Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7115 and GFF7116 are separated by 16 nucleotides GFF7116 and GFF7117 overlap by 4 nucleotides GFF7117 and GFF7118 overlap by 4 nucleotides GFF7118 and GFF7119 are separated by 6 nucleotides
GFF7115 - hypothetical protein, at 33,663 to 34,481
GFF7115
GFF7116 - 2,3-dihydroxybiphenyl 1,2-dioxygenase (EC 1.13.11.39), at 34,498 to 35,412
GFF7116
GFF7117 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 35,409 to 36,239
GFF7117
GFF7118 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 36,236 to 37,177
GFF7118
GFF7119 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 37,184 to 37,660
GFF7119
Position (kb)
35
36
37 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.539 kb on - strand at 34.550 kb on + strand at 34.551 kb on - strand at 34.551 kb on - strand at 34.553 kb on + strand at 34.553 kb on + strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.625 kb on + strand, within GFF7116 at 34.625 kb on + strand, within GFF7116 at 34.625 kb on + strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.790 kb on - strand, within GFF7116 at 34.790 kb on - strand, within GFF7116 at 34.863 kb on - strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 35.014 kb on + strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.046 kb on + strand, within GFF7116 at 35.047 kb on - strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.195 kb on + strand, within GFF7116 at 35.195 kb on + strand, within GFF7116 at 35.196 kb on - strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.315 kb on + strand, within GFF7116 at 35.315 kb on + strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.357 kb on + strand at 35.357 kb on + strand at 35.358 kb on - strand at 35.582 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.797 kb on + strand, within GFF7117 at 35.971 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 36.004 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.109 kb on + strand, within GFF7117 at 36.175 kb on + strand at 36.175 kb on + strand at 36.175 kb on + strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.187 kb on - strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.921 kb on - strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 37.032 kb on + strand, within GFF7118 at 37.185 kb on + strand at 37.