Strain Fitness in Variovorax sp. SCN45 around GFF6513

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6511 and GFF6512 are separated by 2 nucleotidesGFF6512 and GFF6513 are separated by 159 nucleotidesGFF6513 and GFF6514 are separated by 309 nucleotides GFF6511 - Haloacid dehalogenase, type II (EC 3.8.1.2), at 6,982 to 7,710 GFF6511 GFF6512 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 7,713 to 8,348 GFF6512 GFF6513 - Phosphogluconate dehydratase (EC 4.2.1.12), at 8,508 to 10,340 GFF6513 GFF6514 - Short-chain dehydrogenase, at 10,650 to 11,471 GFF6514 Position (kb) 8 9 10 11Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 7.966 kb on + strand, within GFF6512at 7.967 kb on - strand, within GFF6512at 7.967 kb on - strand, within GFF6512at 7.967 kb on - strand, within GFF6512at 8.370 kb on + strandat 8.371 kb on - strandat 8.769 kb on + strand, within GFF6513at 8.770 kb on - strand, within GFF6513at 9.122 kb on + strand, within GFF6513at 9.123 kb on - strand, within GFF6513at 9.317 kb on - strand, within GFF6513at 9.317 kb on - strand, within GFF6513at 9.658 kb on + strand, within GFF6513at 9.658 kb on + strand, within GFF6513at 9.658 kb on + strand, within GFF6513at 9.658 kb on + strand, within GFF6513at 9.658 kb on + strand, within GFF6513at 9.659 kb on - strand, within GFF6513at 9.659 kb on - strand, within GFF6513at 9.659 kb on - strand, within GFF6513at 9.685 kb on + strand, within GFF6513at 9.686 kb on - strand, within GFF6513at 10.079 kb on - strand, within GFF6513at 10.108 kb on + strand, within GFF6513at 10.109 kb on - strand, within GFF6513at 10.261 kb on + strandat 10.262 kb on - strandat 10.399 kb on + strandat 10.399 kb on + strandat 10.400 kb on - strandat 10.400 kb on - strandat 10.446 kb on - strandat 10.454 kb on - strandat 10.454 kb on - strandat 10.466 kb on + strandat 10.616 kb on + strandat 10.617 kb on - strandat 10.617 kb on - strandat 10.663 kb on + strandat 10.663 kb on + strandat 10.664 kb on - strandat 10.664 kb on - strandat 10.664 kb on - strandat 11.086 kb on - strand, within GFF6514at 11.134 kb on - strand, within GFF6514at 11.134 kb on - strand, within GFF6514at 11.134 kb on - strand, within GFF6514at 11.140 kb on + strand, within GFF6514at 11.140 kb on + strand, within GFF6514at 11.141 kb on - strand, within GFF6514at 11.244 kb on + strand, within GFF6514at 11.244 kb on + strand, within GFF6514at 11.332 kb on - strand, within GFF6514at 11.332 kb on - strand, within GFF6514at 11.332 kb on - strand, within GFF6514

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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7,966 + GFF6512 0.40 -1.4
7,967 - GFF6512 0.40 -1.9
7,967 - GFF6512 0.40 -1.8
7,967 - GFF6512 0.40 -0.2
8,370 + +0.9
8,371 - +0.8
8,769 + GFF6513 0.14 -2.9
8,770 - GFF6513 0.14 -2.3
9,122 + GFF6513 0.33 -1.6
9,123 - GFF6513 0.34 -2.1
9,317 - GFF6513 0.44 -3.1
9,317 - GFF6513 0.44 -1.6
9,658 + GFF6513 0.63 -1.9
9,658 + GFF6513 0.63 -0.2
9,658 + GFF6513 0.63 -3.5
9,658 + GFF6513 0.63 -0.0
9,658 + GFF6513 0.63 -1.4
9,659 - GFF6513 0.63 -0.3
9,659 - GFF6513 0.63 -3.0
9,659 - GFF6513 0.63 -2.5
9,685 + GFF6513 0.64 -2.1
9,686 - GFF6513 0.64 -4.3
10,079 - GFF6513 0.86 -0.9
10,108 + GFF6513 0.87 -2.4
10,109 - GFF6513 0.87 -3.6
10,261 + -0.6
10,262 - -1.8
10,399 + -0.2
10,399 + -2.2
10,400 - +0.2
10,400 - +1.0
10,446 - +0.5
10,454 - -1.0
10,454 - +0.3
10,466 + -0.2
10,616 + +0.4
10,617 - -0.2
10,617 - +0.7
10,663 + -0.8
10,663 + -0.7
10,664 - +1.4
10,664 - +1.8
10,664 - -0.9
11,086 - GFF6514 0.53 -0.1
11,134 - GFF6514 0.59 -0.3
11,134 - GFF6514 0.59 +0.2
11,134 - GFF6514 0.59 +0.8
11,140 + GFF6514 0.60 -0.9
11,140 + GFF6514 0.60 -0.0
11,141 - GFF6514 0.60 -1.9
11,244 + GFF6514 0.72 -1.2
11,244 + GFF6514 0.72 -0.4
11,332 - GFF6514 0.83 +0.6
11,332 - GFF6514 0.83 +0.1
11,332 - GFF6514 0.83 -0.3

Or see this region's nucleotide sequence