Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6817 and GFF6818 are separated by 732 nucleotides
GFF6817 - hypothetical protein, at 3 to 1,352
GFF6817
GFF6818 - hypothetical protein, at 2,085 to 5,882
GFF6818
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 0.073 kb on + strand at 0.073 kb on + strand at 0.094 kb on + strand at 0.094 kb on + strand at 0.095 kb on - strand at 0.095 kb on - strand at 0.095 kb on - strand at 0.134 kb on + strand at 0.135 kb on - strand at 0.184 kb on - strand, within GFF6817 at 0.184 kb on - strand, within GFF6817 at 0.246 kb on + strand, within GFF6817 at 0.246 kb on + strand, within GFF6817 at 0.246 kb on + strand, within GFF6817 at 0.247 kb on - strand, within GFF6817 at 0.317 kb on + strand, within GFF6817 at 0.317 kb on - strand, within GFF6817 at 0.320 kb on + strand, within GFF6817 at 0.320 kb on + strand, within GFF6817 at 0.320 kb on + strand, within GFF6817 at 0.320 kb on + strand, within GFF6817 at 0.321 kb on - strand, within GFF6817 at 0.321 kb on - strand, within GFF6817 at 0.321 kb on - strand, within GFF6817 at 0.527 kb on + strand, within GFF6817 at 0.528 kb on - strand, within GFF6817 at 0.528 kb on - strand, within GFF6817 at 0.528 kb on - strand, within GFF6817 at 0.559 kb on + strand, within GFF6817 at 0.559 kb on + strand, within GFF6817 at 0.595 kb on + strand, within GFF6817 at 0.596 kb on - strand, within GFF6817 at 0.596 kb on - strand, within GFF6817 at 0.596 kb on - strand, within GFF6817 at 0.604 kb on + strand, within GFF6817 at 0.647 kb on + strand, within GFF6817 at 0.647 kb on + strand, within GFF6817 at 0.648 kb on - strand, within GFF6817 at 0.741 kb on - strand, within GFF6817 at 0.741 kb on - strand, within GFF6817 at 0.744 kb on + strand, within GFF6817 at 0.744 kb on + strand, within GFF6817 at 0.744 kb on + strand, within GFF6817 at 0.744 kb on + strand, within GFF6817 at 0.744 kb on + strand, within GFF6817 at 0.744 kb on + strand, within GFF6817 at 0.745 kb on - strand, within GFF6817 at 0.745 kb on - strand, within GFF6817 at 0.745 kb on - strand, within GFF6817 at 0.745 kb on - strand, within GFF6817 at 0.804 kb on - strand, within GFF6817 at 0.816 kb on + strand, within GFF6817 at 0.816 kb on + strand, within GFF6817 at 0.816 kb on + strand, within GFF6817 at 0.816 kb on + strand, within GFF6817 at 0.817 kb on - strand, within GFF6817 at 0.817 kb on - strand, within GFF6817 at 0.817 kb on - strand, within GFF6817 at 0.817 kb on - strand, within GFF6817 at 0.914 kb on - strand, within GFF6817 at 0.920 kb on + strand, within GFF6817 at 0.920 kb on + strand, within GFF6817 at 0.920 kb on + strand, within GFF6817 at 0.920 kb on + strand, within GFF6817 at 0.921 kb on - strand, within GFF6817 at 0.921 kb on - strand, within GFF6817 at 0.921 kb on - strand, within GFF6817 at 0.921 kb on - strand, within