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 34,535 + -0.0 34,535 + -0.6 34,535 + -0.6 34,535 + -0.7 34,535 + -1.0 34,535 + +0.8 34,536 - -0.4 34,536 - +0.3 34,536 - +1.2 34,536 - -0.3 34,538 + -0.5 34,538 + +0.0 34,538 + -2.0 34,538 + -1.2 34,539 - -0.3 34,550 + -0.6 34,551 - -1.5 34,551 - +0.6 34,553 + +1.4 34,553 + -0.6 34,554 - -1.7 34,554 - +0.2 34,554 - +0.2 34,554 - +0.2 34,625 + GFF7116 0.14 -0.7 34,625 + GFF7116 0.14 +0.5 34,625 + GFF7116 0.14 -0.2 34,626 - GFF7116 0.14 -0.4 34,626 - GFF7116 0.14 -0.5 34,626 - GFF7116 0.14 -0.5 34,678 + GFF7116 0.20 +0.2 34,678 + GFF7116 0.20 +0.2 34,678 + GFF7116 0.20 -0.3 34,679 - GFF7116 0.20 +0.4 34,679 - GFF7116 0.20 -0.2 34,679 - GFF7116 0.20 -0.2 34,679 - GFF7116 0.20 -0.6 34,679 - GFF7116 0.20 +0.2 34,679 - GFF7116 0.20 +0.2 34,733 + GFF7116 0.26 -0.7 34,733 + GFF7116 0.26 +0.9 34,733 + GFF7116 0.26 -2.5 34,734 - GFF7116 0.26 -0.8 34,734 - GFF7116 0.26 -0.8 34,734 - GFF7116 0.26 -0.4 34,734 - GFF7116 0.26 -0.0 34,789 + GFF7116 0.32 +0.2 34,789 + GFF7116 0.32 +0.5 34,789 + GFF7116 0.32 -0.9 34,790 - GFF7116 0.32 +1.4 34,790 - GFF7116 0.32 -0.4 34,863 - GFF7116 0.40 -0.4 34,895 + GFF7116 0.43 +0.1 34,895 + GFF7116 0.43 +0.6 34,895 + GFF7116 0.43 +0.3 34,895 + GFF7116 0.43 -1.0 34,896 - GFF7116 0.43 -0.6 34,896 - GFF7116 0.43 +0.3 34,896 - GFF7116 0.43 +0.3 35,014 + GFF7116 0.56 -2.1 35,015 - GFF7116 0.57 +0.4 35,015 - GFF7116 0.57 -0.6 35,015 - GFF7116 0.57 +0.1 35,028 - GFF7116 0.58 -0.8 35,028 - GFF7116 0.58 -1.9 35,028 - GFF7116 0.58 +0.3 35,028 - GFF7116 0.58 +0.6 35,028 - GFF7116 0.58 +1.2 35,028 - GFF7116 0.58 +0.2 35,046 + GFF7116 0.60 -1.1 35,047 - GFF7116 0.60 -0.1 35,154 + GFF7116 0.72 -1.0 35,154 + GFF7116 0.72 -0.5 35,154 + GFF7116 0.72 -0.4 35,154 + GFF7116 0.72 -0.1 35,155 - GFF7116 0.72 +1.5 35,155 - GFF7116 0.72 -0.8 35,155 - GFF7116 0.72 -1.0 35,155 - GFF7116 0.72 -0.8 35,155 - GFF7116 0.72 +0.5 35,155 - GFF7116 0.72 +0.3 35,195 + GFF7116 0.76 -1.1 35,195 + GFF7116 0.76 -1.9 35,196 - GFF7116 0.76 +0.3 35,200 + GFF7116 0.77 -0.4 35,200 + GFF7116 0.77 +0.1 35,200 + GFF7116 0.77 -0.7 35,200 + GFF7116 0.77 -0.0 35,200 + GFF7116 0.77 -0.2 35,200 + GFF7116 0.77 -0.5 35,200 + GFF7116 0.77 -0.9 35,200 + GFF7116 0.77 -0.7 35,200 + GFF7116 0.77 -0.4 35,200 + GFF7116 0.77 -0.2 35,200 + GFF7116 0.77 -0.1 35,200 + GFF7116 0.77 +0.5 35,200 + GFF7116 0.77 +0.3 35,200 + GFF7116 0.77 -0.5 35,201 - GFF7116 0.77 +0.4 35,201 - GFF7116 0.77 +0.4 35,201 - GFF7116 0.77 +1.2 35,201 - GFF7116 0.77 -2.0 35,201 - GFF7116 0.77 +0.0 35,201 - GFF7116 0.77 -0.3 35,201 - GFF7116 0.77 -0.3 35,201 - GFF7116 0.77 +0.2 35,201 - GFF7116 0.77 -0.5 35,201 - GFF7116 0.77 +0.1 35,201 - GFF7116 0.77 -0.2 35,201 - GFF7116 0.77 +0.5 35,201 - GFF7116 0.77 +0.0 35,201 - GFF7116 0.77 +0.8 35,201 - GFF7116 0.77 -0.6 35,201 - GFF7116 0.77 -0.2 35,201 - GFF7116 0.77 +0.1 35,201 - GFF7116 0.77 -0.4 35,201 - GFF7116 0.77 +0.4 35,201 - GFF7116 0.77 -0.7 35,315 + GFF7116 0.89 -0.1 35,315 + GFF7116 0.89 +0.4 35,316 - GFF7116 0.89 -0.3 35,316 - GFF7116 0.89 -0.2 35,316 - GFF7116 0.89 +1.8 35,316 - GFF7116 0.89 -0.7 35,357 + -1.0 35,357 + -0.3 35,358 - -2.2 35,582 + GFF7117 0.21 -0.4 35,583 + GFF7117 0.21 +0.8 35,583 + GFF7117 0.21 -2.6 35,583 + GFF7117 0.21 -0.1 35,583 + GFF7117 0.21 -0.5 35,583 + GFF7117 0.21 -0.1 35,583 + GFF7117 0.21 +0.2 35,584 - GFF7117 0.21 -2.6 35,584 - GFF7117 0.21 -1.7 35,584 - GFF7117 0.21 +0.0 35,584 - GFF7117 0.21 -0.5 35,584 - GFF7117 0.21 -1.1 35,584 - GFF7117 0.21 +0.0 35,584 - GFF7117 0.21 +0.8 35,584 - GFF7117 0.21 -0.8 35,584 - GFF7117 0.21 -1.5 35,584 - GFF7117 0.21 -0.1 35,584 - GFF7117 0.21 +3.1 35,771 + GFF7117 0.44 +1.6 35,771 + GFF7117 0.44 +0.3 35,771 + GFF7117 0.44 -1.1 35,771 + GFF7117 0.44 -0.5 35,771 + GFF7117 0.44 +0.6 35,771 + GFF7117 0.44 -0.5 35,771 + GFF7117 0.44 +2.4 35,772 - GFF7117 0.44 -0.3 35,772 - GFF7117 0.44 +0.6 35,772 - GFF7117 0.44 +0.6 35,772 - GFF7117 0.44 +1.2 35,797 + GFF7117 0.47 -1.5 35,971 + GFF7117 0.68 +0.3 35,992 + GFF7117 0.70 -0.8 35,992 + GFF7117 0.70 -0.3 35,992 + GFF7117 0.70 -0.1 35,992 + GFF7117 0.70 -1.0 35,993 - GFF7117 0.70 -0.5 35,993 - GFF7117 0.70 -0.1 36,004 + GFF7117 0.72 +0.6 36,094 + GFF7117 0.82 +0.2 36,094 + GFF7117 0.82 -0.4 36,095 - GFF7117 0.83 +0.9 36,095 - GFF7117 0.83 -0.1 36,095 - GFF7117 0.83 +0.2 36,095 - GFF7117 0.83 +1.0 36,109 + GFF7117 0.84 +1.4 36,175 + -1.5 36,175 + -0.9 36,175 + -0.7 36,176 - -0.1 36,176 - -0.3 36,176 - -2.0 36,176 - -0.6 36,186 + -1.2 36,186 + -0.6 36,186 + +0.0 36,186 + -0.4 36,187 - -0.8 36,230 + -0.7 36,230 + +0.1 36,230 + -0.4 36,230 + -0.2 36,230 + -2.4 36,230 + -0.4 36,231 - +0.5 36,231 - -0.2 36,231 - -1.1 36,447 + GFF7118 0.22 +0.6 36,447 + GFF7118 0.22 +1.0 36,447 + GFF7118 0.22 +1.8 36,448 - GFF7118 0.23 -0.8 36,448 - GFF7118 0.23 -0.7 36,448 - GFF7118 0.23 -1.4 36,921 - GFF7118 0.73 -0.4 36,924 + GFF7118 0.73 -0.3 36,924 + GFF7118 0.73 +0.6 36,924 + GFF7118 0.73 -0.4 36,925 - GFF7118 0.73 -0.2 36,925 - GFF7118 0.73 +0.5 36,925 - GFF7118 0.73 +0.3 36,925 - GFF7118 0.73 -1.0 36,925 - GFF7118 0.73 -0.6 36,925 - GFF7118 0.73 -0.5 36,925 - GFF7118 0.73 +2.0 36,925 - GFF7118 0.73 -0.6 36,925 - GFF7118 0.73 -0.0 36,972 + GFF7118 0.78 +0.2 36,972 + GFF7118 0.78 -0.2 36,972 + GFF7118 0.78 -0.9 36,972 + GFF7118 0.78 +0.5 36,973 - GFF7118 0.78 -0.7 36,973 - GFF7118 0.78 -0.1 37,032 + GFF7118 0.85 -0.5 37,185 + +0.2 37,185 + +0.6
Or see this region's nucleotide sequence