Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6511 and GFF6512 are separated by 2 nucleotides GFF6512 and GFF6513 are separated by 159 nucleotides GFF6513 and GFF6514 are separated by 309 nucleotides
GFF6511 - Haloacid dehalogenase, type II (EC 3.8.1.2), at 6,982 to 7,710
GFF6511
GFF6512 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 7,713 to 8,348
GFF6512
GFF6513 - Phosphogluconate dehydratase (EC 4.2.1.12), at 8,508 to 10,340
GFF6513
GFF6514 - Short-chain dehydrogenase, at 10,650 to 11,471
GFF6514
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 7.966 kb on + strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 8.370 kb on + strand at 8.371 kb on - strand at 8.769 kb on + strand, within GFF6513 at 8.770 kb on - strand, within GFF6513 at 9.122 kb on + strand, within GFF6513 at 9.123 kb on - strand, within GFF6513 at 9.317 kb on - strand, within GFF6513 at 9.317 kb on - strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.659 kb on - strand, within GFF6513 at 9.659 kb on - strand, within GFF6513 at 9.659 kb on - strand, within GFF6513 at 9.685 kb on + strand, within GFF6513 at 9.686 kb on - strand, within GFF6513 at 10.079 kb on - strand, within GFF6513 at 10.108 kb on + strand, within GFF6513 at 10.109 kb on - strand, within GFF6513 at 10.261 kb on + strand at 10.262 kb on - strand at 10.399 kb on + strand at 10.399 kb on + strand at 10.400 kb on - strand at 10.400 kb on - strand at 10.446 kb on - strand at 10.454 kb on - strand at 10.454 kb on - strand at 10.466 kb on + strand at 10.616 kb on + strand at 10.617 kb on - strand at 10.617 kb on - strand at 10.663 kb on + strand at 10.663 kb on + strand at 10.664 kb on - strand at 10.664 kb on - strand at 10.664 kb on - strand at 11.086 kb on - strand, within GFF6514 at 11.134 kb on - strand, within GFF6514 at 11.134 kb on - strand, within GFF6514 at 11.134 kb on - strand, within GFF6514 at 11.140 kb on + strand, within GFF6514 at 11.140 kb on + strand, within GFF6514 at 11.141 kb on - strand, within GFF6514 at 11.244 kb on + strand, within GFF6514 at 11.244 kb on + strand, within GFF6514 at 11.332 kb on - strand, within GFF6514 at 11.332 kb on - strand, within GFF6514 at 11.332 kb on - strand, within GFF6514
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 7,966 + GFF6512 0.40 -1.4 7,967 - GFF6512 0.40 -1.9 7,967 - GFF6512 0.40 -1.8 7,967 - GFF6512 0.40 -0.2 8,370 + +0.9 8,371 - +0.8 8,769 + GFF6513 0.14 -2.9 8,770 - GFF6513 0.14 -2.3 9,122 + GFF6513 0.33 -1.6 9,123 - GFF6513 0.34 -2.1 9,317 - GFF6513 0.44 -3.1 9,317 - GFF6513 0.44 -1.6 9,658 + GFF6513 0.63 -1.9 9,658 + GFF6513 0.63 -0.2 9,658 + GFF6513 0.63 -3.5 9,658 + GFF6513 0.63 -0.0 9,658 + GFF6513 0.63 -1.4 9,659 - GFF6513 0.63 -0.3 9,659 - GFF6513 0.63 -3.0 9,659 - GFF6513 0.63 -2.5 9,685 + GFF6513 0.64 -2.1 9,686 - GFF6513 0.64 -4.3 10,079 - GFF6513 0.86 -0.9 10,108 + GFF6513 0.87 -2.4 10,109 - GFF6513 0.87 -3.6 10,261 + -0.6 10,262 - -1.8 10,399 + -0.2 10,399 + -2.2 10,400 - +0.2 10,400 - +1.0 10,446 - +0.5 10,454 - -1.0 10,454 - +0.3 10,466 + -0.2 10,616 + +0.4 10,617 - -0.2 10,617 - +0.7 10,663 + -0.8 10,663 + -0.7 10,664 - +1.4 10,664 - +1.8 10,664 - -0.9 11,086 - GFF6514 0.53 -0.1 11,134 - GFF6514 0.59 -0.3 11,134 - GFF6514 0.59 +0.2 11,134 - GFF6514 0.59 +0.8 11,140 + GFF6514 0.60 -0.9 11,140 + GFF6514 0.60 -0.0 11,141 - GFF6514 0.60 -1.9 11,244 + GFF6514 0.72 -1.2 11,244 + GFF6514 0.72 -0.4 11,332 - GFF6514 0.83 +0.6 11,332 - GFF6514 0.83 +0.1 11,332 - GFF6514 0.83 -0.3
Or see this region's nucleotide sequence