GFF6817 at 0.921 kb on - strand, within GFF6817 at 0.930 kb on + strand, within GFF6817 at 0.931 kb on - strand, within GFF6817 at 0.996 kb on - strand, within GFF6817 at 0.996 kb on - strand, within GFF6817 at 1.025 kb on + strand, within GFF6817 at 1.026 kb on - strand, within GFF6817 at 1.026 kb on - strand, within GFF6817 at 1.032 kb on - strand, within GFF6817 at 1.035 kb on + strand, within GFF6817 at 1.035 kb on + strand, within GFF6817 at 1.036 kb on - strand, within GFF6817 at 1.036 kb on - strand, within GFF6817 at 1.043 kb on + strand, within GFF6817 at 1.044 kb on - strand, within GFF6817 at 1.044 kb on - strand, within GFF6817 at 1.044 kb on - strand, within GFF6817 at 1.044 kb on - strand, within GFF6817 at 1.044 kb on - strand, within GFF6817 at 1.044 kb on - strand, within GFF6817 at 1.047 kb on + strand, within GFF6817 at 1.048 kb on - strand, within GFF6817 at 1.048 kb on - strand, within GFF6817 at 1.048 kb on - strand, within GFF6817 at 1.100 kb on + strand, within GFF6817 at 1.100 kb on + strand, within GFF6817 at 1.101 kb on - strand, within GFF6817 at 1.101 kb on - strand, within GFF6817 at 1.150 kb on - strand, within GFF6817 at 1.150 kb on - strand, within GFF6817 at 1.193 kb on + strand, within GFF6817 at 1.193 kb on + strand, within GFF6817 at 1.194 kb on - strand, within GFF6817 at 1.194 kb on - strand, within GFF6817 at 1.197 kb on + strand, within GFF6817 at 1.198 kb on - strand, within GFF6817 at 1.198 kb on - strand, within GFF6817 at 1.198 kb on - strand, within GFF6817 at 1.274 kb on - strand at 1.365 kb on + strand at 1.365 kb on + strand at 1.366 kb on - strand at 1.440 kb on + strand at 1.441 kb on - strand at 1.448 kb on + strand at 1.448 kb on + strand at 1.448 kb on + strand at 1.492 kb on + strand at 1.493 kb on - strand at 1.493 kb on - strand at 1.493 kb on - strand at 1.493 kb on - strand at 1.493 kb on - strand at 1.567 kb on + strand at 1.567 kb on + strand at 1.677 kb on + strand at 1.790 kb on - strand at 1.826 kb on + strand at 1.826 kb on + strand at 1.826 kb on + strand at 1.826 kb on + strand at 1.826 kb on + strand at 1.826 kb on + strand at 1.827 kb on - strand at 1.827 kb on - strand at 1.828 kb on + strand at 1.829 kb on - strand at 1.982 kb on + strand at 1.983 kb on - strand at 1.983 kb on - strand at 1.983 kb on - strand at 1.993 kb on + strand at 2.228 kb on - strand at 2.245 kb on - strand at 2.245 kb on - strand at 2.245 kb on - strand at 2.345 kb on + strand at 2.345 kb on + strand at 2.345 kb on + strand at 2.346 kb on - strand at 2.