Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6699 and GFF6700 are separated by 191 nucleotides GFF6700 and GFF6701 overlap by 4 nucleotides
GFF6699 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,899 to 8,065
GFF6699
GFF6700 - Single-stranded-DNA-specific exonuclease RecJ, at 8,257 to 9,984
GFF6700
GFF6701 - Regulatory protein, RpfE type, at 9,981 to 10,949
GFF6701
Position (kb) 8
9
10 Strain fitness (log2 ratio) -2
-1
0
1
2 at 7.631 kb on + strand, within GFF6699 at 7.631 kb on + strand, within GFF6699 at 7.632 kb on - strand, within GFF6699 at 7.632 kb on - strand, within GFF6699 at 7.632 kb on - strand, within GFF6699 at 7.632 kb on - strand, within GFF6699 at 7.756 kb on - strand, within GFF6699 at 7.756 kb on - strand, within GFF6699 at 7.971 kb on + strand at 7.971 kb on + strand at 7.971 kb on + strand at 7.971 kb on + strand at 7.971 kb on + strand at 7.971 kb on + strand at 7.971 kb on + strand at 7.971 kb on + strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.974 kb on + strand at 7.974 kb on + strand at 7.975 kb on - strand at 7.975 kb on - strand at 8.046 kb on + strand at 8.046 kb on + strand at 8.046 kb on + strand at 8.046 kb on + strand at 8.047 kb on - strand at 8.258 kb on + strand at 8.258 kb on + strand at 8.259 kb on - strand at 8.259 kb on - strand at 8.259 kb on - strand at 8.537 kb on + strand, within GFF6700 at 8.537 kb on + strand, within GFF6700 at 8.537 kb on + strand, within GFF6700 at 8.538 kb on - strand, within GFF6700 at 9.398 kb on + strand, within GFF6700 at 9.398 kb on + strand, within GFF6700 at 9.398 kb on + strand, within GFF6700 at 9.398 kb on + strand, within GFF6700 at 9.399 kb on - strand, within GFF6700 at 9.399 kb on - strand, within GFF6700 at 9.674 kb on + strand, within GFF6700 at 9.674 kb on + strand, within GFF6700 at 9.675 kb on - strand, within GFF6700 at 9.750 kb on - strand, within GFF6700 at 9.985 kb on - strand at 10.066 kb on + strand at 10.066 kb on + strand at 10.067 kb on - strand at 10.067 kb on - strand at 10.123 kb on + strand, within GFF6701 at 10.351 kb on + strand, within GFF6701 at 10.352 kb on - strand, within GFF6701 at 10.352 kb on - strand, within GFF6701 at 10.955 kb on + strand at 10.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 7,631 + GFF6699 0.63 -0.6 7,631 + GFF6699 0.63 +0.6 7,632 - GFF6699 0.63 -1.1 7,632 - GFF6699 0.63 -0.6 7,632 - GFF6699 0.63 -0.1 7,632 - GFF6699 0.63 -1.7 7,756 - GFF6699 0.73 +0.0 7,756 - GFF6699 0.73 -1.1 7,971 + +1.0 7,971 + -0.4 7,971 + +1.3 7,971 + -0.9 7,971 + -1.4 7,971 + -1.6 7,971 + -0.6 7,971 + +0.0 7,972 - -1.4 7,972 - +0.6 7,972 - -0.1 7,972 - +0.3 7,972 - +0.3 7,972 - -0.2 7,972 - +1.6 7,972 - +0.0 7,972 - -0.5 7,972 - +0.9 7,972 - -0.7 7,974 + -0.7 7,974 + -0.7 7,975 - +0.1 7,975 - -1.7 8,046 + -0.1 8,046 + +0.6 8,046 + -0.7 8,046 + -1.1 8,047 - -0.3 8,258 + +0.7 8,258 + -0.1 8,259 - +1.0 8,259 - +0.3 8,259 - +1.0 8,537 + GFF6700 0.16 -0.4 8,537 + GFF6700 0.16 -1.2 8,537 + GFF6700 0.16 -1.4 8,538 - GFF6700 0.16 -0.5 9,398 + GFF6700 0.66 -1.6 9,398 + GFF6700 0.66 -0.7 9,398 + GFF6700 0.66 -1.6 9,398 + GFF6700 0.66 -1.0 9,399 - GFF6700 0.66 -2.4 9,399 - GFF6700 0.66 -0.2 9,674 + GFF6700 0.82 +0.3 9,674 + GFF6700 0.82 +0.1 9,675 - GFF6700 0.82 -0.4 9,750 - GFF6700 0.86 -0.9 9,985 - -1.0 10,066 + +0.0 10,066 + +0.4 10,067 - -0.0 10,067 - +0.0 10,123 + GFF6701 0.15 +0.6 10,351 + GFF6701 0.38 +1.4 10,352 - GFF6701 0.38 +0.9 10,352 - GFF6701 0.38 +0.4 10,955 + +2.6 10,956 - -2.4
Or see this region's nucleotide sequence