Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF70 and GFF71 are separated by 6 nucleotides GFF71 and GFF72 are separated by 24 nucleotides
GFF70 - Mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 86,892 to 88,403
GFF70
GFF71 - Aminoglycoside 3''-nucleotidyltransferase (EC 2.7.7.-) => ANT(3'')-Ia (AadA family), at 88,410 to 89,219
GFF71
GFF72 - D-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.-), at 89,244 to 90,377
GFF72
Position (kb) 88
89
90 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 87.459 kb on + strand, within GFF70 at 87.991 kb on - strand, within GFF70 at 88.167 kb on + strand, within GFF70 at 88.168 kb on - strand, within GFF70 at 88.168 kb on - strand, within GFF70 at 88.287 kb on + strand at 88.287 kb on + strand at 88.288 kb on - strand at 88.288 kb on - strand at 88.288 kb on - strand at 88.446 kb on + strand at 88.526 kb on - strand, within GFF71 at 88.526 kb on - strand, within GFF71 at 88.795 kb on + strand, within GFF71 at 88.795 kb on + strand, within GFF71 at 88.796 kb on - strand, within GFF71 at 89.232 kb on + strand at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 90.134 kb on - strand, within GFF72
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 87,459 + GFF70 0.38 -0.2 87,991 - GFF70 0.73 +0.6 88,167 + GFF70 0.84 +0.5 88,168 - GFF70 0.84 -0.6 88,168 - GFF70 0.84 -0.0 88,287 + -0.1 88,287 + -0.4 88,288 - -1.6 88,288 - -0.6 88,288 - -0.8 88,446 + -1.2 88,526 - GFF71 0.14 -1.6 88,526 - GFF71 0.14 -0.2 88,795 + GFF71 0.48 -1.2 88,795 + GFF71 0.48 -2.9 88,796 - GFF71 0.48 -0.8 89,232 + -0.4 89,449 + GFF72 0.18 +0.3 89,449 + GFF72 0.18 -0.6 89,449 + GFF72 0.18 +0.6 89,449 + GFF72 0.18 -0.8 89,449 + GFF72 0.18 +0.2 89,449 + GFF72 0.18 -0.2 89,450 - GFF72 0.18 +1.2 89,450 - GFF72 0.18 +0.4 89,450 - GFF72 0.18 -0.6 89,671 + GFF72 0.38 +0.9 89,671 + GFF72 0.38 -0.1 89,671 + GFF72 0.38 -0.3 89,671 + GFF72 0.38 +0.3 89,671 + GFF72 0.38 -0.0 89,672 - GFF72 0.38 -1.2 89,672 - GFF72 0.38 -0.6 89,672 - GFF72 0.38 +1.5 90,134 - GFF72 0.78 +0.6
Or see this region's nucleotide sequence