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 73 + +0.1 73 + -1.8 94 + -0.6 94 + +0.8 95 - +0.1 95 - -1.0 95 - -0.6 134 + -2.2 135 - -2.1 184 - GFF6817 0.13 +0.3 184 - GFF6817 0.13 -0.6 246 + GFF6817 0.18 -1.0 246 + GFF6817 0.18 +0.8 246 + GFF6817 0.18 -0.7 247 - GFF6817 0.18 -0.6 317 + GFF6817 0.23 +0.2 317 - GFF6817 0.23 -0.7 320 + GFF6817 0.23 -0.2 320 + GFF6817 0.23 -0.6 320 + GFF6817 0.23 +0.1 320 + GFF6817 0.23 +0.9 321 - GFF6817 0.24 -0.7 321 - GFF6817 0.24 -0.2 321 - GFF6817 0.24 -0.2 527 + GFF6817 0.39 +0.8 528 - GFF6817 0.39 +0.5 528 - GFF6817 0.39 +0.5 528 - GFF6817 0.39 +0.3 559 + GFF6817 0.41 +0.2 559 + GFF6817 0.41 -1.7 595 + GFF6817 0.44 -0.7 596 - GFF6817 0.44 +0.8 596 - GFF6817 0.44 +0.6 596 - GFF6817 0.44 +0.5 604 + GFF6817 0.45 -0.3 647 + GFF6817 0.48 +0.2 647 + GFF6817 0.48 -0.0 648 - GFF6817 0.48 -0.9 741 - GFF6817 0.55 -0.9 741 - GFF6817 0.55 -0.3 744 + GFF6817 0.55 -0.1 744 + GFF6817 0.55 -1.2 744 + GFF6817 0.55 +0.2 744 + GFF6817 0.55 +0.4 744 + GFF6817 0.55 +0.1 744 + GFF6817 0.55 +0.0 745 - GFF6817 0.55 +0.3 745 - GFF6817 0.55 -0.3 745 - GFF6817 0.55 -0.7 745 - GFF6817 0.55 +0.7 804 - GFF6817 0.59 -0.3 816 + GFF6817 0.60 -0.3 816 + GFF6817 0.60 +0.1 816 + GFF6817 0.60 +1.5 816 + GFF6817 0.60 +0.0 817 - GFF6817 0.60 -0.3 817 - GFF6817 0.60 -0.8 817 - GFF6817 0.60 +3.6 817 - GFF6817 0.60 +0.6 914 - GFF6817 0.67 +0.2 920 + GFF6817 0.68 -0.3 920 + GFF6817 0.68 -0.7 920 + GFF6817 0.68 +0.3 920 + GFF6817 0.68 -0.4 921 - GFF6817 0.68 -0.2 921 - GFF6817 0.68 -0.6 921 - GFF6817 0.68 +0.1 921 - GFF6817 0.68 +0.3 921 - GFF6817 0.68 -0.6 930 + GFF6817 0.69 -0.4 931 - GFF6817 0.69 +0.2 996 - GFF6817 0.74 -1.1 996 - GFF6817 0.74 -0.3 1,025 + GFF6817 0.76 +0.5 1,026 - GFF6817 0.76 -0.5 1,026 - GFF6817 0.76 +0.0 1,032 - GFF6817 0.76 -1.0 1,035 + GFF6817 0.76 -1.6 1,035 + GFF6817 0.76 -0.6 1,036 - GFF6817 0.77 -0.3 1,036 - GFF6817 0.77 +0.3 1,043 + GFF6817 0.77 -2.3 1,044 - GFF6817 0.77 -0.6 1,044 - GFF6817 0.77 +0.2 1,044 - GFF6817 0.77 +0.6 1,044 - GFF6817 0.77 -0.3 1,044 - GFF6817 0.77 +0.3 1,044 - GFF6817 0.77 +1.3 1,047 + GFF6817 0.77 -0.1 1,048 - GFF6817 0.77 +0.2 1,048 - GFF6817 0.77 -0.6 1,048 - GFF6817 0.77 +1.3 1,100 + GFF6817 0.81 +0.8 1,100 + GFF6817 0.81 -0.0 1,101 - GFF6817 0.81 -0.6 1,101 - GFF6817 0.81 +0.0 1,150 - GFF6817 0.85 -0.5 1,150 - GFF6817 0.85 -0.2 1,193 + GFF6817 0.88 +1.5 1,193 + GFF6817 0.88 -1.0 1,194 - GFF6817 0.88 -0.3 1,194 - GFF6817 0.88 -0.0 1,197 + GFF6817 0.88 +0.5 1,198 - GFF6817 0.89 +0.0 1,198 - GFF6817 0.89 +0.4 1,198 - GFF6817 0.89 -0.3 1,274 - +0.2 1,365 + +1.0 1,365 + -0.2 1,366 - +0.3 1,440 + +0.3 1,441 - +3.1 1,448 + -0.7 1,448 + -1.1 1,448 + -1.3 1,492 + -0.6 1,493 - +0.1 1,493 - -0.6 1,493 - +0.0 1,493 - -0.1 1,493 - -1.6 1,567 + -0.7 1,567 + -1.2 1,677 + -2.1 1,790 - +1.1 1,826 + -2.3 1,826 + -1.2 1,826 + -2.3 1,826 + +1.3 1,826 + -0.4 1,826 + -0.3 1,827 - -0.7 1,827 - -1.3 1,828 + +0.4 1,829 - -0.1 1,982 + -0.3 1,983 - -1.2 1,983 - -1.7 1,983 - -0.0 1,993 + -0.7 2,228 - +1.9 2,245 - -1.5 2,245 - -0.2 2,245 - -0.8 2,345 + -0.1 2,345 + -0.8 2,345 + +0.8 2,346 - -0.3 2,346 - -0.5
Or see this region's nucleotide sequence