 - GFF716 0.73 +0.9 775,439 - GFF716 0.74 -0.6
Or see this region's nucleotide sequence