Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7014 and GFF7015 are separated by 181 nucleotides GFF7015 and GFF7016 are separated by 43 nucleotides GFF7016 and GFF7017 are separated by 199 nucleotides
GFF7014 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 29,533 to 31,068
GFF7014
GFF7015 - protein of unknown function DUF1486, at 31,250 to 31,630
GFF7015
GFF7016 - FAD-dependent oxidoreductase, at 31,674 to 33,164
GFF7016
GFF7017 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 33,364 to 34,977
GFF7017
Position (kb) 31
32
33
34 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 30.870 kb on - strand, within GFF7014 at 30.870 kb on - strand, within GFF7014 at 31.175 kb on + strand at 31.175 kb on + strand at 31.628 kb on + strand at 31.629 kb on - strand at 31.629 kb on - strand at 31.629 kb on - strand at 31.794 kb on + strand at 31.836 kb on + strand, within GFF7016 at 31.836 kb on + strand, within GFF7016 at 32.208 kb on + strand, within GFF7016 at 32.209 kb on - strand, within GFF7016 at 32.355 kb on + strand, within GFF7016 at 32.356 kb on - strand, within GFF7016 at 33.042 kb on + strand at 33.042 kb on + strand at 33.042 kb on + strand at 33.132 kb on + strand at 33.132 kb on + strand at 33.132 kb on + strand at 33.820 kb on + strand, within GFF7017 at 33.820 kb on + strand, within GFF7017 at 33.821 kb on - strand, within GFF7017 at 33.821 kb on - strand, within GFF7017 at 33.910 kb on + strand, within GFF7017 at 33.911 kb on - strand, within GFF7017 at 34.054 kb on + strand, within GFF7017
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 30,870 - GFF7014 0.87 -1.1 30,870 - GFF7014 0.87 -0.3 31,175 + +0.2 31,175 + -0.3 31,628 + -3.0 31,629 - -0.1 31,629 - -0.4 31,629 - +2.0 31,794 + -1.8 31,836 + GFF7016 0.11 -0.3 31,836 + GFF7016 0.11 -0.9 32,208 + GFF7016 0.36 -1.5 32,209 - GFF7016 0.36 -0.1 32,355 + GFF7016 0.46 -1.8 32,356 - GFF7016 0.46 +0.5 33,042 + -0.1 33,042 + +1.1 33,042 + -0.6 33,132 + -0.2 33,132 + -0.3 33,132 + -0.1 33,820 + GFF7017 0.28 -1.2 33,820 + GFF7017 0.28 +0.2 33,821 - GFF7017 0.28 -0.2 33,821 - GFF7017 0.28 +0.9 33,910 + GFF7017 0.34 -0.5 33,911 - GFF7017 0.34 -0.6 34,054 + GFF7017 0.43 -1.3
Or see this region's nucleotide sequence