Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6912 and GFF6913 are separated by 131 nucleotides GFF6913 and GFF6914 are separated by 100 nucleotides
GFF6912 - L-sorbosone dehydrogenase, at 47,595 to 48,968
GFF6912
GFF6913 - Ser-tRNA(Ala) deacylase @ Gly-tRNA(Ala) deacylase, at 49,100 to 49,873
GFF6913
GFF6914 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 49,974 to 52,148
GFF6914
Position (kb)
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 48.188 kb on + strand, within GFF6912 at 48.188 kb on + strand, within GFF6912 at 48.188 kb on + strand, within GFF6912 at 48.189 kb on - strand, within GFF6912 at 48.189 kb on - strand, within GFF6912 at 48.256 kb on + strand, within GFF6912 at 48.256 kb on + strand, within GFF6912 at 48.256 kb on + strand, within GFF6912 at 48.257 kb on - strand, within GFF6912 at 48.257 kb on - strand, within GFF6912 at 48.257 kb on - strand, within GFF6912 at 48.257 kb on - strand, within GFF6912 at 48.257 kb on - strand, within GFF6912 at 48.257 kb on - strand, within GFF6912 at 48.257 kb on - strand, within GFF6912 at 48.373 kb on + strand, within GFF6912 at 48.373 kb on + strand, within GFF6912 at 48.373 kb on + strand, within GFF6912 at 48.373 kb on + strand, within GFF6912 at 48.374 kb on - strand, within GFF6912 at 48.374 kb on - strand, within GFF6912 at 48.374 kb on - strand, within GFF6912 at 48.374 kb on - strand, within GFF6912 at 48.374 kb on - strand, within GFF6912 at 48.439 kb on + strand, within GFF6912 at 48.682 kb on + strand, within GFF6912 at 48.682 kb on + strand, within GFF6912 at 48.682 kb on + strand, within GFF6912 at 48.682 kb on + strand, within GFF6912 at 48.682 kb on + strand, within GFF6912 at 48.682 kb on + strand, within GFF6912 at 48.683 kb on - strand, within GFF6912 at 48.683 kb on - strand, within GFF6912 at 48.683 kb on - strand, within GFF6912 at 48.683 kb on - strand, within GFF6912 at 48.683 kb on - strand, within GFF6912 at 48.683 kb on - strand, within GFF6912 at 48.683 kb on - strand, within GFF6912 at 48.927 kb on + strand at 48.927 kb on + strand at 48.927 kb on + strand at 48.948 kb on + strand at 48.948 kb on + strand at 48.948 kb on + strand at 48.948 kb on + strand at 48.948 kb on + strand at 48.949 kb on - strand at 48.949 kb on - strand at 48.949 kb on - strand at 49.053 kb on + strand at 49.054 kb on - strand at 49.054 kb on - strand at 49.054 kb on - strand at 49.054 kb on - strand at 49.054 kb on - strand at 49.088 kb on + strand at 49.089 kb on - strand at 49.130 kb on + strand at 49.130 kb on + strand at 49.130 kb on + strand at 49.130 kb on + strand at 49.130 kb on + strand at 49.130 kb on + strand at 49.130 kb on + strand at 49.131 kb on - strand at 49.131 kb on - strand at 49.131 kb on - strand at 49.202 kb on + strand, within GFF6913 at 49.202 kb on + strand, within GFF6913 at 49.203 kb on - strand, within GFF6913 at 49.203 kb on - strand, within GFF6913 at 49.203 kb on - strand, within GFF6913 at 49.502 kb on + strand, within GFF6913 at 49.502 kb on + strand, within GFF6913 at 49.503 kb on - strand, within GFF6913 at 49.503 kb on - strand, within GFF6913 at 49.503 kb on - strand, within GFF6913
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 48,188 + GFF6912 0.43 -0.2 48,188 + GFF6912 0.43 +0.0 48,188 + GFF6912 0.43 +2.0 48,189 - GFF6912 0.43 -0.9 48,189 - GFF6912 0.43 -0.1 48,256 + GFF6912 0.48 -1.1 48,256 + GFF6912 0.48 +1.6 48,256 + GFF6912 0.48 -0.7 48,257 - GFF6912 0.48 +0.1 48,257 - GFF6912 0.48 +1.7 48,257 - GFF6912 0.48 -0.4 48,257 - GFF6912 0.48 -1.2 48,257 - GFF6912 0.48 +0.0 48,257 - GFF6912 0.48 -1.1 48,257 - GFF6912 0.48 +0.7 48,373 + GFF6912 0.57 -1.2 48,373 + GFF6912 0.57 +0.4 48,373 + GFF6912 0.57 +0.0 48,373 + GFF6912 0.57 -0.3 48,374 - GFF6912 0.57 -2.1 48,374 - GFF6912 0.57 -0.1 48,374 - GFF6912 0.57 +1.7 48,374 - GFF6912 0.57 +0.2 48,374 - GFF6912 0.57 -0.5 48,439 + GFF6912 0.61 -0.4 48,682 + GFF6912 0.79 -0.2 48,682 + GFF6912 0.79 +0.1 48,682 + GFF6912 0.79 -0.6 48,682 + GFF6912 0.79 +0.7 48,682 + GFF6912 0.79 -0.0 48,682 + GFF6912 0.79 -0.2 48,683 - GFF6912 0.79 -1.1 48,683 - GFF6912 0.79 -2.5 48,683 - GFF6912 0.79 -2.3 48,683 - GFF6912 0.79 +0.2 48,683 - GFF6912 0.79 -0.4 48,683 - GFF6912 0.79 -0.5 48,683 - GFF6912 0.79 +0.7 48,927 + -1.2 48,927 + +0.0 48,927 + -0.9 48,948 + +0.3 48,948 + -1.0 48,948 + -0.7 48,948 + -0.4 48,948 + -1.0 48,949 - -0.4 48,949 - +1.0 48,949 - -0.7 49,053 + +0.6 49,054 - -0.2 49,054 - -0.3 49,054 - -0.2 49,054 - -0.2 49,054 - +0.2 49,088 + -1.5 49,089 - +0.1 49,130 + -1.2 49,130 + +1.0 49,130 + +0.2 49,130 + +0.0 49,130 + -0.7 49,130 + -0.0 49,130 + +0.7 49,131 - +0.7 49,131 - -0.3 49,131 - -0.9 49,202 + GFF6913 0.13 +0.7 49,202 + GFF6913 0.13 +0.5 49,203 - GFF6913 0.13 +0.6 49,203 - GFF6913 0.13 -0.3 49,203 - GFF6913 0.13 -0.5 49,502 + GFF6913 0.52 +0.3 49,502 + GFF6913 0.52 -0.5 49,503 - GFF6913 0.52 +0.0 49,503 - GFF6913 0.52 +0.2 49,503 - GFF6913 0.52 -0.3
Or see this region's nucleotide sequence