Strain Fitness in Variovorax sp. SCN45 around GFF6564

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
100	+				-0.8
100	+				+0.2
100	+				-0.5
101	-				+0.3
101	-				+1.2
101	-				-0.3
123	-				-0.1
150	-				-0.7
248	+		GFF6563	0.19	-0.1
248	+		GFF6563	0.19	-0.2
248	+		GFF6563	0.19	-0.4
248	+		GFF6563	0.19	-1.2
249	-		GFF6563	0.19	-0.2
249	-		GFF6563	0.19	-0.9
398	+		GFF6563	0.36	+0.1
398	+		GFF6563	0.36	+0.3
398	+		GFF6563	0.36	+0.5
399	-		GFF6563	0.36	-0.6
399	-		GFF6563	0.36	-1.4
541	+		GFF6563	0.53	-0.4
655	+		GFF6563	0.66	-0.2
656	-		GFF6563	0.66	-0.2
656	-		GFF6563	0.66	+0.2
656	-		GFF6563	0.66	-1.0
656	-		GFF6563	0.66	-0.4
656	-		GFF6563	0.66	-0.3
871	+				-0.4
872	-				+0.4
878	+				-1.2
878	+				-1.1
878	+				-0.2
879	-				-0.4
879	-				-0.5
879	-				+0.0
879	-				-0.7
879	-				+0.3
879	-				-0.1
883	-				+0.4
1,054	+				+0.2
1,054	+				+0.1
1,055	-				-0.4
1,055	-				-0.5
1,084	-				-0.7
1,120	+				-1.6
1,120	+				+0.2
1,120	+				-0.1
1,121	-				-0.6
1,121	-				-0.6
1,121	-				+0.2
1,121	-				-0.3
1,121	-				-0.9
1,123	+				-0.5
1,123	+				+0.1
1,124	-				+1.8
1,124	-				-0.7
1,124	-				-1.4
1,124	-				-0.0
1,130	+				+0.3
1,130	+				-0.1
1,131	-				-0.1
1,150	+				-0.1
1,150	+				-0.3
1,150	+				-0.3
1,151	-				+0.4
1,325	+		GFF6564	0.17	-1.8
1,326	-		GFF6564	0.17	+1.2
1,340	+		GFF6564	0.18	-0.7
1,341	-		GFF6564	0.18	-0.7
1,341	-		GFF6564	0.18	-0.2
1,341	-		GFF6564	0.18	-0.6
1,341	-		GFF6564	0.18	-0.2
1,371	+		GFF6564	0.19	-0.8
1,402	-		GFF6564	0.20	+0.4
1,419	+		GFF6564	0.21	+0.2
1,419	+		GFF6564	0.21	-0.5
1,420	-		GFF6564	0.21	-0.5
1,586	+		GFF6564	0.29	-0.1
1,587	-		GFF6564	0.29	-0.3
1,823	+		GFF6564	0.39	+0.0
1,823	+		GFF6564	0.39	-0.0
1,823	+		GFF6564	0.39	-0.1
1,824	-		GFF6564	0.39	-0.1
1,824	-		GFF6564	0.39	+0.1
1,847	+		GFF6564	0.40	+0.5
1,848	-		GFF6564	0.40	-0.4
1,881	+		GFF6564	0.42	-0.3
1,882	-		GFF6564	0.42	-0.3
1,934	-		GFF6564	0.44	-1.4
2,149	-		GFF6564	0.54	-1.2
2,149	-		GFF6564	0.54	-0.6
2,149	-		GFF6564	0.54	-0.5
2,223	+		GFF6564	0.57	+0.6
2,223	+		GFF6564	0.57	-0.8
2,223	+		GFF6564	0.57	+0.2
2,224	-		GFF6564	0.57	-1.2
2,224	-		GFF6564	0.57	+0.1
2,243	+		GFF6564	0.58	-0.4
