Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6510 and GFF6511 are separated by 12 nucleotides GFF6511 and GFF6512 are separated by 2 nucleotides GFF6512 and GFF6513 are separated by 159 nucleotides
GFF6510 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 4,780 to 6,969
GFF6510
GFF6511 - Haloacid dehalogenase, type II (EC 3.8.1.2), at 6,982 to 7,710
GFF6511
GFF6512 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 7,713 to 8,348
GFF6512
GFF6513 - Phosphogluconate dehydratase (EC 4.2.1.12), at 8,508 to 10,340
GFF6513
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6.832 kb on + strand at 6.832 kb on + strand at 6.833 kb on - strand at 6.919 kb on + strand at 6.919 kb on + strand at 6.919 kb on + strand at 6.919 kb on + strand at 6.920 kb on - strand at 6.920 kb on - strand at 6.920 kb on - strand at 6.920 kb on - strand at 6.920 kb on - strand at 6.920 kb on - strand at 6.965 kb on - strand at 7.097 kb on + strand, within GFF6511 at 7.097 kb on + strand, within GFF6511 at 7.097 kb on + strand, within GFF6511 at 7.098 kb on - strand, within GFF6511 at 7.098 kb on - strand, within GFF6511 at 7.098 kb on - strand, within GFF6511 at 7.098 kb on - strand, within GFF6511 at 7.205 kb on + strand, within GFF6511 at 7.205 kb on + strand, within GFF6511 at 7.205 kb on + strand, within GFF6511 at 7.205 kb on + strand, within GFF6511 at 7.205 kb on + strand, within GFF6511 at 7.206 kb on - strand, within GFF6511 at 7.206 kb on - strand, within GFF6511 at 7.206 kb on - strand, within GFF6511 at 7.226 kb on + strand, within GFF6511 at 7.226 kb on + strand, within GFF6511 at 7.226 kb on + strand, within GFF6511 at 7.226 kb on + strand, within GFF6511 at 7.227 kb on - strand, within GFF6511 at 7.227 kb on - strand, within GFF6511 at 7.966 kb on + strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 8.370 kb on + strand at 8.371 kb on - strand at 8.769 kb on + strand, within GFF6513 at 8.770 kb on - strand, within GFF6513 at 9.122 kb on + strand, within GFF6513 at 9.123 kb on - strand, within GFF6513 at 9.317 kb on - strand, within GFF6513 at 9.317 kb on - strand, within GFF6513
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 6,832 + -0.6 6,832 + -1.0 6,833 - +1.2 6,919 + +0.4 6,919 + +1.0 6,919 + +0.3 6,919 + -0.5 6,920 - -0.3 6,920 - -2.0 6,920 - -2.2 6,920 - +2.2 6,920 - +0.0 6,920 - -0.8 6,965 - -0.1 7,097 + GFF6511 0.16 -0.1 7,097 + GFF6511 0.16 +1.0 7,097 + GFF6511 0.16 -0.7 7,098 - GFF6511 0.16 +2.4 7,098 - GFF6511 0.16 -0.2 7,098 - GFF6511 0.16 +0.1 7,098 - GFF6511 0.16 +0.1 7,205 + GFF6511 0.31 +0.6 7,205 + GFF6511 0.31 +0.4 7,205 + GFF6511 0.31 +0.0 7,205 + GFF6511 0.31 -0.2 7,205 + GFF6511 0.31 -1.2 7,206 - GFF6511 0.31 -0.4 7,206 - GFF6511 0.31 -0.8 7,206 - GFF6511 0.31 -0.5 7,226 + GFF6511 0.33 +0.3 7,226 + GFF6511 0.33 +0.6 7,226 + GFF6511 0.33 -0.6 7,226 + GFF6511 0.33 +2.0 7,227 - GFF6511 0.34 -1.1 7,227 - GFF6511 0.34 +0.5 7,966 + GFF6512 0.40 -1.4 7,967 - GFF6512 0.40 -1.9 7,967 - GFF6512 0.40 -1.8 7,967 - GFF6512 0.40 -0.2 8,370 + +0.9 8,371 - +0.8 8,769 + GFF6513 0.14 -2.9 8,770 - GFF6513 0.14 -2.3 9,122 + GFF6513 0.33 -1.6 9,123 - GFF6513 0.34 -2.1 9,317 - GFF6513 0.44 -3.1 9,317 - GFF6513 0.44 -1.6
Or see this region's nucleotide sequence