2,261	-		GFF6564	0.58	-0.2
2,267	+		GFF6564	0.59	-1.2
2,289	+		GFF6564	0.60	-0.5
2,290	-		GFF6564	0.60	-0.1
2,290	-		GFF6564	0.60	-0.6
2,290	-		GFF6564	0.60	-0.5
2,381	+		GFF6564	0.64	-1.8
2,381	+		GFF6564	0.64	+0.6
2,382	-		GFF6564	0.64	-0.8
2,382	-		GFF6564	0.64	+0.5
2,451	+		GFF6564	0.67	-0.0
2,452	-		GFF6564	0.67	-0.5
2,452	-		GFF6564	0.67	-0.2
2,452	-		GFF6564	0.67	-0.1
2,580	+		GFF6564	0.73	-0.0
2,581	-		GFF6564	0.73	+0.3
2,581	-		GFF6564	0.73	-0.4
2,745	+		GFF6564	0.80	+0.1
2,745	+		GFF6564	0.80	-1.0
2,745	+		GFF6564	0.80	-0.3
2,745	+		GFF6564	0.80	-0.1
2,745	+		GFF6564	0.80	-0.1
2,745	+		GFF6564	0.80	+0.5
2,745	+		GFF6564	0.80	+0.4
2,745	+		GFF6564	0.80	-0.6
2,745	+		GFF6564	0.80	-0.7
2,746	-		GFF6564	0.80	-0.7
2,746	-		GFF6564	0.80	-0.5
2,746	-		GFF6564	0.80	-0.4
2,746	-		GFF6564	0.80	-0.4
2,746	-		GFF6564	0.80	+0.2
2,746	-		GFF6564	0.80	-0.9
2,746	-		GFF6564	0.80	-0.3
2,748	-		GFF6564	0.80	-1.4
2,748	-		GFF6564	0.80	-0.5
2,748	-		GFF6564	0.80	+0.6
2,845	-		GFF6564	0.84	-0.8
2,848	+		GFF6564	0.84	-0.2
2,849	-		GFF6564	0.85	-1.8
2,849	-		GFF6564	0.85	-0.9
2,849	-		GFF6564	0.85	+1.7
2,849	-		GFF6564	0.85	-0.5
2,849	-		GFF6564	0.85	+0.4
2,982	+				-0.5
2,982	+				-0.9
2,983	-				+0.7
2,983	-				+0.2
3,025	-				-0.9
3,063	+				+0.6
3,063	+				+0.1
3,064	-				-0.7
3,064	-				-0.5
3,067	+				+0.4
3,068	-				-2.0
3,102	+				+0.1
3,102	+				-0.6
3,103	-				-0.0
3,103	-				-0.1
3,103	-				-0.9
3,103	-				-0.9
3,103	-				-0.4
3,103	-				+0.4
3,349	+				+0.8
3,350	-				-0.3
3,350	-				+0.4
3,350	-				-0.5
3,350	-				-0.8
3,350	-				+0.4
3,350	-				+1.4
3,377	-				-0.5
3,426	+				-0.9
3,529	+				+0.2
3,529	+				-1.3
3,530	-				-1.7
3,530	-				+0.0
3,530	-				-1.7
3,530	-				-0.7
3,651	+		GFF6565	0.12	+0.2
3,651	+		GFF6565	0.12	-0.6
3,651	+		GFF6565	0.12	+1.4
3,651	+		GFF6565	0.12	-0.5
3,651	+		GFF6565	0.12	-0.7
3,651	+		GFF6565	0.12	-0.5
3,651	+		GFF6565	0.12	-0.3
3,652	-		GFF6565	0.12	+0.6
3,652	-		GFF6565	0.12	-0.9
3,705	+		GFF6565	0.17	-0.4
3,714	+		GFF6565	0.17	-0.9
3,714	+		GFF6565	0.17	+0.4
3,846	-		GFF6565	0.28	+0.5
4,150	-		GFF6565	0.52	+0.7
4,150	-		GFF6565	0.52	+2.4
4,166	-		GFF6565	0.54	+0.8